scholarly journals Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 906
Author(s):  
Alessandra Camarca ◽  
Antonio Varriale ◽  
Alessandro Capo ◽  
Angela Pennacchio ◽  
Alessia Calabrese ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Food Environment ◽  
Daily Life ◽  
Commercial Potential ◽  
Scientific Disciplines ◽  
Wide Range

The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

β-Casein monomers as potential flavonoids nanocarriers: Thermodynamics and kinetics of β-casein-naringin binding by fluorescence spectroscopy and surface plasmon resonance

2020 ◽  
Vol 108 ◽  
pp. 104728
Author(s):  
Ana Flávia Coelho Pacheco ◽  
Natália Moreira Nunes ◽  
Hauster Maximiler Campos de Paula ◽  
Yara Luiza Coelho ◽  
Luis Henrique Mendes da Silva ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Thermodynamics And Kinetics ◽  
Kinetics Of

scholarly journals Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cdna Probe ◽  
Dna Aptamer ◽  
Dopamine Detection ◽  
Calibration Curves ◽  
20 Nm

ABSTRACTUltrasensitive and selective detection and quantification of dopamine (DA) plays a key role in monitoring neurodegenerative diseases. However, the detection limit reported for DA detection is typically in the lower nM range. Pushing the detection limit to pM or lower for this particular target to cover the physiological levels (< 130 pM) is significant. Herein, DA DNA aptamer (DAAPT) gold nanoparticle (AuNP) conjugate is utilized to enhance the surface plasmon resonance (SPR) signal, which enables to detect and quantify DA in the femtomolar (200 fM) to picomolar range. To the best of our knowledge, this is the lowest detection limit achieved for SPR sensing of dopamine. The as-prepared 10 nm DAAPT-AuNP conjugate demonstrates strong binding affinity (Kd = 3.1 ± 1.4 nM) to the complementary DNA (cDNA) probe on gold chip. The cDNA probe is immobilized to the chip via polydopamine surface chemistry, which allows the Michael addition of any primary amine-terminated biomolecules. By adjusting the concentration of the DAAPT-AuNP conjugate, two calibration curves are generated with dynamic ranges from 100 µM to 2 mM, and from 200 fM to 20 nM, respectively. Both calibration curves have negative slopes, showing good agreement to a dose-response curve in an enzyme inhibition assay. In addition, the sensing strategy is evaluated to be specific for DA detection using a series of DA analogs and other metabolites as potential interferences.

Thermodynamic and kinetic study of epigallocatechin-3-gallate-bovine lactoferrin complex formation determined by surface plasmon resonance (SPR): A comparative study with fluorescence spectroscopy

2019 ◽  
Vol 95 ◽  
pp. 526-532 ◽  
Author(s):  
Jaqueline de Paula Rezende ◽  
Eliara Acipreste Hudson ◽  
Hauster Maximiler Campos de Paula ◽  
Yara Luiza Coelho ◽  
Luis Henrique Mendes da Silva ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Complex Formation ◽  
Fluorescence Spectroscopy ◽  
Comparative Study ◽  
Kinetic Study ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bovine Lactoferrin

A Hydride Generation-Nanosilver Surface Plasmon Resonance Absorption Spectral Method for the Determination of Trace Se(IV)

2013 ◽  
Vol 749 ◽  
pp. 491-494
Author(s):  
Ai Hui Liang ◽  
Qing Ye Liu ◽  
Gui Qing Wen ◽  
Ting Sheng Li ◽  
Zhi Liang Jiang
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Water Samples ◽  
Hydride Generation ◽  
Resonance Absorption ◽  
Surface Plasmon Resonance Absorption ◽  
Hcl Solution

In HCl solution, the Se (IV) was reduced to SeH2 by NaBH4, and absorbed by solution of ethanol-AgNO3. The Ag+ was reduced to nanosilver that exhibited surface plasmon resonance absorption (SPR) peaks at 292 nm and 420 nm. Under the selected conditions, the value at 292 nm was linear to the concentration of Se (IV) in the range of 0.08-2.0 μg/mL, a detection limit of 0.04 μg/mL. The proposed method was applied to detect Se (IV) in water samples, with satisfactory results.

scholarly journals Determination of Vitamin B12 in Fortified Bovine Milk- Based Infant Formula Powder, Fortified Soya-Based Infant Formula Powder, Vitamin Premix, and Dietary Supplements by Surface Plasmon Resonance: Collaborative Study

2011 ◽  
Vol 94 (4) ◽  
pp. 1217-1226 ◽  
Author(s):  
Pathik Vyas ◽  
Anthony A O'kane ◽  
E Ager ◽  
S Crooks ◽  
C Elliott ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Protein Binding ◽  
Vitamin B12 ◽  
Infant Formula ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Protein ◽  
Collaborative Study ◽  
Wide Range

Abstract A collaborative study was conducted on an inhibition-based protein-binding assay using the Biacore Q™ biosensor instrument and the Biacore Qflex™ Kit Vitamin B12 PI. The samples studied included infant formula, cereals, premixes, vitamin tablets, dietary supplements, and baby food. The collaborative study, which involved 11 laboratories, demonstrated that the assay showed an RSDr of 1.59–27.8 and HorRat values for reproducibility of 0.34–1.89 in samples with levels ranging from ppm to ppb. The assay studied is a label-free protein binding-based assay that uses the principle of surface plasmon resonance (SPR) to measure the interaction between vitamin B12 and a specifc binding protein. A Biacore Q biosensor uses this principle to detect binding directly at the surface of a sensor chip with a hydrophilic gold-dextran surface. The instrument passes a mixture of prepared sample extract and binding protein solution across a covalently immobilized vitamin B12 chip surface, and the response is given as free-binding protein as the mixture binds to the immobilized surface. This technique uses the specifcity and robustness of the protein-ligand interaction to allow minimal sample preparation and a wide range of matrixes to be analyzed rapidly. The reagents and accessories needed to perform this assay are provided as the ready-to-use format “Qflex Kit Vitamin B12 PI.” The method is intended for routine use in the quantitative determination of vitamin B12 (as cyanocobalamin) in a wide range of food products, dietary vitamin supplements, and multivitamin premixes.

Rapid surface plasmon resonance immunoassays for the determination of mycotoxins in cereals and cereal-based food products

2014 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
J.P. Meneely ◽  
C.T. Elliott
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cost Effective ◽  
Food Products ◽  
Future Research ◽  
Portable Devices ◽  
Wide Range ◽  
Food And Feed ◽  
Robust Sensing

In recent times surface plasmon resonance has demonstrated its applicability to the detection of a wide range of contaminants in food and feed including mycotoxins in cereals and cereal-based food products. Commercially available, laboratory-based systems have exploited high affinity polyclonal, monoclonal and recombinant antibodies and robust sensing surfaces to provide rapid, accurate and sensitive means of determining these toxins. In addition many custom-built, prototype devices have shown a great deal of potential for this particular application and have included the combination of surface plasmon resonance with enzyme-derivatised sensors, molecularly imprinted polymers, fluorescence spectroscopy and the use of gold nanoparticles for signal enhancement. Of note is the lack of available devices that allow the detection of multiple mycotoxins simultaneously and portable devices that could be used in the field, therefore future research and development should focus on these areas to deliver cost-effective miniaturised devices with multiplexing capabilities.

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