Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

2006 ◽  
Vol 951 ◽  
Author(s):  
Praveen Singh ◽  
Takeshi Onodera ◽  
Kiyoshi Matsumoto ◽  
Norio Miura ◽  
Kiyoshi Toko
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Self Assembly ◽  
Competitive Inhibition ◽  
Layer By Layer ◽  
Self Assembled Monolayer ◽  
Chip Surface ◽  
Resonance Sensor

ABSTRACTA nano-scale biosensor chip surface was fabricated using dinitro-phenylated key hole limpet (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self assembled monolayer (SAM) and bis sulfosuccinimidyl suberate(BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml(140 ppt) for TNT molecule. The detection limit of surface plasmon resonance(SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format. A short injection (12 sec) of 10 mM Glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

Surface plasmon resonance sensor using functionalized alkanethiols monolayer for illegal compound detection

2016 ◽  
Vol 25 (01) ◽  
pp. 1650007 ◽  
Author(s):  
Suherman ◽  
Kinichi Morita ◽  
Toshikazu Kawaguchi
Keyword(s):  
Surface Plasmon Resonance ◽  
Molecular Electronics ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Competitive Inhibition ◽  
Coupling Reaction ◽  
Gold Surface ◽  
Sensor Surface ◽  
Resonance Sensor ◽  
Highly Sensitive Detection

The development of highly sensitive detection of [Formula: see text]-agonists compound by using surface plasmon resonance (SPR) immunoassay functionalized alkanethiols monolayer on gold surface was reported here. For the construction of robust SPR sensor surface, the illegal compound (as antigen) was immobilized onto gold succinimide-terminated monolayer to perform amide-coupling reaction. Structural characteristic of monolayer is an important issue for further application of selt-assembled monolayer (SAM)-based molecular electronics. This study focuses on the relation between structure of alkanethiols SAM of different chain length and its sensitivity of the detection. The surface structure is characterized by electrochemical methods and STM. The target compound in this research is clenbuterol as farmers used illegally to increase their profit gain. For the detection of clenbuterol, indirect competitive inhibition method was used. In this work, the SPR senses the dielectric constant change at the interface from the binding of the unreacted antibody to antigen-immobilized on the sensor surface. By this method, clenbuterol can be detected within ppt level and only requires 5[Formula: see text]min per sample. Furthermore, shorter chain alkanethiol sensor surface produces higher sensitivity in the detection process.

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