Injectable oxygen-generating nanocomposite hydrogels with prolonged oxygen delivery for enhanced cell proliferation under hypoxic and normoxic conditions

2020 ◽  
Vol 8 (19) ◽  
pp. 4195-4201 ◽  
Author(s):  
Andisheh Motealleh ◽  
Nermin S. Kehr
Keyword(s):  
Cell Proliferation ◽  
Oxygen Delivery ◽  
Organic Peroxide ◽  
Fibroblast Cells ◽  
Malignant Cells ◽  
Nanocomposite Hydrogels ◽  
Biomaterial Scaffold

We describe a new organic peroxide-based injectable biomaterial that provides sustained O2 within the 3D biomaterial scaffold and so enhances the viability of healthy fibroblast cells, while reduces the proliferation of malignant cells.

scholarly journals Nanoprodrugs of NSAIDs Inhibit the Growth of U87-MG Glioma Cells

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Bong-Seop Lee ◽  
Xiangpeng Yuan ◽  
Qijin Xu ◽  
Minhee K. Ko ◽  
Aruna K. Nalla ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Anticancer Agents ◽  
Glioma Cells ◽  
Cell Counting ◽  
Malignant Cells ◽  
Spontaneous Emulsification ◽  
Superior Effect ◽  
Inflammatory Drugs ◽  
Derivatives Of

Several recent reports have demonstrated that nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit the growth of various malignant cells suggesting their application as anticancer agents. In this study, we prepared six nanometer-sized prodrugs (nanoprodrugs) of NSAIDs, ibuprofen, indomethacin, and naproxen through the spontaneous emulsification mechanism using monomeric and dimeric derivatives of the NSAIDs. We evaluated their effect on the proliferation of U87-MG glioma cells by cell counting, WST-1 cell proliferation reagent, and propidium iodide incorporation. The two ibuprofen nanoprodrugs inhibited the cell growth more potently than the indomethacin nanoprodrugs, whereas the naproxen nanoprodrugs did not show any significant effect. Remarkably, ibuprofen did not show any effect at an equimolar concentration. Approximately, 4.4% of the ibuprofen nanoprodrugs was found in the cell, whereas no ibuprofen could be detected suggesting that the superior effect of the nanoprodrugs can be attributed to the efficient cellular uptake of the nanoprodrugs.

scholarly journals Elucidation of Antimicrobial Silver Sulfadiazine (SSD) Blend/Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) Immobilised with Collagen Peptide as Potential Biomaterial

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2979
Author(s):  
Sevakumaran Vigneswari ◽  
Tana Poorani Gurusamy ◽  
H. P. S. Abdul Khalil ◽  
Seeram Ramakrishna ◽  
Al-Ashraf Abdullah Amirul
Keyword(s):  
Cell Proliferation ◽  
Tissue Regeneration ◽  
Medical Application ◽  
Silver Sulfadiazine ◽  
Tissue Response ◽  
Fibroblast Cells ◽  
Gram Positive Bacteria ◽  
Collagen Peptide ◽  
L929 Fibroblast ◽  
Antimicrobial Performance

The quest for a suitable biomaterial for medical application and tissue regeneration has resulted in the extensive research of surface functionalization of material. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial polymer well-known for its high levels of biocompatibility, non-genotoxicity, and minimal tissue response. We have designed a porous antimicrobial silver SSD blend/poly(3HB-co-4HB)-collagen peptide scaffold using a combination of simple techniques to develop a scaffold with an inter-connected microporous pore in this study. The collagen peptide was immobilised via -NH2 group via aminolysis. In order to improve the antimicrobial performance of the scaffold, silver sulfadiazine (SSD) was impregnated in the scaffolds. To confirm the immobilised collagen peptide and SSD, the scaffold was characterized using FTIR. Herein, based on the cell proliferation assay of the L929 fibroblast cells, enhanced bioactivity of the scaffold with improved wettability facilitated increased cell proliferation. The antimicrobial activity of the SSD blend/P(3HB-co-4HB)-collagen peptide in reference to the pathogenic Gram-negative, Gram-positive bacteria and yeast Candida albicans exhibited SSD blend/poly(3HB-co-4HB)-12.5 wt% collagen peptide as significant construct of biocompatible antibacterial biomaterials. Thus, SSD blend/P(3HB-co-4HB)-collagen peptide scaffold from this finding has high potential to be further developed as biomaterial.

scholarly journals The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

2019 ◽  
Vol 18 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Gulsah Gundogdu ◽  
Koksal Gundogdu ◽  
Kemal Alp Nalci ◽  
Alper Kursat Demirkaya ◽  
Seymanur Yılmaz Tascı ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Dose Range ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells ◽  
Wound Model ◽  
Dpph Method ◽  
Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

Biphasic effect of cadmium on cell proliferation in human embryo lung fibroblast cells and its molecular mechanism

2009 ◽  
Vol 23 (6) ◽  
pp. 973-978 ◽  
Author(s):  
Gaofeng Jiang ◽  
Weixia Duan ◽  
Lei Xu ◽  
Shizhen Song ◽  
Changcai Zhu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Molecular Mechanism ◽  
Human Embryo ◽  
Lung Fibroblast ◽  
Fibroblast Cells ◽  
Biphasic Effect

scholarly journals A laktátdehidrogenáz (LDH) prognosztikai jelentősége az onkológiában

2017 ◽  
Vol 158 (50) ◽  
pp. 1977-1988 ◽  
Author(s):  
Dániel Deme ◽  
András Telekes
Keyword(s):  
Cell Proliferation ◽  
Lactate Dehydrogenase ◽  
Immune Responses ◽  
Malignant Cells ◽  
Malignant Diseases ◽  
Adaptive Immune Responses ◽  
Reversible Process ◽  
Baseline Serum ◽  
Adaptive Immune ◽  
Lactate Formation

Abstract: Glycolysis is increased in most of the malignant cells, providing the largest proportion of energy needed for cell proliferation. Lactate dehydrogenase (LDH) catalyses the reversible process of pyruvate to lactate in anaerobic condition. LDHA isoenzyme expressed mainly by malignant cells, significantly increases lactate formation. Lactate induces the proliferation of oxygenated malignant cells, angiogenesis, and inhibits the innate and adaptive immune responses. Baseline serum LDH elevation correlates with shorter survival. The authors review the relevant studies exploring the correlation between LDH elevation and the prognosis of malignant diseases. Orv Hetil. 2017; 158(50): 1977–1988.

Cell proliferation and cell sheet detachment from the positively and negatively charged nanocomposite hydrogels

Biopolymers ◽  
2013 ◽  
Vol 101 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Dan Liu ◽  
Tao Wang ◽  
Xinxing Liu ◽  
Zhen Tong
Keyword(s):  
Cell Proliferation ◽  
Cell Sheet ◽  
Nanocomposite Hydrogels
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