Cell proliferation and cell sheet detachment from the positively and negatively charged nanocomposite hydrogels

Biopolymers ◽  
2013 ◽  
Vol 101 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Dan Liu ◽  
Tao Wang ◽  
Xinxing Liu ◽  
Zhen Tong
Keyword(s):  
Cell Proliferation ◽  
Cell Sheet ◽  
Nanocomposite Hydrogels

Silk sericin/poly (NIPAM/LMSH) nanocomposite hydrogels: Rapid thermo-responsibility and good carrier for cell proliferation

2014 ◽  
Vol 86 (5) ◽  
pp. 721-731 ◽  
Author(s):  
Qingsong Zhang ◽  
Rui Xue ◽  
Xuewei Li ◽  
Zheng Wang ◽  
Zhiyi Zhou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Activity ◽  
L929 Cell ◽  
Magnesium Silicate ◽  
Enzymatic Digestion ◽  
L929 Cells ◽  
Silk Sericin ◽  
Cell Compatibility ◽  
Nanocomposite Hydrogels ◽  
Cross Linker

AbstractTo overcome the fragility of chemical cross-linked hydrogel and improve the cell compatibility of poly(N-isopropylacrylamide) hydrogel, physical cross-linker lithium magnesium silicate hydrate (LMSH) and silk sericin (SS) created by Bombyx mori (silkworms) were adopted as physical cross-linker and biocompatible monomer to prepare SS/poly(NIPAM/LMSH) nanocomposite hydrogels. The temperature sensitivity and cytocompatibility like mouse fibroblasts (L929) cell proliferation and desorption were investigated. Depending on SS content, the resultant nanocomposite hydrogels present bigger volume and rapid thermo-responsibility. The volume-phase transmittance temperature (VPTT) of SS/poly(NIPAM/LMSH) nanocomposite hydrogels increases from 32.12 to 33.34 °C with the introduction of SS content. Compared to pure poly(NIPAM/LMSH) nanocomposite hydrogel, MTT and SEM graphs have proved that the incorporation of SS could accelerate cell adhesion, spreading and detachment of L929 cells on the surface of SS/poly(NIPAM/LMSH) nanocomposite hydrogels. On culturing 5d, L929 cells have covered the whole surface of SS/poly(NIPAM/LMSH) nanocomposite hydrogels. By reducing temperature from 37 to 15 °C, L929 cells on the surface of SS/poly(NIPAM/LMSH) nanocomposite hydrogels detach spontaneously and harvested L929 cells still keep better cell activity than that via enzymatic digestion.

Injectable oxygen-generating nanocomposite hydrogels with prolonged oxygen delivery for enhanced cell proliferation under hypoxic and normoxic conditions

2020 ◽  
Vol 8 (19) ◽  
pp. 4195-4201 ◽  
Author(s):  
Andisheh Motealleh ◽  
Nermin S. Kehr
Keyword(s):  
Cell Proliferation ◽  
Oxygen Delivery ◽  
Organic Peroxide ◽  
Fibroblast Cells ◽  
Malignant Cells ◽  
Nanocomposite Hydrogels ◽  
Biomaterial Scaffold

We describe a new organic peroxide-based injectable biomaterial that provides sustained O2 within the 3D biomaterial scaffold and so enhances the viability of healthy fibroblast cells, while reduces the proliferation of malignant cells.

scholarly journals Effect of Gelatin on Osteogenic Cell Sheet Formation Using Canine Adipose-Derived Mesenchymal Stem Cells

2017 ◽  
Vol 26 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Ah Young Kim ◽  
Yongsun Kim ◽  
Seung Hoon Lee ◽  
Yongseok Yoon ◽  
Wan-Hee Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Cell Sheet ◽  
Proliferation Rate ◽  
Osteogenic Cell ◽  
Cell Sheets ◽  
Gene Markers ◽  
Cell Proliferation Rate

Osteogenically differentiated cell sheet techniques using mesenchymal stem cells (MSCs) are available to stimulate bone regeneration. The advantage of the cell sheet technique is delivering live cells effectively into the focal region. We developed a novel osteogenic cell sheet technique by adding gelatin to osteogenic cell medium. Gelatin-induced osteogenic cell sheets (GCSs) were compared to conventional osteogenic cell sheets (OCSs). Undifferentiated MSCs (UCs) were used as a control. The morphology of these cell sheets was evaluated microscopically and histologically. The time-dependent cell proliferation rate was estimated by DNA quantification. The expression of osteogenic gene markers and the number of calcium depositions were assessed by quantitative real-time polymerase chain reaction and Alizarin red S (ARS) staining, respectively. GCSs were thicker and stronger than OCSs. GCSs showed a significantly higher cell proliferation rate compared to OCSs ( p < 0.05). GCSs exhibited significantly higher upregulation of BMP-7 mRNA compared to OCSs ( p < 0.05). Both GCSs and OCSs showed negative ARS reactivity on day 10, but only GCSs showed positive ARS reactivity on day 21. With this technique, we observed active cell proliferation with abundant ECM and upregulation of osteogenic bone markers, and our results suggest that GCSs could be promising for therapeutic applications in bone regeneration.

Rapid cell sheet detachment from alginate semi-interpenetrating nanocomposite hydrogels of PNIPAm and hectorite clay

2011 ◽  
Vol 71 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Tao Wang ◽  
Dan Liu ◽  
Cuixia Lian ◽  
Shudian Zheng ◽  
Xinxing Liu ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Nanocomposite Hydrogels
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