scholarly journals Elucidation of Antimicrobial Silver Sulfadiazine (SSD) Blend/Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) Immobilised with Collagen Peptide as Potential Biomaterial

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2979
Author(s):  
Sevakumaran Vigneswari ◽  
Tana Poorani Gurusamy ◽  
H. P. S. Abdul Khalil ◽  
Seeram Ramakrishna ◽  
Al-Ashraf Abdullah Amirul
Keyword(s):  
Cell Proliferation ◽  
Tissue Regeneration ◽  
Medical Application ◽  
Silver Sulfadiazine ◽  
Tissue Response ◽  
Fibroblast Cells ◽  
Gram Positive Bacteria ◽  
Collagen Peptide ◽  
L929 Fibroblast ◽  
Antimicrobial Performance

The quest for a suitable biomaterial for medical application and tissue regeneration has resulted in the extensive research of surface functionalization of material. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial polymer well-known for its high levels of biocompatibility, non-genotoxicity, and minimal tissue response. We have designed a porous antimicrobial silver SSD blend/poly(3HB-co-4HB)-collagen peptide scaffold using a combination of simple techniques to develop a scaffold with an inter-connected microporous pore in this study. The collagen peptide was immobilised via -NH2 group via aminolysis. In order to improve the antimicrobial performance of the scaffold, silver sulfadiazine (SSD) was impregnated in the scaffolds. To confirm the immobilised collagen peptide and SSD, the scaffold was characterized using FTIR. Herein, based on the cell proliferation assay of the L929 fibroblast cells, enhanced bioactivity of the scaffold with improved wettability facilitated increased cell proliferation. The antimicrobial activity of the SSD blend/P(3HB-co-4HB)-collagen peptide in reference to the pathogenic Gram-negative, Gram-positive bacteria and yeast Candida albicans exhibited SSD blend/poly(3HB-co-4HB)-12.5 wt% collagen peptide as significant construct of biocompatible antibacterial biomaterials. Thus, SSD blend/P(3HB-co-4HB)-collagen peptide scaffold from this finding has high potential to be further developed as biomaterial.

scholarly journals Surface Characterization and Physiochemical Evaluation of P(3HB-co-4HB)-Collagen Peptide Scaffolds with Silver Sulfadiazine as Antimicrobial Agent for Potential Infection-Resistance Biomaterial

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2454
Author(s):  
Sevakumaran Vigneswari ◽  
Tana Poorani Gurusamy ◽  
Wan M. Khairul ◽  
Abdul Khalil H.P.S. ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Controlled Release ◽  
Antimicrobial Agent ◽  
Surface Characterization ◽  
Biomedical Application ◽  
Physical And Mechanical Properties ◽  
Silver Sulfadiazine ◽  
Salt Leaching ◽  
Collagen Peptides ◽  
Collagen Peptide ◽  
Peptide Scaffolds

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial derived biopolymer widely known for its unique physical and mechanical properties to be used in biomedical application. In this study, antimicrobial agent silver sulfadiazine (SSD) coat/collagen peptide coat-P(3HB-co-4HB) (SCCC) and SSD blend/collagen peptide coat-P(3HB-co-4HB) scaffolds (SBCC) were fabricated using a green salt leaching technique combined with freeze-drying. This was then followed by the incorporation of collagen peptides at various concentrations (2.5–12.5 wt.%) to P(3HB-co-4HB) using collagen-coating. As a result, two types of P(3HB-co-4HB) scaffolds were fabricated, including SCCC and SBCC scaffolds. The increasing concentrations of collagen peptides from 2.5 wt.% to 12.5 wt.% exhibited a decline in their porosity. The wettability and hydrophilicity increased as the concentration of collagen peptides in the scaffolds increased. In terms of the cytotoxic results, MTS assay demonstrated the L929 fibroblast scaffolds adhered well to the fabricated scaffolds. The 10 wt.% collagen peptides coated SCCC and SBCC scaffolds displayed highest cell proliferation rate. The antimicrobial analysis of the fabricated scaffolds exhibited 100% inhibition towards various pathogenic microorganisms. However, the SCCC scaffold exhibited 100% inhibition between 12 and 24 h, but the SBCC scaffolds with SSD impregnated in the scaffold had controlled release of the antimicrobial agent. Thus, this study will elucidate the surface interface-cell interactions of the SSD-P(3HB-co-4HB)-collagen peptide scaffolds and controlled release of SSD, antimicrobial agent.

Cytocompatibility property evaluation of gas pressure sintered SiAlON–SiC composites with L929 fibroblast cells and Saos-2 osteoblast-like cells

2012 ◽  
Vol 32 (3) ◽  
pp. 464-469 ◽  
Author(s):  
Alok Kumar ◽  
Awadesh Kumar Mallik ◽  
Nurcan Calis Acikbas ◽  
Merve Yaygıngol ◽  
Ferhat Kara ◽  
...  
Keyword(s):  
Gas Pressure ◽  
Fibroblast Cells ◽  
Property Evaluation ◽  
L929 Fibroblast ◽  
Sic Composites

Cytotoxicity of HC Orange NO. 1 to L929 fibroblast cells

2008 ◽  
Vol 26 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Hongling Liu ◽  
Jiangning Chen ◽  
Jie Jiang ◽  
John P. Giesy ◽  
Hongxia Yu ◽  
...  
Keyword(s):  
Fibroblast Cells ◽  
L929 Fibroblast

scholarly journals Hypoxia in Cell Reprogramming and the Epigenetic Regulations

2021 ◽  
Vol 9 ◽  
Author(s):  
Nariaki Nakamura ◽  
Xiaobing Shi ◽  
Radbod Darabi ◽  
Yong Li
Keyword(s):  
Cell Proliferation ◽  
Cell Fate ◽  
Tissue Regeneration ◽  
Cellular Reprogramming ◽  
Cell Reprogramming ◽  
Cell Renewal ◽  
Cellular Functions ◽  
Stem Cell Renewal ◽  
Epigenetic Regulations

Cellular reprogramming is a fundamental topic in the research of stem cells and molecular biology. It is widely investigated and its understanding is crucial for learning about different aspects of development such as cell proliferation, determination of cell fate and stem cell renewal. Other factors involved during development include hypoxia and epigenetics, which play major roles in the development of tissues and organs. This review will discuss the involvement of hypoxia and epigenetics in the regulation of cellular reprogramming and how interplay between each factor can contribute to different cellular functions as well as tissue regeneration.

scholarly journals The effect of substrate pore size on the differentiation of L929 fibroblast cells: Morphological observations and expression of type 1 collagen mRNA

2004 ◽  
Vol 25 (2) ◽  
pp. 61-68 ◽  
Author(s):  
Tatsuo SHIIGAI ◽  
Masaki SHIMONO ◽  
Masao YOSHINARI ◽  
Kenichi MATSUZAKA ◽  
Takashi INOUE
Keyword(s):  
Pore Size ◽  
Fibroblast Cells ◽  
Collagen Mrna ◽  
L929 Fibroblast

scholarly journals The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

2019 ◽  
Vol 18 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Gulsah Gundogdu ◽  
Koksal Gundogdu ◽  
Kemal Alp Nalci ◽  
Alper Kursat Demirkaya ◽  
Seymanur Yılmaz Tascı ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Dose Range ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells ◽  
Wound Model ◽  
Dpph Method ◽  
Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

Analysis of Cell Viability and Gene Expression After Continuous Ultrasound Therapy in L929 Fibroblast Cells

2019 ◽  
Vol 98 (5) ◽  
pp. 369-372 ◽  
Author(s):  
Larissa Dragonetti Bertin ◽  
Regina Célia Poli-Frederico ◽  
Deise Aparecida Almeida Pires Oliveira ◽  
Priscila Daniele Oliveira ◽  
Flávia Beltrão Pires ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Ultrasound Therapy ◽  
Fibroblast Cells ◽  
L929 Fibroblast

scholarly journals Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lingling Jiang ◽  
Boyang Liu ◽  
Yan Qi ◽  
Linru Zhu ◽  
Xueling Cui ◽  
...  
Keyword(s):  
Mapk Pathway ◽  
Activin A ◽  
Fibroblast Cells ◽  
L929 Cells ◽  
Tissue Fibrosis ◽  
Antagonistic Effects ◽  
Tnf Α ◽  
L929 Fibroblast ◽  
Relationship Of ◽  
Tgf Β1

AbstractFibroblasts play an important role in inflammation and tissue fibrosis. Both activin A and TNF-α can activate immune cells, however, the roles and relationship of them in activating fibroblasts in inflammation remain unclear. Here, this study revealed that TNF-α promoted the release of NO and IL-6 by L929 fibroblast cells, but co-treatment with activin A attenuated these effects. In contrast, activin A induced cell migration and increased the production of tissue fibrosis-related TGF-β1 and fibronectin, while TNF-α inhibited these function changes of L929 cells induced by activin A. Moreover, this study revealed that activin A and TNF-α regulated the activities of L929 cells via ERK1/2/MAPK pathway, rather than Smad3-dependent signaling pathway. Taken together, these data indicate that activin A and TNF-α exert mutually antagonistic effects on regulating fibroblasts activities, and the balance between their action may determine the process and outcome of fibroblasts-mediated inflammation.

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