scholarly journals Twisting and tilting of a mechanosensitive molecular probe detects order in membranes

2020 ◽  
Vol 11 (22) ◽  
pp. 5637-5649 ◽  
Author(s):  
Giuseppe Licari ◽  
Karolina Strakova ◽  
Stefan Matile ◽  
Emad Tajkhorshid
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe ◽  
Membrane Tension ◽  
Lipid Packing

Flipper-TR fluorescent probe detects lipid packing and membrane tension by twisting its mechanosensitive flippers and by changing its orientation in the membrane.

scholarly journals A “Double-Locked” and enzyme-activated molecular probe for accurate bioimaging and hepatopathy differentiation

2019 ◽  
Vol 10 (47) ◽  
pp. 10931-10936 ◽  
Author(s):  
Yongchao Liu ◽  
Lili Teng ◽  
Chengyan Xu ◽  
Hong-Wen Liu ◽  
Shuai Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe

A “double-locked” and enzyme-activated molecular fluorescent probe for accurate bioimaging and hepatopathy differentiation.

scholarly journals Imaging non-classical mechanical responses of lipid membranes using molecular rotors

2021 ◽  
Author(s):  
Miguel Paez-Perez ◽  
ismael lópez-duarte ◽  
Aurimas Vysniauskas ◽  
Nicholas Jan Brooks ◽  
Marina Konstantinovna Kuimova
Keyword(s):  
Direct Effect ◽  
Lipid Membranes ◽  
Molecular Rotors ◽  
Membrane Tension ◽  
Cellular Membranes ◽  
Cellular Adaptation ◽  
Lipid Packing ◽  
Mechanical Responses

Lipid packing in cellular membranes has a direct effect on membrane tension and microviscosity, and plays a central role in cellular adaptation, homeostasis and disease. According to conventional mechanical descriptions,...

A highly selective ratiometric molecular probe for imaging peroxynitrite during drug-induced acute liver injury

2021 ◽  
Vol 9 (39) ◽  
pp. 8246-8252
Author(s):  
Xiangyang Gong ◽  
Dan Cheng ◽  
Wei Li ◽  
Yang Shen ◽  
Rong Peng ◽  
...  
Keyword(s):  
Liver Injury ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Acute Liver Injury ◽  
Molecular Hybridization ◽  
Molecular Probe ◽  
Drug Induced ◽  
Ratiometric Fluorescent Probe ◽  
Complex Drug

Visualizing ONOO− fluctuations in the complex drug-induced acute liver injury process using a ratiometric fluorescent probe developed by using a molecular hybridization strategy with high selectivity and sensitivity.

scholarly journals Fluorescent Probe for the Imaging of Superoxide and Peroxynitrite during Drug-induced Liver Injury

2021 ◽  
Author(s):  
Luling Wu ◽  
Jihong Liu ◽  
Xue Tian ◽  
Robin Rupert Groleau ◽  
Steven Bull ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Injury ◽  
Fluorescent Probe ◽  
Molecular Probe ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Fatal Liver

Drug-induced liver injury (DILI) is an important cause of potentially fatal liver disease. Herein, we report the development of a molecular probe (LW−OTf) for the detection and imaging of two...

A new mitochondrion targetable fluorescent probe for carbon monoxide-specific detection and live cell imaging

2019 ◽  
Vol 55 (64) ◽  
pp. 9444-9447 ◽  
Author(s):  
Chenxia Zhang ◽  
Hai Xie ◽  
Tongxia Zhan ◽  
Jie Zhang ◽  
Bochao Chen ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Biological Systems ◽  
Live Cell ◽  
Molecular Probe ◽  
Powerful Method ◽  
Specific Detection

A red molecular probe for carbon monoxide (CO)-specific detection based on palladium-free mediated opening of spirolactam has been applied to establish a safe and powerful method to detect and image CO changes in biological systems.

Feedback between membrane tension, lipid shape and curvature in the formation of packing defects

2018 ◽  
Author(s):  
M. Pinot ◽  
S. Vanni ◽  
E. Ambroggio ◽  
D. Guet ◽  
B. Goud ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Lipid Membrane ◽  
Membrane Curvature ◽  
Membrane Tension ◽  
Membrane Pore ◽  
Membrane Deformation ◽  
Lipid Packing ◽  
Head Groups ◽  
Packing Defects ◽  
The Alps

AbstractLipid packing defects favor the binding of proteins to cellular membranes by creating spaces between lipid head groups that allow the insertion of amphipathic helices or lipid modifications. The density of packing defects in a lipid membrane is well known to increase with membrane curvature and in the presence of conical-shaped lipids. In contrast, the role of membrane tension in the formation of lipid packing defects has been poorly investigated. Here we use a combination of numerical simulations and experiments to measure the effect of membrane tension on the density of lipid packing defects. We first monitor the binding of ALPS (amphipathic lipid packing sensor) to giant unilamellar vesicles and observe a striking periodic binding of ALPS that we attribute to osmotically-induced membrane tension and transient membrane pore formation. Using micropipette aspiration experiments, we show that a high membrane tension induces a reversible increase in the density of lipid packing defects. We next focus on packing defects induced by lipid shape and show that conical lipids generate packing defects similar to that induced by membrane tension and enhance membrane deformation due to the insertion of the ALPS helix. Both cyclic ALPS binding and the cooperative effect of ALPS binding and conical lipids on membrane deformation result from an interplay between helix insertion and lipid packing defects created by membrane tension, conical lipids and/or membrane curvature. We propose that feedback mechanisms involving membrane tension, lipid shape and membrane curvature play a crucial role in membrane deformation and intracellular transport events.

scholarly journals Penta-fluorophenol: a Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma

2020 ◽  
Vol 11 (22) ◽  
pp. 5658-5668 ◽  
Author(s):  
Jong Min An ◽  
Sangrim Kang ◽  
Eugene Huh ◽  
Yejin Kim ◽  
Dahae Lee ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe ◽  
Smiles Rearrangement ◽  
Human Glioblastoma

A fluorescent molecular probe for the identification of glioblastoma is developed. The probe allows the tracing of the cysteine (Cys) level, which is recognized as a new biomarker of GBM.

Merocyanine 540, a fluorescent probe sensitive to lipid packing

1983 ◽  
Vol 732 (2) ◽  
pp. 387-393 ◽  
Author(s):  
Patrick Williamson ◽  
Karen Mattocks ◽  
Robert A. Schlegel
Keyword(s):  
Fluorescent Probe ◽  
Lipid Packing ◽  
Merocyanine 540
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