scholarly journals A “Double-Locked” and enzyme-activated molecular probe for accurate bioimaging and hepatopathy differentiation

2019 ◽  
Vol 10 (47) ◽  
pp. 10931-10936 ◽  
Author(s):  
Yongchao Liu ◽  
Lili Teng ◽  
Chengyan Xu ◽  
Hong-Wen Liu ◽  
Shuai Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe

A “double-locked” and enzyme-activated molecular fluorescent probe for accurate bioimaging and hepatopathy differentiation.

scholarly journals Twisting and tilting of a mechanosensitive molecular probe detects order in membranes

2020 ◽  
Vol 11 (22) ◽  
pp. 5637-5649 ◽  
Author(s):  
Giuseppe Licari ◽  
Karolina Strakova ◽  
Stefan Matile ◽  
Emad Tajkhorshid
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe ◽  
Membrane Tension ◽  
Lipid Packing

Flipper-TR fluorescent probe detects lipid packing and membrane tension by twisting its mechanosensitive flippers and by changing its orientation in the membrane.

A highly selective ratiometric molecular probe for imaging peroxynitrite during drug-induced acute liver injury

2021 ◽  
Vol 9 (39) ◽  
pp. 8246-8252
Author(s):  
Xiangyang Gong ◽  
Dan Cheng ◽  
Wei Li ◽  
Yang Shen ◽  
Rong Peng ◽  
...  
Keyword(s):  
Liver Injury ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Acute Liver Injury ◽  
Molecular Hybridization ◽  
Molecular Probe ◽  
Drug Induced ◽  
Ratiometric Fluorescent Probe ◽  
Complex Drug

Visualizing ONOO− fluctuations in the complex drug-induced acute liver injury process using a ratiometric fluorescent probe developed by using a molecular hybridization strategy with high selectivity and sensitivity.

scholarly journals Fluorescent Probe for the Imaging of Superoxide and Peroxynitrite during Drug-induced Liver Injury

2021 ◽  
Author(s):  
Luling Wu ◽  
Jihong Liu ◽  
Xue Tian ◽  
Robin Rupert Groleau ◽  
Steven Bull ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Injury ◽  
Fluorescent Probe ◽  
Molecular Probe ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Fatal Liver

Drug-induced liver injury (DILI) is an important cause of potentially fatal liver disease. Herein, we report the development of a molecular probe (LW−OTf) for the detection and imaging of two...

A new mitochondrion targetable fluorescent probe for carbon monoxide-specific detection and live cell imaging

2019 ◽  
Vol 55 (64) ◽  
pp. 9444-9447 ◽  
Author(s):  
Chenxia Zhang ◽  
Hai Xie ◽  
Tongxia Zhan ◽  
Jie Zhang ◽  
Bochao Chen ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Biological Systems ◽  
Live Cell ◽  
Molecular Probe ◽  
Powerful Method ◽  
Specific Detection

A red molecular probe for carbon monoxide (CO)-specific detection based on palladium-free mediated opening of spirolactam has been applied to establish a safe and powerful method to detect and image CO changes in biological systems.

scholarly journals Penta-fluorophenol: a Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma

2020 ◽  
Vol 11 (22) ◽  
pp. 5658-5668 ◽  
Author(s):  
Jong Min An ◽  
Sangrim Kang ◽  
Eugene Huh ◽  
Yejin Kim ◽  
Dahae Lee ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Molecular Probe ◽  
Smiles Rearrangement ◽  
Human Glioblastoma

A fluorescent molecular probe for the identification of glioblastoma is developed. The probe allows the tracing of the cysteine (Cys) level, which is recognized as a new biomarker of GBM.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Subcellular Localization ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Fluorescent Sensor ◽  
Biological Functions ◽  
Localization Microscopy ◽  
Drug Activation ◽  
Nitroreductase Activity ◽  
Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Subcellular Localization ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Fluorescent Sensor ◽  
Biological Functions ◽  
Localization Microscopy ◽  
Drug Activation ◽  
Nitroreductase Activity ◽  
Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

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