An off-the-shelf microfluidic device for the controllable fabrication of multiple-holed hollow particles and their cell culture application

2021 ◽  
Author(s):  
Lili Sun ◽  
Tianbao Li ◽  
Baojin Zhang ◽  
Meng Zhang ◽  
Juan Xu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Size Distribution ◽  
Microfluidic Device ◽  
Hollow Particles ◽  
Multi Level ◽  
Controllable Fabrication ◽  
Droplet Method

The microfluidic droplet method has an excellent promise for preparing hollow particles because it can be versatile in realizing multi-level size distribution and scalable in production. However, the application of...

scholarly journals The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 229 ◽  
Author(s):  
Dongjin Lee ◽  
Chaenyung Cha
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Microfluidic Device ◽  
3D Cell Culture ◽  
Breast Adenocarcinoma ◽  
Adherent Cell ◽  
Flow Focusing ◽  
Spheroid Formation ◽  
3D Scaffold ◽  
Tumor Spheroids

Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photocrosslinked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.

NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

scholarly journals 512-Channel Geometric Droplet-Splitting Microfluidic Device by Injection of Premixed Emulsion for Microsphere Production

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 776
Author(s):  
Chul Min Kim ◽  
Hye Jin Choi ◽  
Gyu Man Kim
Keyword(s):  
Size Distribution ◽  
Microfluidic Device ◽  
Glycolic Acid ◽  
Liquid Droplet ◽  
Narrow Size Distribution ◽  
Water Droplets ◽  
Droplet Splitting ◽  
Conventional Photolithography ◽  
Post Array

We present a 512-channel geometric droplet-splitting microfluidic device that involves the injection of a premixed emulsion for microsphere production. The presented microfluidic device was fabricated using conventional photolithography and polydimethylsiloxane casting. The fabricated microfluidic device consisted of 512 channels with 256 T-junctions in the last branch. Five hundred and twelve microdroplets with a narrow size distribution were produced from a single liquid droplet. The diameter and size distribution of prepared micro water droplets were 35.29 µm and 8.8% at 10 mL/h, respectively. Moreover, we attempted to prepare biocompatible microspheres for demonstrating the presented approach. The diameter and size distribution of the prepared poly (lactic-co-glycolic acid) microspheres were 6.56 µm and 8.66% at 10 mL/h, respectively. To improve the monodispersity of the microspheres, we designed an additional post array part in the 512-channel geometric droplet-splitting microfluidic device. The monodispersity of the microdroplets prepared with the microfluidic device combined with the post array part exhibited a significant improvement.

A microfluidic device for immuno-affinity-based separation of mitochondria from cell culture

Lab on a Chip ◽  
2013 ◽  
Vol 13 (22) ◽  
pp. 4467 ◽  
Author(s):  
Sabrina Kayo ◽  
Janina Bahnemann ◽  
Matthias Klauser ◽  
Ralf Pörtner ◽  
An-Ping Zeng
Keyword(s):  
Cell Culture ◽  
Microfluidic Device

scholarly journals A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

Lab on a Chip ◽  
2010 ◽  
Vol 10 (14) ◽  
pp. 1807 ◽  
Author(s):  
Liyu Liu ◽  
Kevin Loutherback ◽  
David Liao ◽  
David Yeater ◽  
Guillaume Lambert ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Hydrodynamic Forces

A polydimethylsiloxane–polycarbonate hybrid microfluidic device capable of generating perpendicular chemical and oxygen gradients for cell culture studies

Lab on a Chip ◽  
2014 ◽  
Vol 14 (19) ◽  
pp. 3762-3772 ◽  
Author(s):  
Chia-Wen Chang ◽  
Yung-Ju Cheng ◽  
Melissa Tu ◽  
Ying-Hua Chen ◽  
Chien-Chung Peng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Culture Studies ◽  
Oxygen Gradients

This paper reports a PDMS–PC hybrid microfluidic device capable of performing cell culture under combinations of chemical and oxygen gradients.

Automated cell culture in high density tubeless microfluidic device arrays

Lab on a Chip ◽  
2008 ◽  
Vol 8 (5) ◽  
pp. 717 ◽  
Author(s):  
Ivar Meyvantsson ◽  
Jay W. Warrick ◽  
Steven Hayes ◽  
Allyson Skoien ◽  
David J. Beebe
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
High Density
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