A microfluidic device for immuno-affinity-based separation of mitochondria from cell culture

Lab on a Chip ◽  
2013 ◽  
Vol 13 (22) ◽  
pp. 4467 ◽  
Author(s):  
Sabrina Kayo ◽  
Janina Bahnemann ◽  
Matthias Klauser ◽  
Ralf Pörtner ◽  
An-Ping Zeng
Keyword(s):  
Cell Culture ◽  
Microfluidic Device

scholarly journals The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 229 ◽  
Author(s):  
Dongjin Lee ◽  
Chaenyung Cha
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Microfluidic Device ◽  
3D Cell Culture ◽  
Breast Adenocarcinoma ◽  
Adherent Cell ◽  
Flow Focusing ◽  
Spheroid Formation ◽  
3D Scaffold ◽  
Tumor Spheroids

Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photocrosslinked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.

NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

scholarly journals A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

Lab on a Chip ◽  
2010 ◽  
Vol 10 (14) ◽  
pp. 1807 ◽  
Author(s):  
Liyu Liu ◽  
Kevin Loutherback ◽  
David Liao ◽  
David Yeater ◽  
Guillaume Lambert ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Hydrodynamic Forces

A polydimethylsiloxane–polycarbonate hybrid microfluidic device capable of generating perpendicular chemical and oxygen gradients for cell culture studies

Lab on a Chip ◽  
2014 ◽  
Vol 14 (19) ◽  
pp. 3762-3772 ◽  
Author(s):  
Chia-Wen Chang ◽  
Yung-Ju Cheng ◽  
Melissa Tu ◽  
Ying-Hua Chen ◽  
Chien-Chung Peng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Culture Studies ◽  
Oxygen Gradients

This paper reports a PDMS–PC hybrid microfluidic device capable of performing cell culture under combinations of chemical and oxygen gradients.

Automated cell culture in high density tubeless microfluidic device arrays

Lab on a Chip ◽  
2008 ◽  
Vol 8 (5) ◽  
pp. 717 ◽  
Author(s):  
Ivar Meyvantsson ◽  
Jay W. Warrick ◽  
Steven Hayes ◽  
Allyson Skoien ◽  
David J. Beebe
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
High Density

scholarly journals Aspiration-mediated hydrogel micropatterning using rail-based open microfluidic devices for high-throughput 3D cell culture

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dohyun Park ◽  
Jungseub Lee ◽  
Younggyun Lee ◽  
Kyungmin Son ◽  
Jin Woo Choi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Capillary Pressure ◽  
Microfluidic Device ◽  
High Throughput ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
Cell Types ◽  
Human Organ ◽  
Patterning Technique ◽  
Experimental Yield

AbstractMicrofluidics offers promising methods for aligning cells in physiologically relevant configurations to recapitulate human organ functionality. Specifically, microstructures within microfluidic devices facilitate 3D cell culture by guiding hydrogel precursors containing cells. Conventional approaches utilize capillary forces of hydrogel precursors to guide fluid flow into desired areas of high wettability. These methods, however, require complicated fabrication processes and subtle loading protocols, thus limiting device throughput and experimental yield. Here, we present a swift and robust hydrogel patterning technique for 3D cell culture, where preloaded hydrogel solution in a microfluidic device is aspirated while only leaving a portion of the solution in desired channels. The device is designed such that differing critical capillary pressure conditions are established over the interfaces of the loaded hydrogel solution, which leads to controlled removal of the solution during aspiration. A proposed theoretical model of capillary pressure conditions provides physical insights to inform generalized design rules for device structures. We demonstrate formation of multiple, discontinuous hollow channels with a single aspiration. Then we test vasculogenic capacity of various cell types using a microfluidic device obtained by our technique to illustrate its capabilities as a viable micro-manufacturing scheme for high-throughput cellular co-culture.

scholarly journals 3D printed microfluidic devices for cell culture

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Marta Canta ◽  
Désirée Baruffaldi ◽  
Ignazio Roppolo ◽  
Annalisa Chiappone ◽  
Candido F. Pirri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Microfluidic Devices ◽  
3D Printed ◽  
Biomedical Field ◽  
Printed Materials

A successful application of the 3D printed materials in the biomedical field requires extensive studies to ensure their biocompatibility at every step of the process. Here, different components suitable for cell applications, including a microfluidic device, were 3D printed using common resins and a deep analysis of their biocompatibility and post printed protocols was conducted.

scholarly journals Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ana Carolina Urbaczek ◽  
Paulo Augusto Gomes Carneiro Leão ◽  
Fayene Zeferino Ribeiro de Souza ◽  
Ana Afonso ◽  
Juliana Vieira Alberice ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Microfluidic Device ◽  
Endothelial Cell Culture
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