Automated cell culture in high density tubeless microfluidic device arrays

Lab on a Chip ◽  
2008 ◽  
Vol 8 (5) ◽  
pp. 717 ◽  
Author(s):  
Ivar Meyvantsson ◽  
Jay W. Warrick ◽  
Steven Hayes ◽  
Allyson Skoien ◽  
David J. Beebe
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
High Density

Fabrication of high density metallic nanowires and nanotubes for cell culture studies

2011 ◽  
Vol 88 (8) ◽  
pp. 1702-1706 ◽  
Author(s):  
J.H. Tian ◽  
J. Hu ◽  
F. Zhang ◽  
X. Li ◽  
J. Shi ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Density ◽  
Metallic Nanowires ◽  
Culture Studies

scholarly journals The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 229 ◽  
Author(s):  
Dongjin Lee ◽  
Chaenyung Cha
Keyword(s):  
Mechanical Properties ◽  
Cell Culture ◽  
Microfluidic Device ◽  
3D Cell Culture ◽  
Breast Adenocarcinoma ◽  
Adherent Cell ◽  
Flow Focusing ◽  
Spheroid Formation ◽  
3D Scaffold ◽  
Tumor Spheroids

Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photocrosslinked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.

High density cell culture ofRhodotorula glutinis using oxygen-enriched air

1986 ◽  
Vol 8 (10) ◽  
pp. 715-718 ◽  
Author(s):  
Jae Gu Pan ◽  
Moo Young Kwak ◽  
Joon Shick Rhee
Keyword(s):  
Cell Culture ◽  
High Density ◽  
High Density Cell ◽  
High Density Cell Culture

NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

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