Polymerization-induced self-assembly for the fabrication of polymeric nano-objects with enhanced structural stability by cross-linking

2020 ◽  
Vol 11 (22) ◽  
pp. 3654-3672 ◽  
Author(s):  
Wen-Jian Zhang ◽  
Jamshid Kadirkhanov ◽  
Chang-Hui Wang ◽  
Sheng-Gang Ding ◽  
Chun-Yan Hong ◽  
...  
Keyword(s):  
Structural Stability ◽  
Self Assembly ◽  
Cross Linking ◽  
The Cross

This review discusses the strategies of core-cross-linking in most of the PISA literatures (including post-polymerization cross-linking, photo-cross-linking and in situ cross-linking) and the applications of the cross-linked nano-objects.

In Situ Cross-linking Polymerization-Induced Self-Assembly: Not Only Generates Cross-linked Structures But Also Promotes Morphology Transition by the Cross-linker

2021 ◽  
Author(s):  
Jamshid Kadirkhanov ◽  
Cheng-Lin Yang ◽  
Zi-Xuan Chang ◽  
Ren-Man Zhu ◽  
Cai-Yuan Pan ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Morphology Transition ◽  
Convenient Approach ◽  
Cross Linker ◽  
The Cross

Compared with the post-polymerization cross-linking strategy, in situ cross-linking by divinyl comonomers in polymerization-induced self-assembly (PISA) is a more straightforward and convenient approach to produce structurally stabilized nano-objects. However, cross-linking...

scholarly journals Conformational Transitions of the Cross-linking Domains of Elastin during Self-assembly

2014 ◽  
Vol 289 (14) ◽  
pp. 10057-10068 ◽  
Author(s):  
Sean E. Reichheld ◽  
Lisa D. Muiznieks ◽  
Richard Stahl ◽  
Karen Simonetti ◽  
Simon Sharpe ◽  
...  
Keyword(s):  
Self Assembly ◽  
Conformational Transitions ◽  
Cross Linking ◽  
The Cross

pH- and Reductant-Responsive Polymeric Vesicles with Robust Membrane-Cross-Linked Structures: In Situ Cross-Linking in Polymerization-Induced Self-Assembly

2019 ◽  
Vol 52 (3) ◽  
pp. 1140-1149 ◽  
Author(s):  
Miao Chen ◽  
Jia-Wei Li ◽  
Wen-Jian Zhang ◽  
Chun-Yan Hong ◽  
Cai-Yuan Pan
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Polymeric Vesicles

In situ and non-cytotoxic cross-linking strategy for 3D printable biomaterials

Soft Matter ◽  
2021 ◽  
Author(s):  
Yiğitcan Sümbelli ◽  
Sibel Emir Diltemiz ◽  
Mehmet Girayhan Say ◽  
Özlem Biçen Ünlüer ◽  
Arzu Ersöz ◽  
...  
Keyword(s):  
Elastic Properties ◽  
Structural Stability ◽  
Cross Linking

A new cross-linking method maintaining structural stability and elastic properties.

scholarly journals Dynamic cross-linking of an alginate–acrylamide tough hydrogel system: time-resolved in situ mapping of gel self-assembly

RSC Advances ◽  
2021 ◽  
Vol 11 (18) ◽  
pp. 10710-10726
Author(s):  
Akanksha Pragya ◽  
Suhas Mutalik ◽  
Muhammad Waseem Younas ◽  
Siu-Kwong Pang ◽  
Pui-Kin So ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Formation Process ◽  
Self Assembly ◽  
Cross Linking ◽  
Time Resolved ◽  
System Time ◽  
Dynamic Formation ◽  
Hydrogel System

In situ, time-resolved characterisation of an alginate–acrylamide tough hydrogel dynamic formation process indicate routes to intervention and modification of chemo-physico-mechanical properties.

Self-assembly of graphene oxide aerogels by layered double hydroxides cross-linking and their application in water purification

2014 ◽  
Vol 2 (23) ◽  
pp. 8941-8951 ◽  
Author(s):  
Qile Fang ◽  
Baoliang Chen
Keyword(s):  
Graphene Oxide ◽  
Structural Stability ◽  
Water Purification ◽  
Layered Double Hydroxides ◽  
Self Assembly ◽  
Adsorption Properties ◽  
The Self ◽  
Cross Linking ◽  
Double Hydroxides

3D macrostructural aerogels were synthesized based on the self-assembly of graphene oxide and LDHs, which exhibited excellent hydrophilicity, structural stability, and superior adsorption properties in water purification.

scholarly journals Targeted in situ cross-linking mass spectrometry and integrative modeling reveal the architectures of Nsp1, Nsp2, and Nucleocapsid proteins from SARS-CoV-2

2021 ◽  
Author(s):  
Moriya Slavin ◽  
Joanna Zamel ◽  
Keren Zohar ◽  
Siona Eliyahu ◽  
Merav Braitbard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Full Length ◽  
Integrative Model ◽  
Cross Linking ◽  
Cross Link ◽  
Integrative Modeling ◽  
Cellular Context ◽  
Cross Links ◽  
The Cross

AbstractAtomic structures of several proteins from the coronavirus family are still partial or unavailable. A possible reason for this gap is the instability of these proteins outside of the cellular context, thereby prompting the use of in-cell approaches. In situ cross-linking and mass spectrometry (in situ CLMS) can provide information on the structures of such proteins as they occur in the intact cell. Here, we applied targeted in situ CLMS to structurally probe Nsp1, Nsp2, and Nucleocapsid (N) proteins from SARS-CoV-2, and obtained cross-link sets with an average density of one cross-link per twenty residues. We then employed integrative modeling that computationally combined the cross-linking data with domain structures to determine full-length atomic models. For the Nsp2, the cross-links report on a complex topology with long-range interactions. Integrative modeling with structural prediction of individual domains by the AlphaFold2 system allowed us to generate a single consistent all-atom model of the full-length Nsp2. The model reveals three putative metal binding sites, and suggests a role for Nsp2 in zinc regulation within the replication-transcription complex. For the N protein, we identified multiple intra- and inter-domain cross-links. Our integrative model of the N dimer demonstrates that it can accommodate three single RNA strands simultaneously, both stereochemically and electrostatically. For the Nsp1, cross-links with the 40S ribosome were highly consistent with recent cryo-EM structures. These results highlight the importance of cellular context for the structural probing of recalcitrant proteins and demonstrate the effectiveness of targeted in situ CLMS and integrative modeling.

scholarly journals Bioinspired Photo-Crosslinkable Self-Assembling Peptide with pH-Switchable “On−Off” Luminescence.

2021 ◽  
Author(s):  
Raffaele Pugliese ◽  
Monica Montuori ◽  
Fabrizio Gelain
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Significant Progress ◽  
The Past ◽  
Self Assembling ◽  
Made In

Significant progress has been made in the peptides self-assembly over the past two decades, however the in situ cross-linking of self-assembling peptides yielding to better performing nanomaterials is still in...

scholarly journals Cranberry Juice Extract Rapidly Protects Demineralized Dentin against Digestion and Inhibits Its Gelatinolytic Activity

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3637
Author(s):  
Yong Wang ◽  
Austin Green ◽  
Xiaomei Yao ◽  
Hang Liu ◽  
Saleha Nisar ◽  
...  
Keyword(s):  
Grape Seed ◽  
Dental Composite ◽  
Cross Linking ◽  
Cranberry Juice ◽  
Composite Restorations ◽  
Dentin Collagen ◽  
Cross Linker ◽  
The Cross ◽  
Demineralized Dentin

Improving the longevity of composite restorations has proven to be difficult when they are bonded to dentin. Dentin demineralization leaves collagen fibrils susceptible to enzymatic digestion, which causes breakdown of the resin–dentin interface. Therefore, measures for counteracting the enzymatic environment by enhancing dentin collagen’s resistance to degradation have the potential to improve the durability of dental composite restorations. This study aimed to evaluate the effects of polyphenol-rich extracts and a chemical cross-linker on the cross-linking interaction, resistance to digestion, and endogenous matrix metalloproteinase (MMP) activities of dentin collagen under clinically relevant conditions. Ten-µm-thick films were cut from dentin slabs of non-carious extracted human third molars. Following demineralization, polyphenol-rich extracts—including grape seed (GSE), green tea (GTE), and cranberry juice (CJE)—or chemical cross-linker carbodiimide with n-hydroxysuccinimide (EDC/NHS) were applied to the demineralized dentin surfaces for 30 s. The collagen cross-linking, bio-stabilization, and gelatinolytic activities of MMPs 2 and 9 were studied by using Fourier-transform infrared spectroscopy, weight loss, hydroxyproline release, scanning/transmission electron microscopy, and in situ zymography. All treatments significantly increased resistance to collagenase degradation and reduced the gelatinolytic MMP activity of dentin collagen compared to the untreated control. The CJE- and GSE-treated groups were more resistant to digestion than the GTE- or EDC/NHS-treated ones (p < 0.05), which was consistent with the cross-linking interaction found with FTIR and the in situ performance on the acid-etched dentin surface found with SEM/TEM. The collagen films treated with CJE showed the lowest MMP activity, followed by GSE, GTE, and, finally, EDC/NHS. The CJE-treated dentin collagen rapidly increased its resistance to digestion and MMP inhibition. An application of CJE as short as 30 s may be a clinically feasible approach to improving the longevity of dentin bonding in composite restorations.

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