scholarly journals Rational design of small molecule fluorescent probes for biological applications

2020 ◽  
Vol 18 (30) ◽  
pp. 5747-5763 ◽  
Author(s):  
Joomyung V. Jun ◽  
David M. Chenoweth ◽  
E. James Petersson
Keyword(s):  
Energy Transfer ◽  
Small Molecule ◽  
Fluorescent Probes ◽  
Rational Design ◽  
Biological Systems ◽  
Biological Applications

Guidelines based on photophysical tuning, reactivity, isomerization, and energy transfer for rational design of synthetic fluorescent probes for biological systems.

scholarly journals Small-molecule fluorescent probes for imaging gaseous signaling molecules: current progress and future implications

2020 ◽  
Vol 11 (20) ◽  
pp. 5127-5141 ◽  
Author(s):  
Mingwang Yang ◽  
Jiangli Fan ◽  
Jianjun Du ◽  
Xiaojun Peng
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Biological Systems ◽  
Design Principles ◽  
Signaling Molecules ◽  
Gaseous Signaling Molecules

This perspective article aims to introduce the design principles and recognition strategies of small-molecule fluorescent probes which are applied for the detection of gas signaling molecules including NO, CO and H2S in biological systems.

Small-Molecule Fluorescent Probes for Imaging and Detection of Reactive Oxygen, Nitrogen, and Sulfur Species in Biological Systems

2017 ◽  
Vol 90 (1) ◽  
pp. 533-555 ◽  
Author(s):  
Xiaoyun Jiao ◽  
Yong Li ◽  
Jinye Niu ◽  
Xilei Xie ◽  
Xu Wang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Biological Systems ◽  
Reactive Oxygen ◽  
Sulfur Species

Progress of Small Molecule Fluorescent Probes for Detection of Thiols

2014 ◽  
Vol 1052 ◽  
pp. 464-467
Author(s):  
Yan Gao
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Fluorescent Probes ◽  
Clinical Chemistry ◽  
Biological Systems ◽  
Quantitative Detection ◽  
Antioxidant Systems ◽  
Nondestructive Imaging ◽  
Sensitivity Specificity

Thiols play key roles in biological systems. They are important parts of many organisms proteins and small molecules, and have an important role in cellular antioxidant systems. In recent years, fluorescent methods for detecting the mercapto compounds have developed rapidly, based on its apparent advantages in sensitivity, specificity and nondestructive imaging. Therefore, the quantitative detection of mercapto biomolecules are very important in biochemical and clinical chemistry. In this review,we summarized the fluorescent probes for thiols according to their unique mechanisms between fluorescent probes and thiols.

FRET-Based Small-Molecule Fluorescent Probes: Rational Design and Bioimaging Applications

2013 ◽  
Vol 46 (7) ◽  
pp. 1462-1473 ◽  
Author(s):  
Lin Yuan ◽  
Weiying Lin ◽  
Kaibo Zheng ◽  
Sasa Zhu
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Rational Design

scholarly journals Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents

2020 ◽  
Vol 49 (15) ◽  
pp. 5110-5139 ◽  
Author(s):  
Luling Wu ◽  
Chusen Huang ◽  
Ben P. Emery ◽  
Adam C. Sedgwick ◽  
Steven D. Bull ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Small Molecule ◽  
Fluorescent Probes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Imaging Agents ◽  
Recent Advances ◽  
Förster Resonance

In this tutorial review, we will explore recent advances in the construction and application of Förster resonance energy transfer (FRET)-based small-molecule fluorescent probes.

Rational design of near-infrared fluorophores with phenolic D−A type structure and construction of a fluorescent probe for cysteine imaging

2021 ◽  
Author(s):  
Dugang Chen ◽  
Gang Nie ◽  
Yecheng Dang ◽  
Wenjie Liang ◽  
Wanqing Li ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Rational Design ◽  
Near Infrared ◽  
Type Structure ◽  
Deep Penetration ◽  
Biological Applications ◽  
Low Background ◽  
Nir Emission

Fluorescent probes with near-infrared (NIR) emission have attracted great attentions in the biological applications because of the low background interference and deep penetration depth. Therefore, as an important component of...

A Small Molecule – Protein Hybrid for Voltage Imaging via Quenching of Bioluminescence

2020 ◽  
Author(s):  
Brittany Benlian ◽  
Pavel Klier ◽  
Kayli Martinez ◽  
Marie Schwinn ◽  
Thomas Kirkland ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Membrane Potential ◽  
Small Molecule ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Potential Oscillations ◽  
Excitation Light ◽  
Voltage Imaging ◽  
Induced Pluripotent

<p>We report a small molecule enzyme pair for optical voltage sensing via quenching of bioluminescence. This <u>Q</u>uenching <u>B</u>ioluminescent V<u>olt</u>age Indicator, or Q-BOLT, pairs the dark absorbing, voltage-sensitive dipicrylamine with membrane-localized bioluminescence from the luciferase NanoLuc (NLuc). As a result, bioluminescence is quenched through resonance energy transfer (QRET) as a function of membrane potential. Fusion of HaloTag to NLuc creates a two-acceptor bioluminescence resonance energy transfer (BRET) system when a tetramethylrhodamine (TMR) HaloTag ligand is ligated to HaloTag. In this mode, Q-BOLT is capable of providing direct visualization of changes in membrane potential in live cells via three distinct readouts: change in QRET, BRET, and the ratio between bioluminescence emission and BRET. Q-BOLT can provide up to a 29% change in bioluminescence (ΔBL/BL) and >100% ΔBRET/BRET per 100 mV change in HEK 293T cells, without the need for excitation light. In cardiac monolayers derived from human induced pluripotent stem cells (hiPSC), Q-BOLT readily reports on membrane potential oscillations. Q-BOLT is the first example of a hybrid small molecule – protein voltage indicator that does not require excitation light and may be useful in contexts where excitation light is limiting.</p> <p> </p>

A Small Molecule – Protein Hybrid for Voltage Imaging via Quenching of Bioluminescence

2020 ◽  
Author(s):  
Brittany Benlian ◽  
Pavel Klier ◽  
Kayli Martinez ◽  
Marie Schwinn ◽  
Thomas Kirkland ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Membrane Potential ◽  
Small Molecule ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Potential Oscillations ◽  
Excitation Light ◽  
Voltage Imaging ◽  
Induced Pluripotent

<p>We report a small molecule enzyme pair for optical voltage sensing via quenching of bioluminescence. This <u>Q</u>uenching <u>B</u>ioluminescent V<u>olt</u>age Indicator, or Q-BOLT, pairs the dark absorbing, voltage-sensitive dipicrylamine with membrane-localized bioluminescence from the luciferase NanoLuc (NLuc). As a result, bioluminescence is quenched through resonance energy transfer (QRET) as a function of membrane potential. Fusion of HaloTag to NLuc creates a two-acceptor bioluminescence resonance energy transfer (BRET) system when a tetramethylrhodamine (TMR) HaloTag ligand is ligated to HaloTag. In this mode, Q-BOLT is capable of providing direct visualization of changes in membrane potential in live cells via three distinct readouts: change in QRET, BRET, and the ratio between bioluminescence emission and BRET. Q-BOLT can provide up to a 29% change in bioluminescence (ΔBL/BL) and >100% ΔBRET/BRET per 100 mV change in HEK 293T cells, without the need for excitation light. In cardiac monolayers derived from human induced pluripotent stem cells (hiPSC), Q-BOLT readily reports on membrane potential oscillations. Q-BOLT is the first example of a hybrid small molecule – protein voltage indicator that does not require excitation light and may be useful in contexts where excitation light is limiting.</p> <p> </p>

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