Polyelectrolyte–calcium complexes as a pre-precursor induce biomimetic mineralization of collagen

Nanoscale ◽  
2021 ◽  
Author(s):  
Zihuai Zhou ◽  
Leiqing Zhang ◽  
Jiachen Li ◽  
Ying Shi ◽  
Zhifang Wu ◽  
...  
Keyword(s):  
Biomimetic Mineralization ◽  
Calcium Complexes

Rapid biomimetic intrafibrillar mineralization of collagen was induced by polyelectrolyte–calcium complexes as a pre-precursor.

scholarly journals Morphogenesis and evolution mechanisms of bacterially-induced struvite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tian-Lei Zhao ◽  
Han Li ◽  
Hao-Fan Jiang ◽  
Qi-Zhi Yao ◽  
Ying Huang ◽  
...  
Keyword(s):  
Time Course ◽  
Carboxyl Groups ◽  
Precipitation Process ◽  
Phosphate Content ◽  
Biomimetic Mineralization ◽  
Struvite Precipitation ◽  
Growth Habits ◽  
Two Stages ◽  
Relevant Work ◽  
Insight Into

AbstractBacteria are able to induce struvite precipitation, and modify struvite morphology, leading to the mineral with various growth habits. However, the relevant work involving the morphogenesis is limited, thereby obstructing our understanding of bacterially mediated struvite mineralization. Here, an actinomycete Microbacterium marinum sp. nov. H207 was chosen to study its effect on struvite morphology. A combination of bacterial mineralization and biomimetic mineralization techniques was adopted. The bacterial mineralization results showed that strain H207 could induce the formation of struvite with grouping structure (i.e., a small coffin-like crystal grown on a large trapezoid-like substrate crystal), and the overgrowth structure gradually disappeared, while the substrate crystal further evolved into coffin-like, and quadrangular tabular morphology with time. The biomimetic experiments with different organic components confirmed that the soluble macromolecules rich in electronegative carboxyl groups secreted by strain H207 dominate the formation of the struvite grouping. The time-course biomimetic experiments with supernatant testified that the increase in pH and NH4+ content promoted the evolution of crystal habits. Moreover, the evolution process of substrate crystal can be divided into two stages. At the first stage, the crystal grew along the crystallographic b axis. At the later stage, coupled dissolution–precipitation process occurred, and the crystals grew along the corners (i.e., [110] and [1-10] directions). In the case of dissolution, it was also found that the (00-1) face of substrate crystal preferentially dissolved, which results from the low initial phosphate content and high PO43− density on this face. As a result, present work can provide a deeper insight into bio-struvite mineralization.

Changes in the secondary structures and zeta potential of soybean peptide and its calcium complexes in cell membrane medium

2021 ◽  
Author(s):  
He Liu ◽  
Ying Lv ◽  
Jingting Xu ◽  
Chen Chen ◽  
Shuntang Guo
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cell Membrane ◽  
Zeta Potential ◽  
Calcium Binding ◽  
Binding Capacity ◽  
Secondary Structures ◽  
High Calcium ◽  
Calcium Complexes

In this study, soybean peptides (10-30kDa) with high calcium binding capacity were prepared by enzymatic hydrolysis and ultrafiltration. The results of cell experiments showed that the peptide could transport calcium...

C–H and C–F coordination of arenes in neutral alkaline earth metal complexes

2021 ◽  
Author(s):  
Jacob S. McMullen ◽  
Alison J. Edwards ◽  
Jamie Hicks
Keyword(s):  
Metal Complexes ◽  
Alkaline Earth ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Calcium Complexes ◽  
Group 2 ◽  
Rare Group

A series of neutral magnesium and calcium complexes bearing an extremely bulky diamido ligand have been synthesised and crystallographically characterised. A number of these complexes feaure rare group 2 metal···aromatic...

scholarly journals Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 992
Author(s):  
Song Chen ◽  
Yuanli He ◽  
Linna Zhong ◽  
Wenjia Xie ◽  
Yiyuan Xue ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Photoelectron Spectroscopy ◽  
Antibacterial Properties ◽  
Biomimetic Mineralization ◽  
Matrix Mineralization ◽  
X Ray ◽  
Porous Ti ◽  
Potential Applications ◽  
Modification Of Titanium

The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against S. sanguis and S. aureus were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of S. sanguis and S. aureus and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.

scholarly journals Molecular Properties of Bare and Microhydrated Vitamin B5–Calcium Complexes

2021 ◽  
Vol 22 (2) ◽  
pp. 692
Author(s):  
Davide Corinti ◽  
Barbara Chiavarino ◽  
Debora Scuderi ◽  
Caterina Fraschetti ◽  
Antonello Filippi ◽  
...  
Keyword(s):  
Gas Phase ◽  
Pantothenic Acid ◽  
Isomeric Form ◽  
Deprotonated Form ◽  
Linear Absorption ◽  
Vitamin B5 ◽  
Essential Nutrient ◽  
Calcium Complexes ◽  
Singly Charged ◽  
Phase Population

Pantothenic acid, also called vitamin B5, is an essential nutrient involved in several metabolic pathways. It shows a characteristic preference for interacting with Ca(II) ions, which are abundant in the extracellular media and act as secondary mediators in the activation of numerous biological functions. The bare deprotonated form of pantothenic acid, [panto-H]−, its complex with Ca(II) ion, [Ca(panto-H)]+, and singly charged micro-hydrated calcium pantothenate [Ca(panto-H)(H2O)]+ adduct have been obtained in the gas phase by electrospray ionization and assayed by mass spectrometry and IR multiple photon dissociation spectroscopy in the fingerprint spectral range. Quantum chemical calculations at the B3LYP(-D3) and MP2 levels of theory were performed to simulate geometries, thermochemical data, and linear absorption spectra of low-lying isomers, allowing us to assign the experimental absorptions to particular structural motifs. Pantothenate was found to exist in the gas phase as a single isomeric form showing deprotonation on the carboxylic moiety. On the contrary, free and monohydrated calcium complexes of deprotonated pantothenic acid both present at least two isomers participating in the gas-phase population, sharing the deprotonation of pantothenate on the carboxylic group and either a fourfold or fivefold coordination with calcium, thus justifying the strong affinity of pantothenate for the metal.

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