Three-dimensional rope-like and cloud-like nanofibrous scaffolds facilitating in-depth cell infiltration developed using a highly conductive electrospinning system

Nanoscale ◽  
2020 ◽  
Vol 12 (32) ◽  
pp. 16690-16696
Author(s):  
Ran Li ◽  
Xiong Deng ◽  
Fei Liu ◽  
Yuan Yang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Infiltration ◽  
Electrospinning Technique ◽  
Nanofibrous Scaffolds ◽  
System P

A highly conductive 3D electrospinning technique capable of constructing rope-like or cloud-like nanofibrous scaffolds is established for in-depth cell infiltration and the mechanism is revealed.

Improved cell infiltration and vascularization of three-dimensional bacterial cellulose nanofibrous scaffolds by template biosynthesis

RSC Advances ◽  
2016 ◽  
Vol 6 (48) ◽  
pp. 42229-42239 ◽  
Author(s):  
Zhe Li ◽  
Xiangguo Lv ◽  
Shiyan Chen ◽  
Baoxiu Wang ◽  
Chao Feng ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Tissue Regeneration ◽  
3D Structure ◽  
Three Dimensional ◽  
Significant Problem ◽  
Cell Infiltration ◽  
Nanofibrous Scaffolds

A significant problem limiting the application of bacterial cellulose (BC) nanofibrous scaffolds for tissue regeneration is the nanoscale pores that inhibit cell infiltration and vascularization in their three-dimensional (3D) structure.

scholarly journals Poly(Vinyl Alcohol)-Based Nanofibrous Electrospun Scaffolds for Tissue Engineering Applications

Polymers ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 7 ◽  
Author(s):  
Marta A. Teixeira ◽  
M. Teresa P. Amorim ◽  
Helena P. Felgueiras
Keyword(s):  
Tissue Engineering ◽  
Vinyl Alcohol ◽  
Mechanical Performance ◽  
Three Dimensional ◽  
Poly Vinyl Alcohol ◽  
Electrospinning Technique ◽  
Electrospun Scaffolds ◽  
Nanofibrous Scaffolds ◽  
The Stability ◽  
The Many

Tissue engineering (TE) holds an enormous potential to develop functional scaffolds resembling the structural organization of native tissues, to improve or replace biological functions and prevent organ transplantation. Amongst the many scaffolding techniques, electrospinning has gained widespread interest because of its outstanding features that enable the production of non-woven fibrous structures with a dimensional organization similar to the extracellular matrix. Various polymers can be electrospun in the form of three-dimensional scaffolds. However, very few are successfully processed using environmentally friendly solvents; poly(vinyl alcohol) (PVA) is one of those. PVA has been investigated for TE scaffolding production due to its excellent biocompatibility, biodegradability, chemo-thermal stability, mechanical performance and, most importantly, because of its ability to be dissolved in aqueous solutions. Here, a complete overview of the applications and recent advances in PVA-based electrospun nanofibrous scaffolds fabrication is provided. The most important achievements in bone, cartilage, skin, vascular, neural and corneal biomedicine, using PVA as a base substrate, are highlighted. Additionally, general concepts concerning the electrospinning technique, the stability of PVA when processed, and crosslinking alternatives to glutaraldehyde are as well reviewed.

scholarly journals The Symmetric 3D Organization of Connective Tissue around Implant Abutment: A Key-Issue to Prevent Bone Resorption

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Connective Tissue ◽  
Inflammatory Cell ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Collagen Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Oral Epithelium ◽  
Implant Abutment

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.

scholarly journals Conformal Fabrication of an Electrospun Nanofiber Mat on a 3D Ear Cartilage-Shaped Hydrogel Collector Based on Hydrogel-Assisted Electrospinning

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Jin Yeong Song ◽  
Hyun Il Ryu ◽  
Jeong Myeong Lee ◽  
Seong Hwan Bae ◽  
Jae Woo Lee ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Cell Delivery ◽  
Complex Geometries ◽  
Two Dimensional ◽  
Uniform Thickness ◽  
Electrospun Nanofiber ◽  
Electrospinning Technique ◽  
Ear Cartilage ◽  
Focused Electric Field

AbstractElectrospinning is a common and versatile process to produce nanofibers and deposit them on a collector as a two-dimensional nanofiber mat or a three-dimensional (3D) macroscopic arrangement. However, 3D electroconductive collectors with complex geometries, including protruded, curved, and recessed regions, generally caused hampering of a conformal deposition and incomplete covering of electrospun nanofibers. In this study, we suggested a conformal fabrication of an electrospun nanofiber mat on a 3D ear cartilage-shaped hydrogel collector based on hydrogel-assisted electrospinning. To relieve the influence of the complex geometries, we flattened the protruded parts of the 3D ear cartilage-shaped hydrogel collector by exploiting the flexibility of the hydrogel. We found that the suggested fabrication technique could significantly decrease an unevenly focused electric field, caused by the complex geometries of the 3D collector, by alleviating the standard deviation by more than 70% through numerical simulation. Furthermore, it was experimentally confirmed that an electrospun nanofiber mat conformally covered the flattened hydrogel collector with a uniform thickness, which was not achieved with the original hydrogel collector. Given that this study established the conformal electrospinning technique on 3D electroconductive collectors, it will contribute to various studies related to electrospinning, including tissue engineering, drug/cell delivery, environmental filter, and clothing.

scholarly journals Current Update of Collagen Nanomaterials—Fabrication, Characterisation and Its Applications: A Review

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 316
Author(s):  
Samantha Lo ◽  
Mh Busra Fauzi
Keyword(s):  
Mechanical Stability ◽  
Health Management ◽  
Three Dimensional ◽  
Volume Ratio ◽  
Cartilage Regeneration ◽  
Nerve Tissue ◽  
Electrospinning Technique ◽  
Engineering Technology ◽  
Promising Alternative ◽  
Tissue Repair And Regeneration

Tissue engineering technology is a promising alternative approach for improvement in health management. Biomaterials play a major role, acting as a provisional bioscaffold for tissue repair and regeneration. Collagen a widely studied natural component largely present in the extracellular matrix (ECM) of the human body. It provides mechanical stability with suitable elasticity and strength to various tissues, including skin, bone, tendon, cornea and others. Even though exogenous collagen is commonly used in bioscaffolds, largely in the medical and pharmaceutical fields, nano collagen is a relatively new material involved in nanotechnology with a plethora of unexplored potential. Nano collagen is a form of collagen reduced to a nanoparticulate size, which has its advantages over the common three-dimensional (3D) collagen design, primarily due to its nano-size contributing to a higher surface area-to-volume ratio, aiding in withstanding large loads with minimal tension. It can be produced through different approaches including the electrospinning technique to produce nano collagen fibres resembling natural ECM. Nano collagen can be applied in various medical fields involving bioscaffold insertion or fillers for wound healing improvement; skin, bone, vascular grafting, nerve tissue and articular cartilage regeneration as well as aiding in drug delivery and incorporation for cosmetic purposes.

Bioresorbable electrospun nanofibrous scaffolds loaded with bioactive molecules

e-Polymers ◽  
2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Chiara Gualandi ◽  
Piotr Wilczek ◽  
Maria Letizia Focarete ◽  
Gianandrea Pasquinelli ◽  
Michal Kawalec ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Degradation Kinetics ◽  
Hydrolytic Degradation ◽  
Mesenchymal Cells ◽  
Bioactive Molecules ◽  
Differential Interference Contrast Microscopy ◽  
Electrospinning Technique ◽  
Ki 67 ◽  
Nanofibrous Scaffolds

AbstractElectrospinning technology is used to fabricate sub-micrometric fiber mats made of a random equimolar poly(lactide-co-glycolide) copolymer (PLGA), whose in vitro hydrolytic degradation kinetics is investigated over a period of 49 days in phosphate buffer at 37 °C. The PLGA mats show a decrease of molecular weight (by GPC) from the very beginning of the experiment, whereas a macroscopic weight loss from the samples is appreciated (by gravimetry) only after 20 days of buffer exposure. The molar mass distribution curves remain monomodal during the degradation experiment suggesting that no acid auto-catalyzed hydrolysis, commonly observed in bulk specimens, occurs in sub-micrometric PLGA fibers. PLGA scaffolds containing Endothelial Growth Factor Supplement (ECGS) were also fabricated by electrospinning, from ECGS-containing polymer solutions. Mesenchymal cells derived from human bone marrow mononuclear cells were cultured in the presence of such ECGS-loaded PLGA scaffolds. Flow cytometry and Differential Interference Contrast microscopy were used to characterize the cell cultures over a 7 day period. The results of AnexinV/PI staining and of intranuclear Ki-67 protein expression show, together with concomitant cell morphology modifications, that growth factors released from the scaffolds support the survival, proliferation and growth of the mesenchymal cells. This result demonstrates that ECGS maintains its bioactivity upon release from the electrospun fibers and shows the versatility of the electrospinning technique.

Single-component molecular conductor [Pt(dmdt)2]—a three-dimensional ambient-pressure molecular Dirac electron system

2019 ◽  
Vol 55 (23) ◽  
pp. 3327-3330 ◽  
Author(s):  
Biao Zhou ◽  
Shoji Ishibashi ◽  
Tatsuru Ishii ◽  
Takahiko Sekine ◽  
Ryosuke Takehara ◽  
...  
Keyword(s):  
Ambient Pressure ◽  
Three Dimensional ◽  
Electron System ◽  
Single Component ◽  
Nodal Lines ◽  
Molecular Conductor ◽  
System P ◽  
Dirac Electron

[Pt(dmdt)2], an air-stable single-component molecular conductor, contains massless Dirac electrons and carries Dirac nodal lines at ambient pressure.

Aqueous synthesis of three-dimensional fluorescent silicon-based nanoscale networks featuring unusual anti-photobleaching properties

2019 ◽  
Vol 55 (5) ◽  
pp. 652-655 ◽  
Author(s):  
Yiling Zhong ◽  
Binbin Chu ◽  
Xin Bo ◽  
Yao He ◽  
Chuan Zhao
Keyword(s):  
Electronic Structure ◽  
Three Dimensional ◽  
Aqueous Synthesis ◽  
Structure System ◽  
System P ◽  
Silicon Based

Three-dimensional fluorescent silicon-based nanoscale networks (SiNNs) possess unusual anti-photobleaching properties, owing to a unique electronic structure system.

scholarly journals Antibacterial, Flexible, and Conductive Membrane Based on MWCNTs/Ag Coated Electro-Spun PLA Nanofibrous Scaffolds as Wearable Fabric for Body Motion Sensing

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 120 ◽  
Author(s):  
Lu Gan ◽  
Aobo Geng ◽  
Ying Wu ◽  
Linjie Wang ◽  
Xingyu Fang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Silver Nanoparticles ◽  
Three Dimensional ◽  
Antibacterial Properties ◽  
Human Motion ◽  
Body Motion ◽  
Body Parts ◽  
Motion Sensing ◽  
Nanofibrous Scaffolds ◽  
Conductive Membrane

In the present study, flexible and conductive nanofiber membranes were prepared by coating PLA nanofibrous scaffolds with carbon nanotubes and silver nanoparticles. The morphology and structure of the prepared membrane was characterized, as well as its mechanical properties, electrical sensing behavior during consecutive stretching-releasing cycles and human motion detecting performance. Furthermore, the antibacterial properties of the membrane was also investigated. Due to the synergistic and interconnected three-dimensional (3D) conductive networks, formed by carbon nanotubes and silver nanoparticles, the membrane exhibited repeatable and durable strain-dependent sensitivity. Further, the prepared membrane could accurately detect the motions of different body parts. Accompanied with promising antibacterial properties and washing fastness, the prepared flexible and conductive membrane provides great application potential as a wearable fabric for real-time body motion sensing.

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