Rapid and precise tumor cell separation using the combination of size-dependent inertial and size-independent magnetic methods

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Di Huang ◽  
Nan Xiang
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Tumor Antigen ◽  
Cell Separation ◽  
Stage I ◽  
Rare Tumor ◽  
Inertial Microfluidics ◽  
Size Dependent ◽  
Magnetic Methods

A three-stage i-Mag device combines the passive inertial microfluidics and the active magnetophoresis method for rapid, precise, and tumor antigen-independent separation of rare tumor cells from blood.

Inertial Microfluidics for Separation and Detection of Tumor Cells

2013 ◽  
Author(s):  
Jiashu Sun
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Separation ◽  
Microfluidic Channel ◽  
Isothermal Amplification ◽  
Rare Tumor ◽  
Label Free ◽  
Ck19 Mrna ◽  
Hydrodynamic Effects ◽  
Mcf 7

We report on the development of a curved microfluidic channel that allows rapid and continuous size-based rare tumor cell separation from blood in a label-free manner by exploiting the hydrodynamic effects. The separated tumor cells are trapped and enriched on an integrated polycarbonate filter glued on top of the outlet reservoir of microchannels. CK19 mRNA of MCF-7 cells are detected by loop-mediated isothermal amplification (LAMP).

scholarly journals Detection of Circulating Tumor Cells (CTCs) in Malignant Pleural Mesothelioma (MPM) with the “Universal” CTC-Chip and An Anti-Podoplanin Antibody NZ-1.2

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 888
Author(s):  
Taiji Kuwata ◽  
Kazue Yoneda ◽  
Masataka Mori ◽  
Masatoshi Kanayama ◽  
Koji Kuroda ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Malignant Pleural Mesothelioma ◽  
Circulating Tumor Cell ◽  
Microfluidic System ◽  
Clinical Implications ◽  
Pleural Mesothelioma ◽  
Rare Tumor ◽  
Podoplanin Expression ◽  
Higher Sensitivity

Circulating tumor cell (CTC) is a potentially useful surrogate of micro-metastasis, but detection of rare tumor cells contaminated in a vast majority of normal hematologic cells remains technical challenges. To achieve effective detection of a variety of CTCs, we have developed a novel microfluidic system (CTC-chip) in which any antibody to capture CTCs is easily conjugated. In previous studies, we employed an antibody (clone E-1) against podoplanin that was strongly expressed on mesothelioma cells. The CTC-chip coated by the E-1 antibody (E1-chip) provided a modest sensitivity in detection of CTCs in malignant pleural mesothelioma (MPM). Here, to achieve a higher sensitivity, we employed a novel anti-podoplanin antibody (clone NZ-1.2). In an experimental model, MPM cells with high podoplanin expression were effectively captured with the CTC-chip coated by the NZ-1.2 antibody (NZ1.2-chip). Next, we evaluated CTCs in the peripheral blood sampled from 22 MPM patients using the NZ1.2-chip and the E1-chip. One or more CTCs were detected in 15 patients (68.2%) with the NZ1.2-chip, whereas only in 10 patients (45.5%) with the E1-chip. Of noted, in most (92.3%, 12/13) patients with epithelioid MPM subtype, CTCs were positive with the NZ1.2-chip. The CTC-count detected with the NZ1.2-chip was significantly higher than that with the E1-chip (p = 0.034). The clinical implications of CTCs detected with the NZ1.2-chip will be examined in a future study.

Selective, User-friendly, Highly Porous, Efficient, and Rapid (SUPER) Filter for Isolation and Analysis of Rare Tumor Cells

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Kaifeng Zhao ◽  
Yaoping Liu ◽  
Hua Wang ◽  
Yanling Song ◽  
Xiao-Feng Chen ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Clinical Application ◽  
Liquid Biopsy ◽  
Body Fluids ◽  
Selective Separation ◽  
Rare Tumor ◽  
Filtration System ◽  
User Friendly ◽  
Highly Porous

Rapid, efficient, and selective separation of tumor cells from complex body fluids is urgently needed for clinical application of tumor-cell-based liquid biopsy. Herein, a size-selective affinity filtration system, named Selective,...

Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3436-3445 ◽  
Author(s):  
Fei Tian ◽  
Lili Cai ◽  
Jianqiao Chang ◽  
Shanshan Li ◽  
Chao Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Whole Blood ◽  
Rare Tumor ◽  
Label Free ◽  
Size Dependent

Label-free, size-dependent, and high-throughput isolation of rare tumor cells from untreated whole blood is enabled by interfacial viscoelastic microfluidics.

scholarly journals Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 1860-1876 ◽  
Author(s):  
Wujun Zhao ◽  
Yang Liu ◽  
Brittany D. Jenkins ◽  
Rui Cheng ◽  
Bryana N. Harris ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Cell Size ◽  
Circulating Tumor Cells ◽  
Tumor Antigen ◽  
Cell Separation ◽  
Size Variation ◽  
Cell Size Variation

Integrated ferrohydrodynamic cell separation (iFCS) explores cell magnetization in biocompatible ferrofluids and enriches CTCs in an antigen-independent and cell size variation-inclusive manner.

A high-throughput liquid biopsy for rapid rare cell separation from large-volume samples

Lab on a Chip ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 68-78 ◽  
Author(s):  
Yaoping Liu ◽  
Tingyu Li ◽  
Mingxin Xu ◽  
Wei Zhang ◽  
Yan Xiong ◽  
...  
Keyword(s):  
Tumor Cell ◽  
High Throughput ◽  
Cancer Detection ◽  
Large Volume ◽  
Liquid Biopsy ◽  
Cell Separation ◽  
Body Fluids ◽  
Rare Tumor

The developed high-throughput liquid biopsy platform for rare tumor cell separation from body fluids has shown enormous promise in cancer detection and prognosis monitoring.

Fundamentals of integrated ferrohydrodynamic cell separation in circulating tumor cell isolation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Bryana N. Harris ◽  
Jonathan R. Murrow ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Recovery Rate ◽  
Cell Separation ◽  
Circulating Tumor Cell ◽  
Fundamental Theory ◽  
High Recovery ◽  
High Recovery Rate ◽  
Experimental Validations

We present the fundamental theory and experimental validations of an integrated ferrohydrodynamic cell separation (iFCS) method that can isolate circulating tumor cells with a high recovery rate.

scholarly journals Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels

2013 ◽  
Vol 7 (1) ◽  
pp. 011802 ◽  
Author(s):  
Jiashu Sun ◽  
Chao Liu ◽  
Mengmeng Li ◽  
Jidong Wang ◽  
Yunlei Xianyu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Separation ◽  
Microfluidic Channels ◽  
Rare Tumor
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