Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics

Lab on a Chip ◽  
2018 ◽  
Vol 18 (22) ◽  
pp. 3436-3445 ◽  
Author(s):  
Fei Tian ◽  
Lili Cai ◽  
Jianqiao Chang ◽  
Shanshan Li ◽  
Chao Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Whole Blood ◽  
Rare Tumor ◽  
Label Free ◽  
Size Dependent

Label-free, size-dependent, and high-throughput isolation of rare tumor cells from untreated whole blood is enabled by interfacial viscoelastic microfluidics.

scholarly journals Label-free high-throughput photoacoustic tomography of suspected circulating melanoma tumor cells in patients in vivo

2020 ◽  
Vol 25 (03) ◽  
pp. 1 ◽  
Author(s):  
Pengfei Hai ◽  
Yuan Qu ◽  
Yang Li ◽  
Liren Zhu ◽  
Leonid Shmuylovich ◽  
...  
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Photoacoustic Tomography ◽  
Label Free ◽  
Melanoma Tumor

scholarly journals High-Throughput Microfluidic Labyrinth for the Label-free Isolation of Circulating Tumor Cells

Cell Systems ◽  
2017 ◽  
Vol 5 (3) ◽  
pp. 295-304.e4 ◽  
Author(s):  
Eric Lin ◽  
Lianette Rivera-Báez ◽  
Shamileh Fouladdel ◽  
Hyeun Joong Yoon ◽  
Stephanie Guthrie ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Label Free

scholarly journals 1204 Label-free high-throughput photoacoustic imaging of circulating melanoma tumor cells in patients in vivo

2018 ◽  
Vol 138 (5) ◽  
pp. S204
Author(s):  
P. Hai ◽  
L. Shmuylovich ◽  
Y. Qu ◽  
L.A. Cornelius ◽  
L. Wang
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Photoacoustic Imaging ◽  
Label Free ◽  
Melanoma Tumor

scholarly journals Dynamic Halbach array magnet integrated microfluidic system for the continuous-flow separation of rare tumor cells

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38496-38504 ◽  
Author(s):  
Mei Xue ◽  
An Xiang ◽  
Yanhai Guo ◽  
Li Wang ◽  
Rou Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Flow Separation ◽  
Whole Blood ◽  
Continuous Flow ◽  
Microfluidic System ◽  
Rare Tumor ◽  
Halbach Array

We develop a dynamic Halbach array magnet integrated microfluidic system for continuous-flow separation of circulating tumor cells from whole blood.

Label-free, high-throughput holographic screening and enumeration of tumor cells in blood

Lab on a Chip ◽  
2017 ◽  
Vol 17 (17) ◽  
pp. 2920-2932 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Cell Size ◽  
High Throughput ◽  
Digital Holography ◽  
Label Free

We developed a microfluidic inline digital holography microscopy platform to fingerprint tumor cells in blood based on cell size and optical signatures.

scholarly journals Ultrafast and Predictive Mass Spectrometry–Based Autotaxin Assays for Label-Free Potency Screening

2017 ◽  
Vol 22 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Tom Bretschneider ◽  
Andreas Harald Luippold ◽  
Helmut Romig ◽  
Daniel Bischoff ◽  
Klaus Klinder ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Whole Blood ◽  
Label Free ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Data Points ◽  
Set Up

Autotaxin (ATX) is a promising drug target for the treatment of several diseases, such as cancer and fibrosis. ATX hydrolyzes lysophosphatidyl choline (LPC) into bioactive lysophosphatidic acid (LPA). The potency of ATX inhibitors can be readily determined by using fluorescence-based LPC derivatives. While such assays are ultra-high throughput, they are prone to false positives compared to assays based on natural LPC. Here we report the development of ultrafast mass spectrometry–based ATX assays enabling the measurement of data points within 13 s, which is 10 times faster than classic liquid chromatography–mass spectrometry. To this end, we set up a novel in vitro and whole-blood assay. We demonstrate that the potencies determined with these assays are in good agreement with the in vivo efficacy and that the whole-blood assay has the best predictive power. This high-throughput label-free approach paired with the translatable data quality is highly attractive for appropriate guidance of medicinal chemists for constructing strong structure-activity relationships.

scholarly journals High-Throughput, Label-Free Isolation of White Blood Cells From Whole Blood Using Parallel Spiral Microchannels With U-Shaped Cross-Section

2021 ◽  
Author(s):  
Amirhossein Mehran ◽  
Peyman Rostami ◽  
Mohammad Said Saidi ◽  
Bahar Firoozabadi ◽  
Navid Kashaninejad
Keyword(s):  
Cross Section ◽  
High Throughput ◽  
Whole Blood ◽  
Blood Cells ◽  
High Efficiency ◽  
Rectangular Cross Section ◽  
White Blood Cells ◽  
Label Free ◽  
Entire Cross Section ◽  
Shaped Cross Section

Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner cross-section of the microchannel by moving smaller blood cells (e.g., red blood cells (RBCs) and platelets) to the outer microchannel section and preventing them from returning to the inner microchannel section. Therefore, it overcomes the major limitation of a rectangular cross-section where secondary Dean vortices constantly enforce particles throughout the entire cross-section and decrease its isolation efficiency. Under optimal settings, more than 95% of WBCs can be isolated from whole blood under high-throughput (6 ml/min), high-purity (88%), and high-capacity (180 ml of sample in 1 hour) conditions. High efficiency, fast processing time, and non-invasive WBC isolation from large blood samples without centrifugation, RBC lysis, cell biomarkers, and chemical pre-treatments make this method an ideal choice for downstream cell study platforms.

On the Design of a DLD-DEP Device for Separation of Circulating Tumor Cells in Blood

2020 ◽  
Author(s):  
Mehdi Rahmati ◽  
Xiaolin Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Progression ◽  
High Throughput ◽  
Blood Cells ◽  
Lateral Displacement ◽  
Uniform Electric Field ◽  
Label Free ◽  
Particle Tracing ◽  
Key Factor

Abstract Circulating Tumor Cells (CTCs), which migrate from original sites in a body to distant organs through blood, are a key factor in cancer detection. Emerging Label-free techniques owing to their inherent advantage to preserve characteristics of sorted cells and low consumption of samples can be promising to the prediction of cancer progression and metastasis research. Deterministic Lateral Displacement (DLD) is one of the label-free separation techniques employing a specific arrangement of micro-posts for continuous separation of suspended cells in a buffer based on the size of cells. Separation based solely on size is challenging since the size distributions of CTCs might overlap with those of normal blood cells. To address this problem, DLD can be combined with dielectrophoresis (DEP) technique which is the phenomenon of particle movement in a non-uniform electric field owing to the polarization effect. Although, DLD devices employ the laminar flow in low Reynolds number (Re) fluid flow due to predictability of such flow regimes, they should be improved to work in higher Re flow regime so as to attain high throughput devices. In this paper, a particle tracing simulation is developed to study the effects of different post shapes, shift fraction of micropost arrays, and dielectrophoresis forces on separation of CTCs from peripheral blood cells. Our numerical model and results provide a groundwork for design and fabrication of high-throughput DLD-DEP devices for improvement of CTC separation.

Acoustofluidic, Label-Free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells

2015 ◽  
Vol 87 (18) ◽  
pp. 9322-9328 ◽  
Author(s):  
Maria Antfolk ◽  
Cecilia Magnusson ◽  
Per Augustsson ◽  
Hans Lilja ◽  
Thomas Laurell
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Rare Tumor ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document