Isolation of circulating fetal trophoblasts by a four-stage inertial microfluidic device for single-cell analysis and noninvasive prenatal testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (23) ◽  
pp. 4342-4348
Author(s):  
Yifang Huang ◽  
Sheng Yu ◽  
Shuzhe Chao ◽  
Limei Wu ◽  
Maliang Tao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Whole Blood ◽  
Single Cell Analysis ◽  
Low Cost ◽  
Prenatal Testing ◽  
Cell Analysis ◽  
Noninvasive Prenatal Testing ◽  
Blood Samples ◽  
Trophoblastic Cells ◽  
Whole Blood Samples

A novel four-stage inertial microfluidic chip is developed for isolating rare circulating trophoblastic cells from whole blood samples of pregnancies. The antibody-free, low-cost assay may serve as a useful platform for noninvasive prenatal testing.

scholarly journals Mobile Device for Disease Diagnosis and Data Tracking in Resource-Limited Settings

2013 ◽  
Vol 59 (4) ◽  
pp. 629-640 ◽  
Author(s):  
Curtis D Chin ◽  
Yuk Kee Cheung ◽  
Tassaneewan Laksanasopin ◽  
Mario M Modena ◽  
Sau Yin Chin ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Mobile Device ◽  
Whole Blood ◽  
Low Cost ◽  
Resource Limited Settings ◽  
Health Records ◽  
Blood Samples ◽  
Resource Limited ◽  
Whole Blood Samples ◽  
Diagnostic Sensitivity And Specificity

BACKGROUND Collection of epidemiological data and care of patients are hampered by lack of access to laboratory diagnostic equipment and patients' health records in resource-limited settings. We engineered a low-cost mobile device that combines cell-phone and satellite communication technologies with fluid miniaturization techniques for performing all essential ELISA functions. METHODS We assessed the device's ability to perform HIV serodiagnostic testing in Rwanda and synchronize results in real time with electronic health records. We tested serum, plasma, and whole blood samples collected in Rwanda and on a commercially available sample panel made of mixed antibody titers. RESULTS HIV testing on 167 Rwandan patients evaluated for HIV, viral hepatitis, and sexually transmitted infections yielded diagnostic sensitivity and specificity of 100% and 99%, respectively. Testing on 40 Rwandan whole-blood samples—using 1 μL of sample per patient—resulted in diagnostic sensitivity and specificity of 100% and 100%. The mobile device also successfully transmitted all whole-blood test results from a Rwandan clinic to a medical records database stored on the cloud. For all samples in the commercial panel, the device produced results in agreement with a leading ELISA test, including detection of weakly positive samples that were missed by existing rapid tests. The device operated autonomously with minimal user input, produced each result 10 times faster than benchtop ELISA, and consumed as little power as a mobile phone. CONCLUSIONS A low-cost mobile device can perform a blood-based HIV serodiagnostic test with laboratory-level accuracy and real-time synchronization of patient health record data.

Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

The Analyst ◽  
2018 ◽  
Vol 143 (2) ◽  
pp. 503-510 ◽  
Author(s):  
Meng-Zhe Tsai ◽  
Chan-Te Hsiung ◽  
Yang Chen ◽  
Cheng-Sheng Huang ◽  
Hsin-Yun Hsu ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Chip ◽  
Whole Blood ◽  
Lab On A Chip ◽  
Sample Pretreatment ◽  
Label Free ◽  
Blood Samples ◽  
Whole Blood Samples

We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

Detection of melanoma cells in whole blood samples using spectral imaging and optical clearing

2020 ◽  
pp. 1-1
Author(s):  
Polina A. Dyachenko Timoshina ◽  
Leonid E. Dolotov ◽  
Ekaterina N. Lazareva ◽  
Anastasiia A. Kozlova ◽  
Olga A. Inozemtseva ◽  
...  
Keyword(s):  
Whole Blood ◽  
Spectral Imaging ◽  
Melanoma Cells ◽  
Blood Samples ◽  
Optical Clearing ◽  
Whole Blood Samples
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