Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

The Analyst ◽  
2018 ◽  
Vol 143 (2) ◽  
pp. 503-510 ◽  
Author(s):  
Meng-Zhe Tsai ◽  
Chan-Te Hsiung ◽  
Yang Chen ◽  
Cheng-Sheng Huang ◽  
Hsin-Yun Hsu ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Chip ◽  
Whole Blood ◽  
Lab On A Chip ◽  
Sample Pretreatment ◽  
Label Free ◽  
Blood Samples ◽  
Whole Blood Samples

We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor.

Isolation of circulating fetal trophoblasts by a four-stage inertial microfluidic device for single-cell analysis and noninvasive prenatal testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (23) ◽  
pp. 4342-4348
Author(s):  
Yifang Huang ◽  
Sheng Yu ◽  
Shuzhe Chao ◽  
Limei Wu ◽  
Maliang Tao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Whole Blood ◽  
Single Cell Analysis ◽  
Low Cost ◽  
Prenatal Testing ◽  
Cell Analysis ◽  
Noninvasive Prenatal Testing ◽  
Blood Samples ◽  
Trophoblastic Cells ◽  
Whole Blood Samples

A novel four-stage inertial microfluidic chip is developed for isolating rare circulating trophoblastic cells from whole blood samples of pregnancies. The antibody-free, low-cost assay may serve as a useful platform for noninvasive prenatal testing.

Surface acoustic wave immunosensor for real-time detection of hepatitis B surface antibodies in whole blood samples

2009 ◽  
Vol 24 (10) ◽  
pp. 3120-3125 ◽  
Author(s):  
Hun Joo Lee ◽  
Kak Namkoong ◽  
Eun Chol Cho ◽  
Christopher Ko ◽  
Jae Chan Park ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Acoustic Wave ◽  
Real Time ◽  
Surface Acoustic Wave ◽  
Whole Blood ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Real Time Detection ◽  
Surface Antibodies

scholarly journals Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Paolo Gaibani ◽  
Mara Mariconti ◽  
Gloria Bua ◽  
Sonia Bonora ◽  
Davide Sassera ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Extraction Method ◽  
Sample Type ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Rdna Gene ◽  
Whole Blood Samples ◽  
Pcr Targeting

Molecular methods are important tools in the diagnosis of bloodstream bacterial infections, in particular in patients treated with antimicrobial therapy, due to their quick turn-around time. Here we describe a new broad-range real-time PCR targeting the 23S rDNA gene and capable to detect as low as 10 plasmid copies per reaction of targeted bacterial 23S rDNA gene. Two commercially available DNA extraction kits were evaluated to assess their efficiency for the extraction of plasma and whole blood samples spiked with different amount of eitherStaphylococcus aureusorEscherichia coli, in order to find the optimal extraction method to be used. Manual QIAmp extraction method with enzyme pre-treatment resulted the most sensitive for detection of bacterial load. Sensitivity of this novel assay ranged between 10 and 103 CFU per PCR reaction forE. coliandS. aureusin human whole blood samples depending on the extraction methods used. Analysis of plasma samples showed a 10- to 100-fold reduction of bacterial 23S rDNA in comparison to the corresponding whole blood specimens, thus indicating that whole blood is the preferential sample type to be used in this real-time PCR protocol. Our results thus show that the 23S rDNA gene represents an optimal target for bacteria quantification in human whole blood.

Evaluation of an automated and highly sensitive real-time PCR assay for CMV DNA in whole blood samples

2006 ◽  
Vol 36 ◽  
pp. S26
Author(s):  
C. Deback ◽  
S. Akhavan ◽  
S. Blanc-Perrel ◽  
F. Dupuis ◽  
S. Schaffer ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Pcr Assay ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Whole Blood Samples

PX-13 Detection and quantitation of cytomegalovirus(CMV)DNA in EDTA whole blood samples by automated extraction and real-time PCR

2009 ◽  
Vol 46 ◽  
pp. S50
Author(s):  
B.D.A. Michelin ◽  
M. Bozic ◽  
S. Hammerschmidt ◽  
C. Homberg ◽  
D. Violan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Automated Extraction ◽  
Blood Samples ◽  
Whole Blood Samples

A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples

2007 ◽  
Vol 197 (3) ◽  
pp. 313-324 ◽  
Author(s):  
Lutz Eric Lehmann ◽  
Klaus-Peter Hunfeld ◽  
Thomas Emrich ◽  
Gerd Haberhausen ◽  
Heimo Wissing ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Rapid Detection ◽  
Fungal Pathogens ◽  
Pcr Assay ◽  
Blood Samples ◽  
Whole Blood Samples
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