Towards practical sample preparation in point-of-care testing: user-friendly microfluidic devices

Lab on a Chip ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1191-1203 ◽  
Author(s):  
Juhwan Park ◽  
Dong Hyun Han ◽  
Je-Kyun Park
Keyword(s):  
Sample Preparation ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Future Perspectives ◽  
Point Of Care Testing ◽  
User Friendly

An overview and future perspectives of user-friendly microfluidic devices have been reported in terms of practical sample preparation in point-of-care testing.

scholarly journals Disposable Voltammetric Immunosensors Integrated with Microfluidic Platforms for Biomedical, Agricultural and Food Analyses: A Review

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4124 ◽  
Author(s):  
Fabiana Felix ◽  
Alexandre Baccaro ◽  
Lúcio Angnes
Keyword(s):  
Quality Management ◽  
Signal Amplification ◽  
Screen Printing ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Strong Interactions ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Broad Variety ◽  
Interesting Alternative

Disposable immunosensors are analytical devices used for the quantification of a broad variety of analytes in different areas such as clinical, environmental, agricultural and food quality management. They detect the analytes by means of the strong interactions between antibodies and antigens, which provide concentration-dependent signals. For the herein highlighted voltammetric immunosensors, the analytical measurements are due to changes in the electrical signals on the surface of the transducers. The possibility of using disposable and miniaturized immunoassays is a very interesting alternative for voltammetric analyses, mainly, when associated with screen-printing technologies (screen-printed electrodes, SPEs), and microfluidic platforms. The aim of this paper is to discuss a carefully selected literature about different examples of SPEs-based immunosensors associated with microfluidic technologies for diseases, food, agricultural and environmental analysis. Technological aspects of the development of the voltammetric immunoassays such as the signal amplification, construction of paper-based microfluidic platforms and the utilization of microfluidic devices for point-of-care testing will be presented as well.

scholarly journals Point-of-Care Testing-the Key in the Battle against SARS-CoV-2 Pandemic

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1464
Author(s):  
Florina Silvia Iliescu ◽  
Ana Maria Ionescu ◽  
Larisa Gogianu ◽  
Monica Simion ◽  
Violeta Dediu ◽  
...  
Keyword(s):  
Clinical Decision Making ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Testing ◽  
Clinical Decision ◽  
Rapid Screening ◽  
Diagnostic Tools ◽  
Point Of Care Testing ◽  
Qrt Pcr ◽  
User Friendly

The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the “gold standard” involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.

scholarly journals Elasto-Magnetic Pumps Integrated within Microfluidic Devices

2021 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Jacob L. Binsley ◽  
Elizabeth L. Martin ◽  
Thomas O. Myers ◽  
Stefano Pagliara ◽  
Feodor Y. Ogrin
Keyword(s):  
Magnetic System ◽  
Point Of Care ◽  
Microfluidic Channel ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Point Of Care Testing ◽  
Ongoing Research ◽  
Flow Rates ◽  
Pumping System ◽  
Oscillating Magnetic Field

Many lab-on-a-chip devices require a connection to an external pumping system in order to perform their function. While this is not problematic in typical laboratory environments, it is not always practical when applied to point-of-care testing, which is best utilized outside of the laboratory. Therefore, there has been a large amount of ongoing research into producing integrated microfluidic components capable of generating effective fluid flow from on-board the device. This research aims to introduce a system that can produce practical flow rates, and be easily fabricated and actuated using readily available techniques and materials. We show how an asymmetric elasto-magnetic system, inspired by Purcell’s three-link swimmer, can provide this solution through the generation of non-reciprocal motion in an enclosed environment. The device is fabricated monolithically within a microfluidic channel at the time of manufacture, and is actuated using a weak, oscillating magnetic field. The flow rate can be altered dynamically, and the direction of the resultant flow can be controlled by adjusting the frequency of the driving field. The device has been proven, experimentally and numerically, to operate effectively when applied to fluids with a range of viscosities. Such a device may be able to replace external pumping systems in portable applications.

scholarly journals Microfluidic devices powered by integrated elasto-magnetic pumps

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4285-4295
Author(s):  
Jacob L. Binsley ◽  
Elizabeth L. Martin ◽  
Thomas O. Myers ◽  
Stefano Pagliara ◽  
Feodor Y. Ogrin
Keyword(s):  
Point Of Care ◽  
Microfluidic Devices ◽  
Point Of Care Testing

Integrated elasto-magnetic pumps power portable microfluidic devices for point of care testing.

Integrated paper-based microfluidic devices for point-of-care testing

2018 ◽  
Vol 10 (29) ◽  
pp. 3567-3581 ◽  
Author(s):  
Tian Tian ◽  
Yunpeng Bi ◽  
Xing Xu ◽  
Zhi Zhu ◽  
Chaoyong Yang
Keyword(s):  
Signal Transduction ◽  
Point Of Care ◽  
Sample Pretreatment ◽  
Microfluidic Devices ◽  
Point Of Care Testing

This review highlights the developments, accomplishments and challenges of integrated μPADs, including sample pretreatment, signal transduction/amplification and results output.

Integrating Target-Responsive Hydrogel with Pressuremeter Readout Enables Simple, Sensitive, User-Friendly, Quantitative Point-of-Care Testing

2017 ◽  
Vol 9 (27) ◽  
pp. 22252-22258 ◽  
Author(s):  
Dan Liu ◽  
Shasha Jia ◽  
Huimin Zhang ◽  
Yanli Ma ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
User Friendly

A Distance-based Capillary Biosensor Using Wettability Alteration

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yansheng Li ◽  
Xiujin Men ◽  
Guowei Gao ◽  
Ye Tian ◽  
Yongqiang Wen ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Wettability Alteration ◽  
Detection Methods ◽  
Point Of Care Testing ◽  
User Friendly

Distance-based detection methods with quantitative readout are of great significance to point-of-care testing (POCT), which are low-cost, user-friendly and can be integrated into portable analytical devices. Here, we submit a...

scholarly journals Portable RT-PCR system: A Rapid and Scalable Diagnostic Tool for COVID-19 Testing

2021 ◽  
Author(s):  
Hosam M Zowawi ◽  
Thamer H Alenazi ◽  
Waleed S AlOmaim ◽  
Ahmad Wazzan ◽  
Abdullah Alsufayan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Rapid Test ◽  
Standard Of Care ◽  
Test Results ◽  
Rt Pcr ◽  
Point Of Care Testing ◽  
System A ◽  
Portable System ◽  
Remote Sites ◽  
User Friendly

Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on RT-PCR, which is routinely performed in well-equipped laboratories by trained professionals at specific locations. However, during busy periods high numbers of samples queued for testing can delay the test results, impacting upon efforts to reduce the infection risk. Besides, the absence of well-established laboratories at remote sites and low-resourced environments can contribute to a silent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These reasons compel the need to accommodate point-of-care testing for COVID-19 that meets the ASSURED criteria (Affordable, Sensitive, Specific, User-friendly, Rapid, robust, Equipment-free, and Deliverable). This study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests. Nasopharyngeal and nasal swabs were used. Of the 192 samples tested using the Biomeme SARS-CoV-2 system, the results from 189 samples (98.4%) were in agreement with the reference standard-of-care RT-PCR testing for SARS-CoV-2. The portable system generated simultaneous results for nine samples in 80 mins with high positive and negative percent agreements of 99.0% and 97.8%, respectively. We performed separate testing in a sealed glove box, offering complete biosafety containment. Thus, the Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote and low-resourced settings. Single Sentence Summary The Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote sites and low-resourced settings.

scholarly journals Point-of-care testing (POCT): Current techniques and future perspectives

2011 ◽  
Vol 30 (6) ◽  
pp. 887-898 ◽  
Author(s):  
Peter B. Luppa ◽  
Carolin Müller ◽  
Alice Schlichtiger ◽  
Harald Schlebusch
Keyword(s):  
Point Of Care ◽  
Future Perspectives ◽  
Point Of Care Testing
Sign in / Sign up

Export Citation Format

Share Document