Protective effects of tryptophan-catabolizing Lactobacillus plantarum KLDS 1.0386 against dextran sodium sulfate-induced colitis in mice

2020 ◽  
Vol 11 (12) ◽  
pp. 10736-10747
Author(s):  
Jialu Shi ◽  
Peng Du ◽  
Qinggang Xie ◽  
Nana Wang ◽  
Huizhen Li ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Protective Effects ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Indole 3 Acetic Acid

L. plantarum KLDS 1.0386 combined with tryptophan alleviates ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) by increasing the level of indole-3-acetic acid (IAA), stimulating the AHR/IL-22/STAT3 signaling pathway and regulating gut microbiota in mice.

Effects of a β-type glycosidic polysaccharide from Flammulina velutipes on anti-inflammation and gut microbiota modulation in colitis mice

2020 ◽  
Vol 11 (5) ◽  
pp. 4259-4274
Author(s):  
Ruiqiu Zhao ◽  
Yang Ji ◽  
Xin Chen ◽  
Anxiang Su ◽  
Gaoxing Ma ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gut Microbiota ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Flammulina Velutipes ◽  
Anti Inflammation

Using the Flammulina velutipes polysaccharide (FVP) extracted from our previous study, herein, we investigated the improvement of this β-type glycosidic polysaccharide in alleviating dextran sodium sulfate-induced ulcerative colitis (UC) in mice.

scholarly journals Effect of Triptolide on Dextran Sodium Sulfate-Induced Ulcerative Colitis and Gut Microbiota in Mice

2020 ◽  
Vol 10 ◽  
Author(s):  
Hao Wu ◽  
Quan Rao ◽  
Guang-Chao Ma ◽  
Xiao-Hong Yu ◽  
Cong-En Zhang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gut Microbiota ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate

scholarly journals MiR-223 improves intestinal inflammation through inhibiting the IL-6/STAT3 signaling pathway in dextran sodium sulfate-induced experimental colitis

2020 ◽  
Author(s):  
Juanjuan Zhang ◽  
Chenyang Wang ◽  
Zhen Guo ◽  
Binlin Da ◽  
Weiming Zhu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Sodium Sulfate ◽  
Quantitative Polymerase Chain Reaction ◽  
Negative Control ◽  
Dextran Sodium Sulfate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Colonic Inflammation ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Tnf Α

Abstract Background: The pathogenesis of inflammatory bowel disease (IBD) has not yet been clarified and is closely related to several proinflammatory factors. MicroRNA-233 (miR-223) might be involved in the development of IBD; however, the mechanism underlying its pathogenesis is unclear. In this study, we attempted to determine the role of miR-223 in dextran sodium sulfate (DSS)-induced colitis and explore the involvement of the IL-6/STAT3 pathway in the development of intestinal mucosal inflammation.Methods: Male C57BL/6 mice were divided into six groups: control (WT) group, DSS group, DSS+miR-223 agomir (DSS+A) group, DSS+miR-223 agomir negative control (DSS+A+NC) group, DSS+miR-223 antagomir (DSS+AN) group and DSS+miR-223 antagomir negative control (DSS+AN+NC) group. Body weight, stool consistency, fecal blood and the disease activity index (DAI) score were recorded daily. The length of each colon was measured, and colonic inflammation was evaluated with hematoxylin and eosin (H&E) staining and histopathological scoring. The expression of myeloperoxidase (MPO), TNF-α, IL-6, IL-10 and IL-17 in the colonic tissues was measured by Enzyme-linked Immunosorbent Assay (ELISA) and real-time quantitative polymerase chain reaction (RT-qPCR). The mRNA expression of gp130, Bcl-2 and Bcl-xl in the colon was measured using RT-qPCR. The colonic levels of STAT3 and p-STAT3 were determined by Western blotting.Results: MiR-223 expression in the terminal ileum and colon was increased in the DSS group compared with the WT group. Colitis symptoms were significantly alleviated in the DSS+A group and exacerbated in the DSS+AN group after administration of the miR-223 agomir and antagomir, respectively. MPO, TNF-α, IL-6 and IL-17 were decreased and IL-10 was increased in the DSS+A group, but these changes were reversed in the DSS+AN group. Gp130 mRNA, p-STAT3, Bcl-2 and Bcl-xl in the colon declined in the DSS+A group, but these levels increased in the DSS+AN group.Conclusion: The upregulation of miR-223 by agomir administration alleviated colonic inflammation in a DSS-induced colitis model, which was likely mediated by inhibiting the production of proinflammatory cytokines via the IL-6/STAT3 signaling pathway. These findings provide evidence that miR-223 might have potential therapeutic implications in IBD.

scholarly journals Protective effects of fermented rice extract on ulcerative colitis induced by dextran sodium sulfate in mice

2020 ◽  
Vol 8 (3) ◽  
pp. 1718-1728 ◽  
Author(s):  
Won‐Seok Oh ◽  
Jae‐Chul Jung ◽  
Yong‐Min Choi ◽  
Ju‐Young Mun ◽  
Sae‐Kwang Ku ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Protective Effects

Gut microbiota modulation and anti-inflammatory properties of mixed lactobacilli in dextran sodium sulfate-induced colitis in mice

2021 ◽  
Author(s):  
Jialu Shi ◽  
Qing gang Xie ◽  
Yingxue Yue ◽  
Qingxue Chen ◽  
Lina Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Lactobacillus Helveticus ◽  
Immunomodulatory Activity ◽  
Anti Inflammatory

Lactobacillus acidophilus KDSL 1.0901，Lactobacillus helveticus KDSL 1.8701and Lactobacillus plantarum KDSL 1.0318 showed antibacterial activity, antioxidant activity and immunomodulatory activity. In the current study, we evaluated the effects of three tested...

scholarly journals Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

2018 ◽  
Vol 51 (1) ◽  
pp. 441-451 ◽  
Author(s):  
Gang Liu ◽  
Wenxin Yan ◽  
Sujuan  Ding ◽  
Hongmei Jiang ◽  
Yong Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Gut Microbiota ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Daily Weight Gain ◽  
Control Group ◽  
Unrestricted Access ◽  
Hematoxylin And Eosin ◽  
Colitis Model

Background/Aims: There are known links between inflammatory bowel disease (IBD) and changes in the microbiota of the gut and inflammation and oxidative stress. In this study, a colitis model induced by dextran sodium sulfate (DSS) in mice is used to evaluate whether the presence of bioactive peptides IRW (Ile-Arg-Trp) and IQW (Ile-Gln-Trp) peptides is advantageous. Methods: The mice were arbitrarily assigned to the following four groups: (i) control (untreated), (ii) dextran sodium sulfate (DSS) treated, (iii) IRW-DSS treated, and (iv) IQW-DSS treated. For 7 days, the control group subjects had unrestricted access to untreated drinking water, whereas the drinking water supplied to the subjects in the DSS, IRW-DSS, and IQW-DSS groups during this period consisted of 5% DSS solution. The colonic lesions were scored after hematoxylin and eosin staining. Serum antioxidant capacity was analyzed by 2,2’-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical cation decolorization test and the microbiota in the colonic contents were sequenced by HiSeq2500 PE250. Results: The presence of DSS reduced daily weight gain, enhanced histopathology scores, and inhibited antioxidant enzyme expression. Superoxide dismutase, catalase, and glutathione peroxidase activities in the DSS-induced colitis model were significantly enhanced (P < 0.05) in the presence of dietary IRW and IQW. Furthermore, the Simpson index was significantly increased (P < 0.05) in the presence of dietary IRW and IQW compared to the control group. IRW and IQW increased the abundance of Coprococcus_1, Ruminococcaceae_UCG-014, and Desulfovibrio compared to the control group and DSS group. Furthermore, IQW decreased the abundance of Bacteroides in relation to the control group, but increased Parabacteroides. In addition, IRW increased the level of Anaerotruncus, Oscillibacter, and Ruminiclostridium_9 compared to the control group. Conclusion: This study concludes that the presence of IRW or IQW can mitigate DSS-induced oxidative stress by improving the activities of antioxidant enzymes, increasing intestinal microbial diversity and enhancing the abundance of gut microbiota, which may help maintain the homeostasis of host health and microenvironment in a DSS-induced mouse model, thus providing a potential further treatment for IBD patients.

The Role of SPARC in Human Ulcerative Colitis and Murine Dextran Sodium Sulfate-Induced Colitis

2011 ◽  
Vol 140 (5) ◽  
pp. S-837
Author(s):  
Tomohisa Takagi ◽  
Yuji Naito ◽  
Wataru Aoi ◽  
Ryusuke Horie ◽  
Kazuhiko Uchiyama ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate
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