scholarly journals miR‐223 improves intestinal inflammation through inhibiting the IL‐6/STAT3 signaling pathway in dextran sodium sulfate‐induced experimental colitis

2020 ◽  
Author(s):  
Juanjuan Zhang ◽  
Chenyang Wang ◽  
Zhen Guo ◽  
Binlin Da ◽  
Weiming Zhu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Sodium Sulfate ◽  
Intestinal Inflammation ◽  
Experimental Colitis ◽  
Dextran Sodium Sulfate ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway

scholarly journals MiR-223 improves intestinal inflammation through inhibiting the IL-6/STAT3 signaling pathway in dextran sodium sulfate-induced experimental colitis

2020 ◽  
Author(s):  
Juanjuan Zhang ◽  
Chenyang Wang ◽  
Zhen Guo ◽  
Binlin Da ◽  
Weiming Zhu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Sodium Sulfate ◽  
Quantitative Polymerase Chain Reaction ◽  
Negative Control ◽  
Dextran Sodium Sulfate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Colonic Inflammation ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Tnf Α

Abstract Background: The pathogenesis of inflammatory bowel disease (IBD) has not yet been clarified and is closely related to several proinflammatory factors. MicroRNA-233 (miR-223) might be involved in the development of IBD; however, the mechanism underlying its pathogenesis is unclear. In this study, we attempted to determine the role of miR-223 in dextran sodium sulfate (DSS)-induced colitis and explore the involvement of the IL-6/STAT3 pathway in the development of intestinal mucosal inflammation.Methods: Male C57BL/6 mice were divided into six groups: control (WT) group, DSS group, DSS+miR-223 agomir (DSS+A) group, DSS+miR-223 agomir negative control (DSS+A+NC) group, DSS+miR-223 antagomir (DSS+AN) group and DSS+miR-223 antagomir negative control (DSS+AN+NC) group. Body weight, stool consistency, fecal blood and the disease activity index (DAI) score were recorded daily. The length of each colon was measured, and colonic inflammation was evaluated with hematoxylin and eosin (H&E) staining and histopathological scoring. The expression of myeloperoxidase (MPO), TNF-α, IL-6, IL-10 and IL-17 in the colonic tissues was measured by Enzyme-linked Immunosorbent Assay (ELISA) and real-time quantitative polymerase chain reaction (RT-qPCR). The mRNA expression of gp130, Bcl-2 and Bcl-xl in the colon was measured using RT-qPCR. The colonic levels of STAT3 and p-STAT3 were determined by Western blotting.Results: MiR-223 expression in the terminal ileum and colon was increased in the DSS group compared with the WT group. Colitis symptoms were significantly alleviated in the DSS+A group and exacerbated in the DSS+AN group after administration of the miR-223 agomir and antagomir, respectively. MPO, TNF-α, IL-6 and IL-17 were decreased and IL-10 was increased in the DSS+A group, but these changes were reversed in the DSS+AN group. Gp130 mRNA, p-STAT3, Bcl-2 and Bcl-xl in the colon declined in the DSS+A group, but these levels increased in the DSS+AN group.Conclusion: The upregulation of miR-223 by agomir administration alleviated colonic inflammation in a DSS-induced colitis model, which was likely mediated by inhibiting the production of proinflammatory cytokines via the IL-6/STAT3 signaling pathway. These findings provide evidence that miR-223 might have potential therapeutic implications in IBD.

scholarly journals HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway

2019 ◽  
Vol 20 (5) ◽  
pp. 1069 ◽  
Author(s):  
Song Deng ◽  
Aiping Wang ◽  
Xi Chen ◽  
Qun Du ◽  
Yanli Wu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Intestinal Inflammation ◽  
Underlying Mechanism ◽  
Peptic Ulcers ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Hct 116 ◽  
Chronic Intestinal Inflammation

Colitis-associated cancer (CAC) is a malignant disease of the colon that is caused by recurrent episodes of chronic intestinal inflammation. Huangqi Baizhu decoction (HBD) is a classic prescription comprised of Radix Astragali and Rhizoma Atractylodis, which are usually used to treat digestive conditions, such as peptic ulcers, colitis, or colorectal carcinoma in clinics. HBD is well known for “tonifying qi and spleen” based on the theories of traditional Chinese medicine, and has the preponderant effect of alleviating chronic intestinal mucosa damage associated with disease. However, the underlying mechanism behind this is still unknown. In the current study, we employed the AOM/DSS mouse model to analyze the effects of HBD on the development of inflammation in colonic carcinoma. The in vivo study showed that HBD could significantly reduce the mortality of mice and control the incidence and size of colonic tumors by inhibiting the IL-6/STAT3 signaling pathway. In vitro, Astragaloside and Atractylenolide (CAA), the main components of HBD, inhibited the proliferation of HCT-116 cells as determined by an MTT assay. Furthermore, CAA notably suppressed the protein expression of IL-6R, STAT3, Survivin, and Cyclin D1 induced by IL-6 in HCT-116 and RAW264.7 cells. These results suggested that HBD exhibits anti-inflammatory and anti-proliferative effects, inhibiting the development of CAC in mice.

Lactobacillus paracasei L9 improves colitis by expanding butyrate-producing bacteria that inhibits the IL-6/STAT3 signaling pathway

2021 ◽  
Author(s):  
Zhengquan Yu ◽  
Min Deng ◽  
Xi Wu ◽  
Xiaoyue Duan ◽  
Jiuzhi Xu ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Signaling Pathway ◽  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Lactobacillus Paracasei ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Inflammatory Bowel ◽  
Chronic Intestinal Inflammation

Inflammatory bowel disease (IBD) is a chronic intestinal inflammation that is currently incurable. Increasing evidences indicate that supplementation with probiotics could improve the symptoms of IBD. It is scientifically significant...

Protective effects of tryptophan-catabolizing Lactobacillus plantarum KLDS 1.0386 against dextran sodium sulfate-induced colitis in mice

2020 ◽  
Vol 11 (12) ◽  
pp. 10736-10747
Author(s):  
Jialu Shi ◽  
Peng Du ◽  
Qinggang Xie ◽  
Nana Wang ◽  
Huizhen Li ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Protective Effects ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Indole 3 Acetic Acid

L. plantarum KLDS 1.0386 combined with tryptophan alleviates ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) by increasing the level of indole-3-acetic acid (IAA), stimulating the AHR/IL-22/STAT3 signaling pathway and regulating gut microbiota in mice.

scholarly journals Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus

Theranostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 4078-4089
Author(s):  
Na-Na Yang ◽  
Jing-Wen Yang ◽  
Yang Ye ◽  
Jin Huang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Postoperative Ileus ◽  
Intestinal Inflammation ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway
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