The development of lentil derived protein–iron complexes and their effects on iron deficiency anemia in vitro

2020 ◽  
Vol 11 (5) ◽  
pp. 4185-4192 ◽  
Author(s):  
Ezgi Evcan ◽  
Sukru Gulec
Keyword(s):  
Iron Deficiency ◽  
Functional Properties ◽  
Iron Deficiency Anemia ◽  
Iron Complexes ◽  
Deficiency Anemia ◽  
Mrna Levels ◽  
Iron Minerals

Lentil derived proteins have the capacity to chelate iron minerals and hydrolysed protein–iron complexes have functional properties on iron deficiency anemia in in vitro by influencing mRNA levels of iron regulating genes.

In vitro cytokine production in patients with iron deficiency anemia

2004 ◽  
Vol 113 (3) ◽  
pp. 340-344 ◽  
Author(s):  
Michael Bergman ◽  
Hanna Bessler ◽  
Hertzel Salman ◽  
Dimitri Siomin ◽  
Rachel Straussberg ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Cytokine Production ◽  
Deficiency Anemia

Iron Deficiency Anemia: Recovery from in vitro Oxidative Stress

1993 ◽  
Vol 90 (2) ◽  
pp. 94-98 ◽  
Author(s):  
M. Bartal ◽  
D. Mazor ◽  
A. Dvilansky ◽  
N. Meyerstein
Keyword(s):  
Oxidative Stress ◽  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Deficiency Anemia

scholarly journals MÖSSBAUER SPECTROSCOPY OF FERROUS FUMARATE IN PHARMACEUTICAL PRODUCT USED TO TREAT ANEMIA

2020 ◽  
Vol 21 (3) ◽  
pp. 43-49
Author(s):  
F.G. Vagizov ◽  
◽  
J. Nicolas Pineda M. ◽  
Keyword(s):  
Iron Deficiency ◽  
Isomer Shift ◽  
Debye Temperature ◽  
Quadrupole Splitting ◽  
Iron Deficiency Anemia ◽  
Public Health Problem ◽  
World Health ◽  
Deficiency Anemia ◽  
Mössbauer Measurements

The World Health Organization (WHO) considers iron deficiency anemia a serious public health problem in developing countries and recommends the use of iron tablets containing iron II for prevention and treatment. The results of Mössbauer measurements of the drug “Ferretab”, which is widely used in medicine for the treatment and prevention of iron deficiency anemia, are presented. This drug contains fumarate iron, C4H2FeO4, and has a small amount of folic acid. In this paper, the temperature dependence of isomer shift and quadrupole splitting values of 57Fe nuclei in iron fumarate were studied. The measurements show that when the temperature increases, the isomer shift and quadrupole splitting of 57Fe nuclei in iron fumarate decreases, the decrease in the isomer shift value is associated with the second-order Doppler effect. Based on Mössbauer measurements, the Debye temperature of this drug was determined. The Debye temperature gives us information about the strong bonding of 57Fe atoms with the environment. A high temperature value means a strong bond and vice versa, a small temperature value means a bond with low rigidity. The coupling constant (Debye temperature) defined for “Ferretab” iron nuclei has been compared with different Debye temperatures found in previous studies on some iron deficiency anemia drugs. Additionally, the values were compared with various clinical studies conducted in in-vivo and in-vitro for comparison of the efficacy of some of the most commonly used drugs to treat and prevent iron deficiency anemia. According to these comparisons, it was established a possible relationship between the Debye temperature of the iron atoms of the drugs under study and their effectiveness. It was noted that the lower the Debye temperature of iron atoms of the drug, the more iron absorbs the human body.

scholarly journals Down-regulation of Bmp/Smad signaling by Tmprss6 is required for maintenance of systemic iron homeostasis

Blood ◽  
2010 ◽  
Vol 115 (18) ◽  
pp. 3817-3826 ◽  
Author(s):  
Karin E. Finberg ◽  
Rebecca L. Whittlesey ◽  
Mark D. Fleming ◽  
Nancy C. Andrews
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Iron Homeostasis ◽  
Intravenous Iron ◽  
Deficiency Anemia ◽  
Mrna Levels ◽  
Loss Of Function ◽  
Down Regulation ◽  
Smad Signaling ◽  
Hepcidin Expression

Abstract Iron-refractory, iron-deficiency anemia (IRIDA) is a familial disorder characterized by iron deficiency anemia unresponsive to oral iron treatment but partially responsive to intravenous iron therapy. Previously, we showed that IRIDA patients harbor loss-of-function mutations in TMPRSS6, a type II transmembrane serine protease primarily expressed by the liver. Both humans and mice with TMPRSS6 mutations show inappropriately elevated levels of the iron-regulatory hormone hepcidin, suggesting that TMPRSS6 acts to negatively regulate hepcidin expression. Here we investigate the relationship between Tmprss6 and the bone morphogenetic protein (BMP)–Smad signaling pathway, a key pathway promoting hepcidin transcription in hepatocytes. We show that livers from mice deficient for Tmprss6 have decreased iron stores and decreased Bmp6 mRNA, but markedly increased mRNA for Id1, a target gene of Bmp6 signaling. In contrast, mice deficient for both Tmprss6 and hemojuvelin (Hjv), a BMP coreceptor that augments hepcidin expression in hepatocytes, showed markedly decreased hepatic levels of hepcidin and Id1 mRNA, markedly increased hepatic Bmp6 mRNA levels, and systemic iron overload similar to mice deficient for Hjv alone. These findings suggest that down-regulation of Bmp/Smad signaling by Tmprss6 is required for regulation of hepcidin expression and maintenance of systemic iron homeostasis.

scholarly journals Ankaferd Influences mRNA Expression of Iron-Regulated Genes During Iron-Deficiency Anemia

2017 ◽  
Vol 24 (6) ◽  
pp. 960-964 ◽  
Author(s):  
Afife Gulec ◽  
Sukru Gulec
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Messenger Rna ◽  
In Vitro Models ◽  
Ammonium Citrate ◽  
Deficiency Anemia ◽  
Ferric Ammonium Citrate ◽  
Marker Genes ◽  
Divalent Metal Transporter

Ankaferd Blood Stopper (ABS) comprises a mixture of plants and stops bleeding via forming a protein network by erythroid aggregation. Bleeding causes reduction of iron levels in body. It has been indicated that ABS contains significant amount of iron. Thus, we investigated the biological activity of ABS-derived iron on iron-regulated genes during iron-deficiency anemia (IDA). IDA We selected Caco-2 and HepG2 cell lines as in vitro models of human intestine and liver, respectively. Iron deficiency anemia was induced by deferoxamine. The cells were treated with ferric ammonium citrate (FAC) and ABS. Messenger RNA levels of iron-regulated genes were analyzed by quantitative reverse transcription polymerase chain reaction to elucidate whether iron in ABS behaved similar to inorganic iron (FAC) during IDA. The results showed that ABS-derived iron influenced transcriptions of iron-regulated marker genes, including divalent metal transporter ( Dmt1), transferrin receptor ( TfR), ankyrin repeat domain 37 ( Ankrd37), and hepcidin ( Hamp) in IDA-induced Caco-2 and HepG2 cells. Our results suggest that when ABS is used to stop tissue bleeding, it might have an ability to reduce levels of IDA.

scholarly journals In Vitro and In Vivo Evaluations of Mesoporous Iron Particles for Iron Bioavailability

2019 ◽  
Vol 20 (21) ◽  
pp. 5291
Author(s):  
Lin ◽  
Wu ◽  
Liao ◽  
Jakfar ◽  
Tang ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Cell Model ◽  
Deficiency Anemia ◽  
Iron Particles ◽  
Standard Drug ◽  
Iron Deficient ◽  
Commercial Iron

Chronic renal failure involving hemodialysis results in blood loss during filtration. Iron deficiency and iron deficiency anemia can result. A compensatory increase in iron dosage has many side effects including discomfort. Elemental iron is a highly-pure iron source, which reduces the frequency of dosages; the solubility decreases with increased particle size or pore size. In this study, synthesized mesoporous iron particles (MIPs) were used to relieve iron deficiency anemia. Their bioavailability was measured in vitro by a Caco-2 cell model and in vivo in iron-deficient rats. In vitro bioavailability of MIPs was examined by measuring ferritin content in the Caco-2 cell model. Iron uptake of MIPs was significantly higher than commercial iron particles, which were less porous. In vivo bioavailability of MIPs was examined by measuring body weight gain and red blood cell-related parameters, compared with the bioavailability of standard drug ferrous sulfate in iron-deficient anemic rats. Finally, average hemoglobin content and hemoglobin regeneration efficiency were significantly higher in anemic rats supplemented with commercial iron particles, compared to anemic controls. In the 28-day oral toxicity test, MIPs were not significantly toxic to rat physiology or tissue histopathology. Thus, MIPs may allow effective recovery of hemoglobin in iron deficiency anemia.

scholarly journals Millets Can Have a Major Impact on Improving Iron Status, Hemoglobin Level, and in Reducing Iron Deficiency Anemia–A Systematic Review and Meta-Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Seetha Anitha ◽  
Joanna Kane-Potaka ◽  
Rosemary Botha ◽  
D. Ian Givens ◽  
Nur Liana Binti Sulaiman ◽  
...  
Keyword(s):  
Systematic Review ◽  
Iron Deficiency ◽  
Pearl Millet ◽  
Iron Deficiency Anemia ◽  
Iron Status ◽  
Ferritin Level ◽  
Meta Analysis ◽  
Hemoglobin Level ◽  
Deficiency Anemia

The prevalence of iron deficiency anemia is highest among low and middle-income countries. Millets, including sorghum, are a traditional staple in many of these countries and are known to be rich in iron. However, a wide variation in the iron composition of millets has been reported, which needs to be understood in consonance with its bioavailability and roles in reducing anemia. This systematic review and meta-analysis were carried out to analyze the scientific evidence on the bioavailability of iron in different types of millets, processing, and the impact of millet-based food on iron status and anemia. The results indicated that iron levels in the millets used to study iron bioavailability (both in vivo and in vitro) and efficacy varied with the type and variety from 2 mg/100 g to 8 mg/100 g. However, not all the efficacy studies indicated the iron levels in the millets. There were 30 research studies, including 22 human interventions and 8 in vitro studies, included in the meta-analysis which all discussed various outcomes such as hemoglobin level, serum ferritin level, and absorbed iron. The studies included finger millet, pearl millet, teff and sorghum, or a mixture of millets. The results of 19 studies conducted on anaemic individuals showed that there was a significant (p < 0.01) increase in hemoglobin levels by 13.2% following regular consumption (21 days to 4.5 years) of millets either as a meal or drink compared with regular diets where there was only 2.7% increase. Seven studies on adolescents showed increases in hemoglobin levels from 10.8 ± 1.4 (moderate anemia) to 12.2 ± 1.5 g/dl (normal). Two studies conducted on humans demonstrated that consumption of a pearl millet-based meal significantly increased the bioavailable iron (p < 0.01), with the percentage of bioavailability being 7.5 ± 1.6, and provided bioavailable iron of 1 ± 0.4 mg. Four studies conducted on humans showed significant increases in ferritin level (p < 0.05) up to 54.7%. Eight in-vitro studies showed that traditional processing methods such as fermentation and germination can improve bioavailable iron significantly (p < 0.01) by 3.4 and 2.2 times and contributed to 143 and 95% of the physiological requirement of women, respectively. Overall, this study showed that millets can reduce iron deficiency anemia.

scholarly journals Preparation and Characterization of a Novel Polysaccharide-Iron(III) Complex in Auricularia auricula Potentially Used as an Iron Supplement

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Tong Liu ◽  
Tingting Liu ◽  
Hongcheng Liu ◽  
Hongxiu Fan ◽  
Bingyu Chen ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Water Solubility ◽  
High Efficiency ◽  
Ph Value ◽  
Health Benefit ◽  
Iron Complex ◽  
Deficiency Anemia ◽  
Iron Supplement

Iron deficiency anemia has been a widespread disease. As an effective and stable iron supplement, the physiochemical properties of the polysaccharide iron complex have been widely studied. In this study, we characterized a novel polysaccharide-iron(III) complex extracted in an edible fungal species Auricularia auricular (AAPS-iron(III)). The highest iron content (28.40%) in the AAPS-iron(III) complex was obtained under the optimized preparation conditions including an AAPS to FeCl3∙6H2O ratio of 2:3 (w/w), a pH value of 8.0 in solution, a reaction temperature of 50°C, and a reaction time of 3 h. The physical and chemical properties of the AAPS-iron(III) complex were characterized by qualitative and quantitative analyses using scanning electron microscope, particle size distribution, thermogravimetric analyzer, Fourier transform infrared spectroscopy, circular dichroism, and 1H nuclear magnetic resonance. Result showed that, although the iron was bound to the polysaccharide, it was released under artificial gastrointestinal conditions. The AAPS-iron(III) complex exhibited high stability (under 50-256°C) and water solubility. The AAPS-iron(III) complex also showed high antioxidant activity in vitro, demonstrating an additional health benefit over other typical nonantioxidant iron nutritional supplements. Furthermore, the AAPS-iron(III) complex showed high efficiency on the treatment of the iron deficiency anemia in the model rats. Therefore, the AAPS-iron(III) complex can be used as a nutritional fortifier to supply iron in industrial processing and to assist the treatment of iron deficiency anemia.

Matriptase-2, A Novel Suppressor of Hepcidin.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. SCI-26-SCI-26
Author(s):  
Clara Camaschella ◽  
Antonella Nai ◽  
Alessia Pagani ◽  
Laura Silvestri
Keyword(s):  
Plasma Membrane ◽  
Iron Deficiency ◽  
Serine Protease ◽  
Iron Deficiency Anemia ◽  
Conflicts Of Interest ◽  
Bmp Signaling ◽  
Proteolytic Processing ◽  
Deficiency Anemia ◽  
Iron Release

Abstract Abstract SCI-26 Hepcidin inhibition is a process essential to increase iron release from duodenal cells and macrophages to plasma in conditions of high iron requests such as iron deficiency, hypoxia and erythropoietic expansion. Among the reported potential inhibitors of hepcidin the serine protease matriptase-2 has a strong effect in vivo both in mice and in humans. Matriptase-2 is a member of the transmembrane serine protease (TTPS) family, encoded by TMPRSS6 gene, whose expression is tissue restricted, mainly to the liver and to a lesser extent to kidney and olfactory epithelium. The role of matriptase-2 in iron metabolism was first defined in the Mask mouse, which after birth develops a type of iron deficiency anemia refractory to oral iron and a peculiar pattern of hair loss, because of inappropriate overexpression of hepcidin and impaired iron absorption, a phenotype due to the deletion of the matriptase-2 serine protease domain. An identical phenotype is reported in Tmprss6 -/- mice and a correspondent phenotype is observed in iron-refractory iron-deficiency anemia (IRIDA) patients. We have shown that matriptase-2 cleaves through a proteolytic processing the bone morphogenetic protein (BMP) co-receptor hemojuvelin from plasma membrane in vitro, indicating that the suppression of the BMP signaling is essential for hepcidin inhibition. The finding also suggests that the BMP pathway requiring hemojuvelin as coreceptor is the main iron-dependent pathway of hepcidin regulation. The hepcidin inhibitory effect is observed also in zebrafish and is abolished in the human homologue of Mask. We have investigated several missense TMPRSS6 mutants affecting different domains of the protein, reported in IRIDA patients, in comparison with wild type matriptase-2 and Mask. Transient overexpression in hepatoma cells shows that all mutants are deficient in the ability to inhibit the hepcidin promoter in a classic luciferase-based assay and that the decreased hepcidin inactivation broadly corresponds to the inability to cleave hemojuvelin from plasma membrane. Studies of the in vitro processing of the mutants indicate that determinants of the pathogenic effect others than the protease activity are the intracellular processing and the ability of self-activation of matriptase-2. These results have implications for the molecular pathogenesis of IRIDA. Disclosures No relevant conflicts of interest to declare.

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