scholarly journals Ankaferd Influences mRNA Expression of Iron-Regulated Genes During Iron-Deficiency Anemia

2017 ◽  
Vol 24 (6) ◽  
pp. 960-964 ◽  
Author(s):  
Afife Gulec ◽  
Sukru Gulec
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Messenger Rna ◽  
In Vitro Models ◽  
Ammonium Citrate ◽  
Deficiency Anemia ◽  
Ferric Ammonium Citrate ◽  
Marker Genes ◽  
Divalent Metal Transporter

Ankaferd Blood Stopper (ABS) comprises a mixture of plants and stops bleeding via forming a protein network by erythroid aggregation. Bleeding causes reduction of iron levels in body. It has been indicated that ABS contains significant amount of iron. Thus, we investigated the biological activity of ABS-derived iron on iron-regulated genes during iron-deficiency anemia (IDA). IDA We selected Caco-2 and HepG2 cell lines as in vitro models of human intestine and liver, respectively. Iron deficiency anemia was induced by deferoxamine. The cells were treated with ferric ammonium citrate (FAC) and ABS. Messenger RNA levels of iron-regulated genes were analyzed by quantitative reverse transcription polymerase chain reaction to elucidate whether iron in ABS behaved similar to inorganic iron (FAC) during IDA. The results showed that ABS-derived iron influenced transcriptions of iron-regulated marker genes, including divalent metal transporter ( Dmt1), transferrin receptor ( TfR), ankyrin repeat domain 37 ( Ankrd37), and hepcidin ( Hamp) in IDA-induced Caco-2 and HepG2 cells. Our results suggest that when ABS is used to stop tissue bleeding, it might have an ability to reduce levels of IDA.

In vitro cytokine production in patients with iron deficiency anemia

2004 ◽  
Vol 113 (3) ◽  
pp. 340-344 ◽  
Author(s):  
Michael Bergman ◽  
Hanna Bessler ◽  
Hertzel Salman ◽  
Dimitri Siomin ◽  
Rachel Straussberg ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Cytokine Production ◽  
Deficiency Anemia

scholarly journals Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Efrat Broide ◽  
Ram Reifen ◽  
Shay Matalon ◽  
Zipi Berkovich ◽  
Haim Shirin
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Iron Transport ◽  
Deficiency Anemia ◽  
Diabetic Patients ◽  
Divalent Metal Transporter 1 ◽  
Divalent Metal Transporter ◽  
Transporter Proteins ◽  
Group A ◽  
Group B

The role of iron transport proteins in the pathogenesis of anemia in patients with diabetes mellitus (T2DM) is still unclear. We investigated the expression of duodenal transporter proteins in diabetic patients with and without iron deficiency anemia (IDA). Methods. Overall, 39 patients were included: 16 with T2DM and IDA (group A), 11 with T2DM without IDA (group B), and 12 controls (group C). Duodenal mucosal expression of divalent metal transporter 1 (DMT1), ferroportin 1 (FPN), hephaestin (HEPH), and transferrin receptor 1 (TfR) was evaluated by Western blotting. Chronic disease activity markers were measured as well. Results. FPN expression was increased in group A compared to group B and controls: 1.17 (0.72–1.46), 0.76 (0.53–1.04), and 0.71 (0.64–0.86), respectively (p=0.011). TfR levels were over expressed in groups A and B compared to controls: 0.39 (0.26–0.61), 0.36 (0.24–0.43), and 0.18 (0.16–0.24), respectively, (p=0.004). The three groups did not differ significantly with regard to cellular HEPH and DMT1 expression. The normal CRP and serum ferritin levels, accompanied with normal FPN among diabetic patients without IDA, do not support the association of IDA with chronic inflammatory state. Conclusion. In patients with T2DM and IDA, duodenal iron transport protein expression might be dependent on body iron stores rather than by chronic inflammation or diabetes per se.

Iron Deficiency Anemia: Recovery from in vitro Oxidative Stress

1993 ◽  
Vol 90 (2) ◽  
pp. 94-98 ◽  
Author(s):  
M. Bartal ◽  
D. Mazor ◽  
A. Dvilansky ◽  
N. Meyerstein
Keyword(s):  
Oxidative Stress ◽  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Deficiency Anemia

scholarly journals MÖSSBAUER SPECTROSCOPY OF FERROUS FUMARATE IN PHARMACEUTICAL PRODUCT USED TO TREAT ANEMIA

2020 ◽  
Vol 21 (3) ◽  
pp. 43-49
Author(s):  
F.G. Vagizov ◽  
◽  
J. Nicolas Pineda M. ◽  
Keyword(s):  
Iron Deficiency ◽  
Isomer Shift ◽  
Debye Temperature ◽  
Quadrupole Splitting ◽  
Iron Deficiency Anemia ◽  
Public Health Problem ◽  
World Health ◽  
Deficiency Anemia ◽  
Mössbauer Measurements

The World Health Organization (WHO) considers iron deficiency anemia a serious public health problem in developing countries and recommends the use of iron tablets containing iron II for prevention and treatment. The results of Mössbauer measurements of the drug “Ferretab”, which is widely used in medicine for the treatment and prevention of iron deficiency anemia, are presented. This drug contains fumarate iron, C4H2FeO4, and has a small amount of folic acid. In this paper, the temperature dependence of isomer shift and quadrupole splitting values of 57Fe nuclei in iron fumarate were studied. The measurements show that when the temperature increases, the isomer shift and quadrupole splitting of 57Fe nuclei in iron fumarate decreases, the decrease in the isomer shift value is associated with the second-order Doppler effect. Based on Mössbauer measurements, the Debye temperature of this drug was determined. The Debye temperature gives us information about the strong bonding of 57Fe atoms with the environment. A high temperature value means a strong bond and vice versa, a small temperature value means a bond with low rigidity. The coupling constant (Debye temperature) defined for “Ferretab” iron nuclei has been compared with different Debye temperatures found in previous studies on some iron deficiency anemia drugs. Additionally, the values were compared with various clinical studies conducted in in-vivo and in-vitro for comparison of the efficacy of some of the most commonly used drugs to treat and prevent iron deficiency anemia. According to these comparisons, it was established a possible relationship between the Debye temperature of the iron atoms of the drugs under study and their effectiveness. It was noted that the lower the Debye temperature of iron atoms of the drug, the more iron absorbs the human body.

scholarly journals In Vitro Bioaccessibility and Bioavailability of Iron from Mature and Microgreen Fenugreek, Rocket and Broccoli

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1057
Author(s):  
Kholoud K. Khoja ◽  
Amy Buckley ◽  
Mohamad F. Aslam ◽  
Paul A. Sharp ◽  
Gladys O. Latunde-Dada
Keyword(s):  
Iron Deficiency ◽  
Iron Uptake ◽  
Mineral Content ◽  
Microwave Digestion ◽  
Surrogate Marker ◽  
Risk Groups ◽  
Ammonium Citrate ◽  
Iron Nutrition ◽  
Ferric Ammonium Citrate

Iron deficiency is a global epidemic affecting a third of the world’s population. Current efforts are focused on investigating sustainable ways to improve the bioavailability of iron in plant-based diets. Incorporating microgreens into the diet of at-risk groups in populations could be a useful tool in the management and prevention of iron deficiency. This study analysed and compared the mineral content and bioavailability of iron from microgreen and mature vegetables. The mineral content of rocket, broccoli and fenugreek microgreens and their mature counterparts was determined using microwave digestion and ICP-OES. Iron solubility and bioavailability from the vegetables were determined by a simulated gastrointestinal in vitro digestion and subsequent measurement of ferritin in Caco-2 cells as a surrogate marker of iron uptake. Iron contents of mature fenugreek and rocket were significantly higher than those of the microgreens. Mature fenugreek and broccoli showed significantly (p < 0.001) higher bioaccessibility and low-molecular-weight iron than found in the microgreens. Moreover, iron uptake by Caco-2 cells was significantly higher only from fenugreek microgreens than the mature vegetable. While all vegetables except broccoli enhanced FeSO4 uptake, the response to ferric ammonium citrate (FAC) was inhibitory apart from the mature rocket. Ascorbic acid significantly enhanced iron uptake from mature fenugreek and rocket. Microgreen fenugreek may be bred for a higher content of enhancers of iron availability as a strategy to improve iron nutrition in the populace.

The development of lentil derived protein–iron complexes and their effects on iron deficiency anemia in vitro

2020 ◽  
Vol 11 (5) ◽  
pp. 4185-4192 ◽  
Author(s):  
Ezgi Evcan ◽  
Sukru Gulec
Keyword(s):  
Iron Deficiency ◽  
Functional Properties ◽  
Iron Deficiency Anemia ◽  
Iron Complexes ◽  
Deficiency Anemia ◽  
Mrna Levels ◽  
Iron Minerals

Lentil derived proteins have the capacity to chelate iron minerals and hydrolysed protein–iron complexes have functional properties on iron deficiency anemia in in vitro by influencing mRNA levels of iron regulating genes.

scholarly journals In Vitro and In Vivo Evaluations of Mesoporous Iron Particles for Iron Bioavailability

2019 ◽  
Vol 20 (21) ◽  
pp. 5291
Author(s):  
Lin ◽  
Wu ◽  
Liao ◽  
Jakfar ◽  
Tang ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Cell Model ◽  
Deficiency Anemia ◽  
Iron Particles ◽  
Standard Drug ◽  
Iron Deficient ◽  
Commercial Iron

Chronic renal failure involving hemodialysis results in blood loss during filtration. Iron deficiency and iron deficiency anemia can result. A compensatory increase in iron dosage has many side effects including discomfort. Elemental iron is a highly-pure iron source, which reduces the frequency of dosages; the solubility decreases with increased particle size or pore size. In this study, synthesized mesoporous iron particles (MIPs) were used to relieve iron deficiency anemia. Their bioavailability was measured in vitro by a Caco-2 cell model and in vivo in iron-deficient rats. In vitro bioavailability of MIPs was examined by measuring ferritin content in the Caco-2 cell model. Iron uptake of MIPs was significantly higher than commercial iron particles, which were less porous. In vivo bioavailability of MIPs was examined by measuring body weight gain and red blood cell-related parameters, compared with the bioavailability of standard drug ferrous sulfate in iron-deficient anemic rats. Finally, average hemoglobin content and hemoglobin regeneration efficiency were significantly higher in anemic rats supplemented with commercial iron particles, compared to anemic controls. In the 28-day oral toxicity test, MIPs were not significantly toxic to rat physiology or tissue histopathology. Thus, MIPs may allow effective recovery of hemoglobin in iron deficiency anemia.

scholarly journals Comparative Study of Different Oral Iron Preparations in Females of Reproductive Age Group

2021 ◽  
pp. 93-100
Author(s):  
Megha Tiwari ◽  
Vishal Dubey ◽  
Nikita Srivastava
Keyword(s):  
Folic Acid ◽  
Iron Deficiency ◽  
Iron Hydroxide ◽  
Oral Iron ◽  
Reproductive Age ◽  
Ammonium Citrate ◽  
Deficiency Anemia ◽  
Ferric Ammonium Citrate ◽  
Significant Difference ◽  
Ferric Ammonium

Background: Anemia (anemia) is a decrease in the total amount of red blood cells (RBCs) or hemoglobin in the blood, or a lowered ability of the blood to carry oxygen. The commonness of iron inadequacy is high in all age bunch females. Around 32.4% of ladies have mellow iron deficiency, 14.19% ladies have direct sickliness, 2.2% have serious weakness. Objective: The aim of the study is to compare the efficacy and safety profile of oral iron formulations in iron deficiency anemia. Methods: A cross sectional study in patients with anemia receiving oral ferric ammonium citrate, folic acid, vitamin B12and iron hydroxide respectively was included. The patients were followed up once in 0 day, end of week, 1 month, for 3 months and observed for hematological improvement and adverse drug reactions (ADRs). Total 350 anemic patient were included in the study. Results: The data analyzed in 185 patients received ferric ammonium citrate, folic acid, 165 received, iron hydroxide,folic acid, vitamin B12 have significantly improved mean hemoglobin and anemia indices at the end of study, however, there was no significant differences between the groups when compared. “All four formulations showed similar ADR profile, there was no significant difference in adverse reactions.’’ Conclusion: Ferric ammonium citrate can be considered as best cost-effective choice for treatment of irons deficiency anemia.

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