Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

2020 ◽  
Vol 56 (68) ◽  
pp. 9894-9897
Author(s):  
Xuehe Lu ◽  
Xiaohui Wu ◽  
Tiantian Wu ◽  
Lin Han ◽  
Jianbing Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Delivery ◽  
Expression System ◽  
Eukaryotic Cells ◽  
Gene Expression System ◽  
Efficient Gene ◽  
Efficient Construction ◽  
Stable Linear

A TNA loop modified primer pair was designed to construct a stable terminal-closed linear gene, which can be used as an efficient gene expression system in eukaryotic cells.

scholarly journals A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c

2001 ◽  
Vol 65 (1) ◽  
pp. 185-189 ◽  
Author(s):  
Kiyoshi OZAWA ◽  
Fumiko YASUKAWA ◽  
Yumiko FUJIWARA ◽  
Hideo AKUTSU
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Cytochromes C

Flexible DNA junction assisted efficient construction of stable gene nanoparticles for gene delivery

2016 ◽  
Vol 52 (9) ◽  
pp. 1953-1956 ◽  
Author(s):  
Jianbing Liu ◽  
Yanyan Li ◽  
Dejun Ma ◽  
Di Ouyang ◽  
Zhen Xi
Keyword(s):  
Gene Delivery ◽  
Eukaryotic Cells ◽  
Stable Gene ◽  
Efficient Gene ◽  
Efficient Construction

A flexible DNA junction was designed to construct stable gene nanoparticles, which can be used as efficient gene cargo for eukaryotic cells.

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Artificial complementary chromatic acclimation gene expression system in Escherichia coli

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Dwi Ariyanti ◽  
Kazunori Ikebukuro ◽  
Koji Sode
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Light Exposure ◽  
Expression System ◽  
Red Light ◽  
Green Light ◽  
Light Illumination ◽  
Multiple Gene ◽  
Gene Expression System ◽  
Chromatic Acclimation

Abstract Background The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the PtrcΔLacO promoter. Results One G-box was inserted upstream of the cpcG2 promoter and a reporter gene, the rfp gene (green light-induced gene expression), and the other G-box was inserted between the PtrcΔLacO promoter and a reporter gene, the bfp gene (red light-induced gene expression). The Escherichia coli transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the rfp gene were observed, whereas the expression of the bfp gene was repressed. Under red light illumination, the transcription and translation of the bfp gene were observed, whereas the expression of the rfp gene was repressed. During the red and green light exposure cycles at every 6 h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed. Conclusion An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp. PCC6803, in E. coli. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.

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