Flexible DNA junction assisted efficient construction of stable gene nanoparticles for gene delivery

2016 ◽  
Vol 52 (9) ◽  
pp. 1953-1956 ◽  
Author(s):  
Jianbing Liu ◽  
Yanyan Li ◽  
Dejun Ma ◽  
Di Ouyang ◽  
Zhen Xi
Keyword(s):  
Gene Delivery ◽  
Eukaryotic Cells ◽  
Stable Gene ◽  
Efficient Gene ◽  
Efficient Construction

A flexible DNA junction was designed to construct stable gene nanoparticles, which can be used as efficient gene cargo for eukaryotic cells.

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

2020 ◽  
Vol 56 (68) ◽  
pp. 9894-9897
Author(s):  
Xuehe Lu ◽  
Xiaohui Wu ◽  
Tiantian Wu ◽  
Lin Han ◽  
Jianbing Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Delivery ◽  
Expression System ◽  
Eukaryotic Cells ◽  
Gene Expression System ◽  
Efficient Gene ◽  
Efficient Construction ◽  
Stable Linear

A TNA loop modified primer pair was designed to construct a stable terminal-closed linear gene, which can be used as an efficient gene expression system in eukaryotic cells.

Efficient construction of stable gene nanoparticles through polymerase chain reaction with flexible branched primers for gene delivery

2015 ◽  
Vol 51 (44) ◽  
pp. 9208-9211 ◽  
Author(s):  
Jianbing Liu ◽  
Runyu Wang ◽  
Dejun Ma ◽  
Di Ouyang ◽  
Zhen Xi
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Delivery ◽  
Stable Gene ◽  
Chain Reaction ◽  
Efficient Construction ◽  
Polymerase Chain

Flexible branched primers were designed to construct stable gene nanoparticles through polymerase chain reaction for gene delivery.

Genetic Engineering of AAV Capsid Gene for Gene Therapy Application

2020 ◽  
Vol 20 (5) ◽  
pp. 321-332
Author(s):  
Yunbo Liu ◽  
Xu Zhang ◽  
Lin Yang
Keyword(s):  
Gene Therapy ◽  
Neutralizing Antibodies ◽  
Human Subjects ◽  
Genetic Diseases ◽  
Capsid Gene ◽  
Human Populations ◽  
Stable Gene ◽  
Efficient Gene ◽  
Gene Therapy Application

Adeno-associated virus (AAV) is a promising vector for in vivo gene therapy because of its excellent safety profile and ability to mediate stable gene expression in human subjects. However, there are still numerous challenges that need to be resolved before this gene delivery vehicle is used in clinical applications, such as the inability of AAV to effectively target specific tissues, preexisting neutralizing antibodies in human populations, and a limited AAV packaging capacity. Over the past two decades, much genetic modification work has been performed with the AAV capsid gene, resulting in a large number of variants with modified characteristics, rendering AAV a versatile vector for more efficient gene therapy applications for different genetic diseases.

Amino acids functionalized dendrimers with nucleus accumulation for efficient gene delivery

2021 ◽  
pp. 120641
Author(s):  
Guoxin Tan ◽  
Jiayang Li ◽  
Dandan Liu ◽  
Hao Pan ◽  
Renfang Zhu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Gene Delivery ◽  
Efficient Gene

scholarly journals Carbon Dot Nanoparticles: Exploring the Potential Use for Gene Delivery in Ophthalmic Diseases

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 935
Author(s):  
Manas R. Biswal ◽  
Sofia Bhatia
Keyword(s):  
Gene Delivery ◽  
Therapeutic Option ◽  
Retinal Dystrophy ◽  
Retinal Cells ◽  
Adeno Associated Virus ◽  
Inherited Retinal Dystrophies ◽  
Retinal Dystrophies ◽  
Efficient Gene ◽  
New Type ◽  
Viral Nanoparticles

Ocular gene therapy offers significant potential for preventing retinal dystrophy in patients with inherited retinal dystrophies (IRD). Adeno-associated virus (AAV) based gene transfer is the most common and successful gene delivery approach to the eye. These days, many studies are using non-viral nanoparticles (NPs) as an alternative therapeutic option because of their unique properties and biocompatibility. Here, we discuss the potential of carbon dots (CDs), a new type of nanocarrier for gene delivery to the retinal cells. The unique physicochemical properties of CDs (such as optical, electronic, and catalytic) make them suitable for biosensing, imaging, drug, and gene delivery applications. Efficient gene delivery to the retinal cells using CDs depends on various factors, such as photoluminescence, quantum yield, biocompatibility, size, and shape. In this review, we focused on different approaches used to synthesize CDs, classify CDs, various pathways for the intake of gene-loaded carbon nanoparticles inside the cell, and multiple studies that worked on transferring nucleic acid in the eye using CDs.

Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector

2015 ◽  
Vol 23 (8) ◽  
pp. 726-733 ◽  
Author(s):  
Nan Young Ahn ◽  
Tae-Hun Kim ◽  
Su Jeong Song ◽  
Jeong-Mi Moon ◽  
Tai Hwan Ha ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
Efficient Gene

scholarly journals Efficient gene delivery and silencing of mouse and human pancreatic islets

2010 ◽  
Vol 10 (1) ◽  
pp. 28 ◽  
Author(s):  
Bruno Lefebvre ◽  
Brigitte Vandewalle ◽  
Justine Longue ◽  
Ericka Moerman ◽  
Bruno Lukowiak ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Efficient Gene

Efficient gene delivery using chitosan–polyethylenimine hybrid systems

2008 ◽  
Vol 3 (2) ◽  
pp. 025013 ◽  
Author(s):  
Hu-Lin Jiang ◽  
Tae-Hee Kim ◽  
You-Kyoung Kim ◽  
In-Young Park ◽  
Myung-Haing Cho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hybrid Systems ◽  
Efficient Gene
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