Controlled nonviral gene delivery and expression using stable neural stem cell line transfected with a hypoxia-inducible gene expression system

2010 ◽  
Vol 12 (12) ◽  
pp. 990-1001 ◽  
Author(s):  
Meng-Lu Liu ◽  
Jin Soo Oh ◽  
Sung Su An ◽  
William A. Pennant ◽  
Hyo Jin Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Gene Delivery ◽  
Cell Line ◽  
Expression System ◽  
Nonviral Gene Delivery ◽  
Stem Cell Line ◽  
Gene Expression System ◽  
Neural Stem Cell Line ◽  
Inducible Gene

A Novel, Immortal, and Multipotent Human Neural Stem Cell Line Generating Functional Neurons and Oligodendrocytes

Stem Cells ◽  
2007 ◽  
Vol 25 (9) ◽  
pp. 2312-2321 ◽  
Author(s):  
Lidia De Filippis ◽  
Giuseppe Lamorte ◽  
Evan Y. Snyder ◽  
Antonio Malgaroli ◽  
Angelo L. Vescovi
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Neural Stem Cell ◽  
Stem Cell Line ◽  
Generating Functional ◽  
Human Neural Stem Cell ◽  
Neural Stem Cell Line

scholarly journals Functional properties of the human ventral mesencephalic neural stem cell line hVM1

2010 ◽  
Vol 223 (2) ◽  
pp. 653-656 ◽  
Author(s):  
Jan Tønnesen ◽  
Emma Gonzalez Seiz ◽  
Milagros Ramos ◽  
Olle Lindvall ◽  
Alberto Martinez-Serrano ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Neural Stem Cell ◽  
Functional Properties ◽  
Stem Cell Line ◽  
Neural Stem Cell Line ◽  
Ventral Mesencephalic

scholarly journals Cumate-Inducible Gene Expression System for Sphingomonads and Other Alphaproteobacteria

2013 ◽  
Vol 79 (21) ◽  
pp. 6795-6802 ◽  
Author(s):  
Andreas Kaczmarczyk ◽  
Julia A. Vorholt ◽  
Anne Francez-Charlot
Keyword(s):  
Gene Expression ◽  
Caulobacter Crescentus ◽  
Paracoccus Denitrificans ◽  
Expression System ◽  
Model Organisms ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Content Type ◽  
Wide Range ◽  
Inducible Gene

ABSTRACTTunable promoters represent a pivotal genetic tool for a wide range of applications. Here we present such a system for sphingomonads, a phylogenetically diverse group of bacteria that have gained much interest for their potential in bioremediation and their use in industry and for which no dedicated inducible gene expression system has been described so far. A strong, constitutive synthetic promoter was first identified through a genetic screen and subsequently combined with the repressor and the operator sites of thePseudomonas putidaF1cym/cmtsystem. The resulting promoter, termed PQ5, responds rapidly to the inducer cumate and shows a maximal induction ratio of 2 to 3 orders of magnitude in the different sphingomonads tested. Moreover, it was also functional in otherAlphaproteobacteria, such as the model organismsCaulobacter crescentus,Paracoccus denitrificans, andMethylobacterium extorquens. In the noninduced state, expression from PQ5is low enough to allow gene depletion analysis, as demonstrated with the essential genephyPofSphingomonassp. strain Fr1. A set of PQ5-based plasmids has been constructed allowing fusions to affinity tags or fluorescent proteins.

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