Grafting multi-maleimides on antisense oligonucleotide to enhance its cellular uptake and gene silencing capability

2020 ◽  
Vol 56 (54) ◽  
pp. 7439-7442
Author(s):  
Yuanyuan Guo ◽  
Jiao Zhang ◽  
Gaifang Pan ◽  
Chung Hang Jonathan Choi ◽  
Ping Wang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Gene Silencing ◽  
Cellular Uptake ◽  
Antisense Oligonucleotide ◽  
Gene Knockdown ◽  
Cell Internalization

Grafting multi-maleimides on antisense oligonucleotide enables its click conjugation to membrane proteins, inducing promoted non-endocytic cell internalization and gene knockdown.

Enhanced cellular uptake and gene silencing activity of siRNA molecules mediated by chitosan-derivative nanocomplexes

2014 ◽  
Vol 473 (1-2) ◽  
pp. 579-590 ◽  
Author(s):  
Diana Guzman-Villanueva ◽  
Ibrahim M. El-Sherbiny ◽  
Alexander V. Vlassov ◽  
Dea Herrera-Ruiz ◽  
Hugh D.C. Smyth
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Chitosan Derivative

Quaternized starch-based carrier for siRNA delivery: From cellular uptake to gene silencing

2014 ◽  
Vol 185 ◽  
pp. 109-120 ◽  
Author(s):  
Eliz Amar-Lewis ◽  
Aharon Azagury ◽  
Ramesh Chintakunta ◽  
Riki Goldbart ◽  
Tamar Traitel ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Sirna Delivery

scholarly journals Enhanced biostability and cellular uptake of zinc oxide nanocrystals shielded with a phospholipid bilayer

2017 ◽  
Vol 5 (44) ◽  
pp. 8799-8813 ◽  
Author(s):  
B. Dumontel ◽  
M. Canta ◽  
H. Engelke ◽  
A. Chiodoni ◽  
L. Racca ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Surface Chemistry ◽  
Lipid Bilayer ◽  
Cellular Uptake ◽  
Biological Fluids ◽  
Phospholipid Bilayer ◽  
Cell Internalization ◽  
Zno Nanocrystals ◽  
Bilayer Assembly

The surface chemistry and charge of zinc oxide nanocrystals influence their behaviour in biological fluids. A novel lipid bilayer assembly is developed to shield ZnO nanocrystals improving their stability and cell internalization.

scholarly journals Cell-Penetrating Peptides: a Useful Tool for the Delivery of Various Cargoes Into Cells

2018 ◽  
pp. S267-S279 ◽  
Author(s):  
E. BÖHMOVÁ ◽  
D. MACHOVÁ ◽  
M. PECHAR ◽  
R. POLA ◽  
K. VENCLÍKOVÁ ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Cellular Membrane ◽  
Cell Penetrating Peptides ◽  
Cell Entry ◽  
Biologically Active ◽  
Cell Internalization ◽  
Structural Types ◽  
Cell Penetrating ◽  
History Of ◽  
Preclinical And Clinical Studies

Cell-penetrating compounds are substances that enhance the cellular uptake of various molecular cargoes that do not easily cross the cellular membrane. The majority of cell-penetrating compounds described in the literature are cell-penetrating peptides (CPPs). This review summarizes the various structural types of cell-penetrating compounds, with the main focus on CPPs. The authors present a brief overview of the history of CPPs, discuss the various types of conjugation of CPPs to biologically active cargoes intended for cell internalization, examine the cell-entry mechanisms of CPPs, and report on the applications of CPPs in research and in preclinical and clinical studies.

Stability and cellular uptake of polymerized siRNA (poly-siRNA)/polyethylenimine (PEI) complexes for efficient gene silencing

2010 ◽  
Vol 141 (3) ◽  
pp. 339-346 ◽  
Author(s):  
Seung-Young Lee ◽  
Myung Sook Huh ◽  
Seulki Lee ◽  
So Jin Lee ◽  
Hyunjin Chung ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Efficient Gene

scholarly journals A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells

2015 ◽  
Vol 43 (19) ◽  
pp. 9350-9361 ◽  
Author(s):  
Daniela Castanotto ◽  
Min Lin ◽  
Claudia Kowolik ◽  
LiAnn Wang ◽  
Xiao-Qin Ren ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Antisense Oligonucleotide ◽  
Mammalian Cells

scholarly journals Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution

2010 ◽  
Vol 24 (3-4) ◽  
pp. 303-307 ◽  
Author(s):  
Petr Praus ◽  
Eva Kocišová ◽  
Peter Mojzeš ◽  
Josef Štepánek ◽  
Franck Sureau ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Decay Time ◽  
Spectral Region ◽  
High Frequency ◽  
Antisense Oligonucleotide ◽  
Drug Distribution ◽  
Image Intensifier ◽  
Living Cells ◽  
Time Data ◽  
Time Resolved

Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

Sign in / Sign up

Export Citation Format

Share Document