Di- and Triblock siRNA-PEG Copolymers: PEG Density Effect of Polyelectrolyte Complexes on Cellular Uptake and Gene Silencing Efficiency

2010 ◽  
Vol 11 (3) ◽  
pp. 410-418 ◽  
Author(s):  
Soo Hyeon Lee ◽  
Hyejung Mok ◽  
Tae Gwan Park
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Density Effect ◽  
Polyelectrolyte Complexes

Enhanced cellular uptake and gene silencing activity of siRNA molecules mediated by chitosan-derivative nanocomplexes

2014 ◽  
Vol 473 (1-2) ◽  
pp. 579-590 ◽  
Author(s):  
Diana Guzman-Villanueva ◽  
Ibrahim M. El-Sherbiny ◽  
Alexander V. Vlassov ◽  
Dea Herrera-Ruiz ◽  
Hugh D.C. Smyth
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Chitosan Derivative

Quaternized starch-based carrier for siRNA delivery: From cellular uptake to gene silencing

2014 ◽  
Vol 185 ◽  
pp. 109-120 ◽  
Author(s):  
Eliz Amar-Lewis ◽  
Aharon Azagury ◽  
Ramesh Chintakunta ◽  
Riki Goldbart ◽  
Tamar Traitel ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Sirna Delivery

Stability and cellular uptake of polymerized siRNA (poly-siRNA)/polyethylenimine (PEI) complexes for efficient gene silencing

2010 ◽  
Vol 141 (3) ◽  
pp. 339-346 ◽  
Author(s):  
Seung-Young Lee ◽  
Myung Sook Huh ◽  
Seulki Lee ◽  
So Jin Lee ◽  
Hyunjin Chung ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Efficient Gene

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

Niosomes Containing Spermine-Based Cationic Lipid with Different Linkers for siRNA Delivery

2019 ◽  
Vol 819 ◽  
pp. 169-174
Author(s):  
Supusson Pengnam ◽  
Praneet Opanasopit ◽  
Theerasak Rojanarata ◽  
Nattisa Ni-yomtham ◽  
Boon Ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Sirna Delivery ◽  
Weight Ratio ◽  
Cationic Lipid ◽  
Cationic Lipids ◽  
Molar Ratio ◽  
Ionic Surfactant ◽  
Low Toxicity ◽  
Cationic Niosomes

Niosomes are a lipid nanoparticle which have been widely used as non-viral carrier for therapeutic DNA or siRNA. They are formulated from non-ionic surfactant and other helper lipids. The aim of this study were to formulate niosome containing spermine-based cationic lipid with different linkers and to evaluate the efficiency of siRNA delivery in cervical cancer cell (HeLa cell). The niosomes were formulated from cholesterol (Chol), Span 20 and different cationic lipid (Ay, By, Cy and Dy) at various molar ratios. The properties of niosomes and ability of niosome to complex with siRNA were characterized. The cellular uptake, gene silencing efficiency and cytotoxicity were also determined. From the results, niosomes formulated at Chol:Span20:lipid molar ratio of 2.5:2.5:2 showed positive zeta potential and they were in nanosize (<200 nm). The binding ability of cationic niosomes to siRNA depended on types of cationic lipid. Among niosome/siRNA complexes, the niosome By/siRNA complex provided the highest gene silencing efficiency at weight ratio of 20. The highest cellular uptake also obtained by using niosome By as a carrier. The cytotoxicity revealed that cationic niosomes had low toxicity (cell viability > 80%). In conclusion, the cationic niosomes prepared from Chol, Span 20 and spermine-based cationic lipids are able to complex with siRNA and suitable for siRNA delivery with low toxicity.

scholarly journals Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group

2011 ◽  
Vol 40 (5) ◽  
pp. 2330-2344 ◽  
Author(s):  
Natalya S. Petrova ◽  
Ivan V. Chernikov ◽  
Mariya I. Meschaninova ◽  
IIya S. Dovydenko ◽  
Aliya G. Venyaminova ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Carrier Free

scholarly journals Lipid-modified oligonucleotide conjugates: Insights into gene silencing, interaction with model membranes and cellular uptake mechanisms

2017 ◽  
Vol 25 (1) ◽  
pp. 175-186 ◽  
Author(s):  
Begoña Ugarte-Uribe ◽  
Santiago Grijalvo ◽  
Samuel Núñez Pertíñez ◽  
Jon V. Busto ◽  
César Martín ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Model Membranes ◽  
Oligonucleotide Conjugates ◽  
Modified Oligonucleotide ◽  
Uptake Mechanisms

scholarly journals Disulfide-Based Poly(amido amine)s for siRNA Delivery: Effects of Structure on siRNA Complexation, Cellular Uptake, Gene Silencing and Toxicity

2010 ◽  
Vol 28 (5) ◽  
pp. 1013-1022 ◽  
Author(s):  
Pieter Vader ◽  
Leonardus J. van der Aa ◽  
Johan F. J. Engbersen ◽  
Gert Storm ◽  
Raymond M. Schiffelers
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Sirna Delivery

scholarly journals Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing

2019 ◽  
Vol 141 ◽  
pp. 37-50 ◽  
Author(s):  
Cornelia Lorenzer ◽  
Sonja Streußnig ◽  
Emilia Tot ◽  
Anna-Maria Winkler ◽  
Hannes Merten ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Targeted Delivery

Surface coating of siRNA–peptidomimetic nano-self-assemblies with anionic lipid bilayers: enhanced gene silencing and reduced adverse effects in vitro

Nanoscale ◽  
2015 ◽  
Vol 7 (46) ◽  
pp. 19687-19698 ◽  
Author(s):  
Xianghui Zeng ◽  
Anne Marit de Groot ◽  
Alice J. A. M. Sijts ◽  
Femke Broere ◽  
Erik Oude Blenke ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Gene Silencing ◽  
Lipid Bilayers ◽  
Cellular Uptake ◽  
Surface Coating ◽  
Endosomal Escape ◽  
Anionic Lipid

Improved cellular uptake and endosomal escape of siRNA via surface coating of siRNA–peptidomimetic nanocomplexes with anionic lipid bilayers.

Sign in / Sign up

Export Citation Format

Share Document