Three-dimensional DNA nanostructures for dual-color microRNA imaging in living cells via hybridization chain reaction

2020 ◽  
Vol 56 (49) ◽  
pp. 6668-6671
Author(s):  
Meng-Mei Lv ◽  
Zhan Wu ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Nanostructure ◽  
Dual Color ◽  
Dna Tetrahedron

A well-defined 3D DNA nanostructure was developed by combination of DNA tetrahedron and Y-shaped DNA, which allowed multiplexed, signal amplified fluorescent imaging of miRNAs in living cells via hybridization chain reaction.

scholarly journals Three-dimensional DNA nanostructures to improve the hyperbranched hybridization chain reaction

2019 ◽  
Vol 10 (42) ◽  
pp. 9758-9767 ◽  
Author(s):  
Jing Wang ◽  
Dong-Xia Wang ◽  
Jia-Yi Ma ◽  
Ya-Xin Wang ◽  
De-Ming Kong
Keyword(s):  
Nucleic Acid ◽  
Three Dimensional ◽  
Nucleic Acid Amplification ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Nucleic Acid Amplification Techniques ◽  
Detection Of Biomarkers

Nonenzymatic nucleic acid amplification techniques (e.g. the hybridization chain reaction, HCR) have shown promising potential for amplified detection of biomarkers.

Ratiometric Fluorescent DNA Nanostructure for Mitochondrial ATP Imaging in Living Cells Based on Hybridization Chain Reaction

2021 ◽  
Vol 93 (17) ◽  
pp. 6715-6722
Author(s):  
Lei Luo ◽  
Min Wang ◽  
Yuan Zhou ◽  
Dongliu Xiang ◽  
Qing Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Dna Nanostructure

Ultrasensitive ratiometric detection of Pb2+ using DNA tetrahedron-mediated hyperbranched hybridization chain reaction

2021 ◽  
Vol 1147 ◽  
pp. 170-177
Author(s):  
Pingping Ji ◽  
Guimei Han ◽  
Yan Huang ◽  
Hongxin Jiang ◽  
Qiwen Zhou ◽  
...  
Keyword(s):  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Ratiometric Detection ◽  
Dna Tetrahedron

scholarly journals Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Feng Li ◽  
Wenting Yu ◽  
Jiaojiao Zhang ◽  
Yuhang Dong ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Steric Effect ◽  
Sirna Delivery ◽  
Dna Nanotechnology ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Hairpins ◽  
In Vivo Experiments ◽  
Dna And Rna

AbstractDNA nanostructures have been demonstrated as promising carriers for gene delivery. In the carrier design, spatiotemporally programmable assembly of DNA under nanoconfinement is important but has proven highly challenging due to the complexity–scalability–error of DNA. Herein, a DNA nanotechnology-based strategy via the cascade hybridization chain reaction (HCR) of DNA hairpins in polymeric nanoframework has been developed to achieve spatiotemporally programmable assembly of DNA under nanoconfinement for precise siRNA delivery. The nanoframework is prepared via precipitation polymerization with Acrydite-DNA as cross-linker. The potential energy stored in the loops of DNA hairpins can overcome the steric effect in the nanoframework, which can help initiate cascade HCR of DNA hairpins and achieve efficient siRNA loading. The designer tethering sequence between DNA and RNA guarantees a triphosadenine triggered siRNA release specifically in cellular cytoplasm. Nanoframework provides stability and ease of functionalization, which helps address the complexity–scalability–error of DNA. It is exemplified that the phenylboronate installation on nanoframework enhanced cellular uptake and smoothed the lysosomal escape. Cellular results show that the siRNA loaded nanoframework down-regulated the levels of relevant mRNA and protein. In vivo experiments show significant therapeutic efficacy of using siPLK1 loaded nanoframework to suppress tumor growth.

Imaging of Receptor Dimers in Zebrafish and Living Cells via Aptamer Recognition and Proximity-Induced Hybridization Chain Reaction

2018 ◽  
Vol 90 (24) ◽  
pp. 14433-14438 ◽  
Author(s):  
Liping Wang ◽  
Wei Li ◽  
Jin Sun ◽  
Su-Yun Zhang ◽  
Sheng Yang ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Receptor Dimers

A novel FRET-based dendritic hybridization chain reaction for tumour-related mRNA imaging

2022 ◽  
Author(s):  
Shiyuan Liu ◽  
Zhenxiang Liu ◽  
Huimin Wang ◽  
Jiaoli Wang ◽  
Ruiting Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A novel FRET-based dendritic hybridization chain reaction (D-HCR) for TK1 mRNA imaging was developed in living cells. Compared to the traditional complex D-HCR methods, it has the following advantages, including...

Intracellular Nonenzymatic In Situ Growth of Three-Dimensional DNA Nanostructures for Imaging Specific Biomolecules in Living Cells

ACS Nano ◽  
2020 ◽  
Vol 14 (8) ◽  
pp. 9572-9584
Author(s):  
Ran Liu ◽  
Songbai Zhang ◽  
Ting-Ting Zheng ◽  
Yan-Ru Chen ◽  
Jing-Ting Wu ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
In Situ Growth ◽  
Dna Nanostructures

scholarly journals Branched Hybridization Chain Reaction Circuit for Ultrasensitive Localizable Imaging of mRNA in Living Cells

2018 ◽  
Vol 90 (3) ◽  
pp. 1502-1505 ◽  
Author(s):  
Lan Liu ◽  
Jin-Wen Liu ◽  
Han Wu ◽  
Xiang-Nan Wang ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction
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