A novel FRET-based dendritic hybridization chain reaction for tumour-related mRNA imaging

2022 ◽  
Author(s):  
Shiyuan Liu ◽  
Zhenxiang Liu ◽  
Huimin Wang ◽  
Jiaoli Wang ◽  
Ruiting Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A novel FRET-based dendritic hybridization chain reaction (D-HCR) for TK1 mRNA imaging was developed in living cells. Compared to the traditional complex D-HCR methods, it has the following advantages, including...

Imaging of Receptor Dimers in Zebrafish and Living Cells via Aptamer Recognition and Proximity-Induced Hybridization Chain Reaction

2018 ◽  
Vol 90 (24) ◽  
pp. 14433-14438 ◽  
Author(s):  
Liping Wang ◽  
Wei Li ◽  
Jin Sun ◽  
Su-Yun Zhang ◽  
Sheng Yang ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Receptor Dimers

scholarly journals Branched Hybridization Chain Reaction Circuit for Ultrasensitive Localizable Imaging of mRNA in Living Cells

2018 ◽  
Vol 90 (3) ◽  
pp. 1502-1505 ◽  
Author(s):  
Lan Liu ◽  
Jin-Wen Liu ◽  
Han Wu ◽  
Xiang-Nan Wang ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

scholarly journals A proteinase-free DNA replication machinery for in vitro and in vivo amplified MicroRNA imaging

2020 ◽  
Vol 48 (10) ◽  
pp. e60-e60 ◽  
Author(s):  
Jie Wei ◽  
Huimin Wang ◽  
Xue Gong ◽  
Qing Wang ◽  
Hong Wang ◽  
...  
Keyword(s):  
Dna Replication ◽  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Replication Machinery ◽  
Chain Reaction ◽  
Free Dna ◽  
Sensing Platform ◽  
Dna Machine

Abstract The construction of robust, modular and compact DNA machinery facilitates us to build more intelligent and ingenious sensing strategies in complex biological systems. However, the performance of conventional DNA amplifiers is always impeded by their limited in-depth amplifications and miscellaneously enzymatic requirements. Here, a proteinase-free reciprocal DNA replication machinery is developed by exploiting the synergistic cross-activation between hybridization chain reaction (HCR) and DNAzyme. The DNAzyme provides an efficient way to simplify the sophisticated design of HCR machinery and simultaneously to promote the amplification capacity. And the HCR-assembled tandem DNAzyme nanowires produce numerous new triggers for reversely stimulating HCR amplifier as systematically explored by experiments and computer-aided simulations. The reciprocal amplifier can be executed as a versatile and powerful sensing platform for analyzing miRNA in living cells and even in mice, originating from the inherent reaction accelerations and multiple-guaranteed recognitions. The reciprocal catalytic DNA machine holds great potential in clinical diagnosis and assessment.

Three-dimensional DNA nanostructures for dual-color microRNA imaging in living cells via hybridization chain reaction

2020 ◽  
Vol 56 (49) ◽  
pp. 6668-6671
Author(s):  
Meng-Mei Lv ◽  
Zhan Wu ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Nanostructure ◽  
Dual Color ◽  
Dna Tetrahedron

A well-defined 3D DNA nanostructure was developed by combination of DNA tetrahedron and Y-shaped DNA, which allowed multiplexed, signal amplified fluorescent imaging of miRNAs in living cells via hybridization chain reaction.

Biodegradable MnO2 nanosheet mediated hybridization chain reaction for imaging of human apurinic/apyrimidinic endonuclease 1 activity in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (43) ◽  
pp. 20456-20460 ◽  
Author(s):  
Guangli Li ◽  
Junjie Li ◽  
Qing Li
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Amplification Assay ◽  
Mno2 Nanosheet

A highly sensitive enzyme-free amplification assay for the detection of apurinic/apyrimidinic endonuclease 1 activity was developed.

Ratiometric Fluorescent DNA Nanostructure for Mitochondrial ATP Imaging in Living Cells Based on Hybridization Chain Reaction

2021 ◽  
Vol 93 (17) ◽  
pp. 6715-6722
Author(s):  
Lei Luo ◽  
Min Wang ◽  
Yuan Zhou ◽  
Dongliu Xiang ◽  
Qing Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Dna Nanostructure
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