Intracellular Nonenzymatic In Situ Growth of Three-Dimensional DNA Nanostructures for Imaging Specific Biomolecules in Living Cells

ACS Nano ◽  
2020 ◽  
Vol 14 (8) ◽  
pp. 9572-9584
Author(s):  
Ran Liu ◽  
Songbai Zhang ◽  
Ting-Ting Zheng ◽  
Yan-Ru Chen ◽  
Jing-Ting Wu ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
In Situ Growth ◽  
Dna Nanostructures

scholarly journals Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.

1993 ◽  
Vol 123 (2) ◽  
pp. 431-441 ◽  
Author(s):  
K Ainger ◽  
D Avossa ◽  
F Morgan ◽  
S J Hill ◽  
C Barry ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Branch Points ◽  
Rna Granules ◽  
Intracellular Movement ◽  
Directional Movement ◽  
Ionic Detergent ◽  
Mrna Granules

We have studied transport and localization of MBP mRNA in oligodendrocytes in culture by microinjecting labeled mRNA into living cells and analyzing the intracellular distribution of the injected RNA by confocal microscopy. Injected mRNA initially appears dispersed in the perikaryon. Within minutes, the RNA forms granules which, in the case of MBP mRNA, are transported down the processes to the periphery of the cell where the distribution again becomes dispersed. In situ hybridization shows that endogenous MBP mRNA in oligodendrocytes also appears as granules in the perikaryon and processes and dispersed in the peripheral membranes. The granules are not released by extraction with non-ionic detergent, indicating that they are associated with the cytoskeletal matrix. Three dimensional visualization indicates that MBP mRNA granules are often aligned in tracks along microtubules traversing the cytoplasm and processes. Several distinct patterns of granule movement are observed. Granules in the processes undergo sustained directional movement with a velocity of approximately 0.2 micron/s. Granules at branch points undergo oscillatory motion with a mean displacement of 0.1 micron/s. Granules in the periphery of the cell circulate randomly with a mean displacement of approximately 1 micron/s. The results are discussed in terms of a multi-step pathway for transport and localization of MBP mRNA in oligodendrocytes. This work represents the first characterization of intracellular movement of mRNA in living cells, and the first description of the role of RNA granules in transport and localization of mRNA in cells.

Three-dimensional DNA nanostructures for dual-color microRNA imaging in living cells via hybridization chain reaction

2020 ◽  
Vol 56 (49) ◽  
pp. 6668-6671
Author(s):  
Meng-Mei Lv ◽  
Zhan Wu ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Nanostructure ◽  
Dual Color ◽  
Dna Tetrahedron

A well-defined 3D DNA nanostructure was developed by combination of DNA tetrahedron and Y-shaped DNA, which allowed multiplexed, signal amplified fluorescent imaging of miRNAs in living cells via hybridization chain reaction.

In Situ Growth of Three-Dimensional Graphene Films for Signal-On Electrochemical Biosensing of Various Analytes

2016 ◽  
Vol 88 (21) ◽  
pp. 10667-10674 ◽  
Author(s):  
Dongqing Kong ◽  
Sai Bi ◽  
Zonghua Wang ◽  
Jianfei Xia ◽  
Feifei Zhang
Keyword(s):  
Three Dimensional ◽  
In Situ Growth ◽  
Electrochemical Biosensing ◽  
Graphene Films

In situ growth gold nanoparticles in three-dimensional sugarcane membrane for flow catalytical and antibacterial application

2021 ◽  
Vol 402 ◽  
pp. 123445
Author(s):  
Qingtong Zhang ◽  
Mingfu Li ◽  
Bin Luo ◽  
Yuying Luo ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Three Dimensional ◽  
In Situ Growth
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