scholarly journals An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

2021 ◽  
Author(s):  
Regina A. Baglia ◽  
Kira R. Mills ◽  
Koushambi Mitra ◽  
Jasmine N. Tutol ◽  
Darby Ball ◽  
...  
Keyword(s):  
Computational Modeling ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Biological Application ◽  
Phenol Sulfotransferase

Here, we report the development, computational modeling, in vitro enzymology, and biological application of an activity-based fluorescent sensor for the human phenol sulfotransferase SULT1A1.

scholarly journals An Activity-Based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells

2020 ◽  
Author(s):  
Regina A. Baglia ◽  
Kira Mills ◽  
Koushambi Mitra ◽  
Jasmine Tutol ◽  
Darby Ball ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Biological Application ◽  
Intact Cells ◽  
Phenol Sulfotransferase ◽  
Endogenous Metabolites ◽  
Direct Readout ◽  
Inorganic Sulfate

<p>The biological activation and incorporation of inorganic sulfate proceeds via a process known as sulfurylation. Transfer of a sulfuryl moiety from the activated sulfate donor, 3’-phosphoadenosine-5’-phosphosulfate (PAPS), to hydroxy-containing substrates by human phenol sulfotransferases (SULT1 family) alters substrate solubility and charge to affect the metabolism of endogenous metabolites, xenobiotics, and drugs. Current methods to monitor SULT1 activity in living cells primarily rely on radiolabeling and/or cell extractions, but these methods do not provide a direct readout of enzyme activity with a dynamic, temporally resolved spatial map in live, intact cells. To fill this gap, here, we present the development, computational modeling, <i>in vitro</i> enzymology, and biological application of Sulfotransferase Sensor-3, STS-3, an activity-based fluorescent sensor for SULT1A1, the most widely expressed and promiscuous SULT1 isoform. </p>

scholarly journals An Activity-Based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells

2020 ◽  
Author(s):  
Regina A. Baglia ◽  
Kira Mills ◽  
Koushambi Mitra ◽  
Jasmine Tutol ◽  
Darby Ball ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Biological Application ◽  
Intact Cells ◽  
Phenol Sulfotransferase ◽  
Endogenous Metabolites ◽  
Direct Readout ◽  
Inorganic Sulfate

<p>The biological activation and incorporation of inorganic sulfate proceeds via a process known as sulfurylation. Transfer of a sulfuryl moiety from the activated sulfate donor, 3’-phosphoadenosine-5’-phosphosulfate (PAPS), to hydroxy-containing substrates by human phenol sulfotransferases (SULT1 family) alters substrate solubility and charge to affect the metabolism of endogenous metabolites, xenobiotics, and drugs. Current methods to monitor SULT1 activity in living cells primarily rely on radiolabeling and/or cell extractions, but these methods do not provide a direct readout of enzyme activity with a dynamic, temporally resolved spatial map in live, intact cells. To fill this gap, here, we present the development, computational modeling, <i>in vitro</i> enzymology, and biological application of Sulfotransferase Sensor-3, STS-3, an activity-based fluorescent sensor for SULT1A1, the most widely expressed and promiscuous SULT1 isoform. </p>

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

Quinolimide-based peptide biosensor for probing p25 in vitro and in living cells

2021 ◽  
pp. 129929
Author(s):  
Francisco Fueyo-González ◽  
Rosario Herranz ◽  
Simona Plesselova ◽  
Maria D. Giron ◽  
Rafael Salto ◽  
...  
Keyword(s):  
Living Cells

Fluorescent sensor based on triphenylamine for Zn2+ with high selectivity and imaging in living cells

2021 ◽  
Vol 251 ◽  
pp. 119480
Author(s):  
Dongyuan Wang ◽  
Qiang Yin ◽  
Ming Zheng ◽  
Yongbo Xie ◽  
Wen He ◽  
...  
Keyword(s):  
High Selectivity ◽  
Fluorescent Sensor ◽  
Living Cells

scholarly journals Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 386
Author(s):  
Ana Santos ◽  
Yongjun Jang ◽  
Inwoo Son ◽  
Jongseong Kim ◽  
Yongdoo Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Computational Modeling ◽  
Cardiac Tissue ◽  
Cardiac Development ◽  
Heart Tissue ◽  
Cardiac Tissue Engineering ◽  
Cardiac Cells

Cardiac tissue engineering aims to generate in vivo-like functional tissue for the study of cardiac development, homeostasis, and regeneration. Since the heart is composed of various types of cells and extracellular matrix with a specific microenvironment, the fabrication of cardiac tissue in vitro requires integrating technologies of cardiac cells, biomaterials, fabrication, and computational modeling to model the complexity of heart tissue. Here, we review the recent progress of engineering techniques from simple to complex for fabricating matured cardiac tissue in vitro. Advancements in cardiomyocytes, extracellular matrix, geometry, and computational modeling will be discussed based on a technology perspective and their use for preparation of functional cardiac tissue. Since the heart is a very complex system at multiscale levels, an understanding of each technique and their interactions would be highly beneficial to the development of a fully functional heart in cardiac tissue engineering.

A new in vitro micronucleus test in living cells associating biological tracers and high-content imaging

2016 ◽  
Vol 258 ◽  
pp. S146
Author(s):  
V. Graillot ◽  
O. Mondesert ◽  
T. Méténier ◽  
J. Vignard ◽  
V. Lobjois ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Living Cells ◽  
High Content Imaging

A reaction-based near-infrared fluorescent sensor for Cu2+ detection in aqueous buffer and its application in living cells and tissues imaging

2017 ◽  
Vol 94 ◽  
pp. 24-29 ◽  
Author(s):  
Huatang Zhang ◽  
Ling Feng ◽  
Yin Jiang ◽  
Yin-Ting Wong ◽  
Yonghe He ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Aqueous Buffer

I2 catalyzed access of spiro[indoline-3,4′-pyridine] appended amine dyad: new ON–OFF chemosensors for Cu2+ and imaging in living cells

2018 ◽  
Vol 16 (2) ◽  
pp. 302-315 ◽  
Author(s):  
Animesh Mondal ◽  
Barnali Naskar ◽  
Sanchita Goswami ◽  
Chandraday Prodhan ◽  
Keya Chaudhuri ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Cell Imaging ◽  
Colorimetric Detection ◽  
Living Cells ◽  
Fluorescent Cell

An efficient, easily tuneable route to construct a structurally diverse organic fluorescent probe and its applications towards the colorimetric detection of Cu2+ ions and in vitro fluorescent cell imaging of Cu2+ in HepG2 cells.

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