scholarly journals A live-cell assay for the detection of pre-microRNA–protein interactions

2021 ◽  
Author(s):  
Sydney L. Rosenblum ◽  
Daniel A. Lorenz ◽  
Amanda L. Garner
Keyword(s):  
Protein Interactions ◽  
Live Cell ◽  
Luciferase Assay ◽  
Cell Detection ◽  
Bioorthogonal Chemistry ◽  
Complementation Assay ◽  
Cell Assay ◽  
Rna Interaction ◽  
Split Luciferase

Leveraging bioorthogonal chemistry- and split-luciferase assay technologies, we have devised a new assay for the live-cell detection of RNA–protein interactions, RNA interaction with Protein-mediated Complementation Assay or RiPCA.

scholarly journals A Live-Cell Assay for the Detection of pre-microRNA-Protein Interactions

2020 ◽  
Author(s):  
Sydney L. Rosenblum ◽  
Daniel A. Lorenz ◽  
Amanda L. Garner
Keyword(s):  
Protein Interactions ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Live Cell ◽  
Cell Detection ◽  
Binding Domains ◽  
Genome Wide ◽  
Rna Interaction ◽  
And Function ◽  
Specific Sequences

AbstractRecent efforts in genome-wide sequencing and proteomics have revealed the fundamental roles that RNA-binding proteins (RBPs) play in the life cycle and function of both coding and non-coding RNAs. While these methodologies provide a systems-level view of the networking of RNA and proteins, approaches to enable the cellular validation of discovered interactions are lacking. Leveraging the power of bioorthogonal chemistry- and split-luciferase-based assay technologies, we have devised a conceptually new assay for the live-cell detection of RNA-protein interactions (RPIs), RNA interaction with Protein-mediated Complementation Assay, or RiPCA. As proof-of-concept, we have utilized the interaction of the pre-microRNA, pre-let-7, with its binding partner, Lin28. Using this system, we have demonstrated the selective detection of the pre-let-7-Lin28 RPI in both the cytoplasm and nucleus. Furthermore, we determined this technology can be used to discern relative affinities for specific sequences as well as of individual RNA binding domains. Thus, RiPCA has the potential to serve as a useful tool in supporting the investigation of cellular RPIs.

Live-cell assay to detect the dynamics of protein interactions

2006 ◽  
Author(s):  
Ignacio Demarco ◽  
Cynthia Booker ◽  
Richard Day
Keyword(s):  
Protein Interactions ◽  
Live Cell ◽  
Cell Assay

A split luciferase complementation assay for studying in vivo protein–protein interactions in filamentous ascomycetes

2012 ◽  
Vol 58 (3) ◽  
pp. 179-189 ◽  
Author(s):  
Hee-Kyoung Kim ◽  
Eun Ji Cho ◽  
Seong mi Jo ◽  
Bo Reum Sung ◽  
Seunghoon Lee ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

scholarly journals hFUT1-Based Live-Cell Assay To Profile α1-2-Fucoside-Enhanced Influenza Virus A Infection

2020 ◽  
Vol 15 (4) ◽  
pp. 819-823 ◽  
Author(s):  
Senlian Hong ◽  
Geramie Grande ◽  
Chenhua Yu ◽  
Digantkumar G. Chapla ◽  
Natalie Reigh ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Live Cell ◽  
Influenza Virus A ◽  
Cell Assay

scholarly journals Direct visualization of Bcl-2 family protein interactions using live cell fluorescent protein redistribution assays

2012 ◽  
Vol 3 (3) ◽  
pp. e288-e288 ◽  
Author(s):  
C Wong ◽  
D J Anderson ◽  
E F Lee ◽  
W D Fairlie ◽  
M J C Ludlam
Keyword(s):  
Protein Interactions ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Direct Visualization ◽  
Family Protein
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