scholarly journals Analysis of Protein‐Protein Interactions by Split Luciferase Complementation Assay

2019 ◽  
Vol 82 (1) ◽  
Author(s):  
Yuekun Lang ◽  
Zhong Li ◽  
Hongmin Li
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

A split luciferase complementation assay for studying in vivo protein–protein interactions in filamentous ascomycetes

2012 ◽  
Vol 58 (3) ◽  
pp. 179-189 ◽  
Author(s):  
Hee-Kyoung Kim ◽  
Eun Ji Cho ◽  
Seong mi Jo ◽  
Bo Reum Sung ◽  
Seunghoon Lee ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

LUCIFERASES FOR THE STUDY OF PROTEIN–PROTEIN INTERACTIONS IN LIVE CELLS AND ANIMALS

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 79-87 ◽  
Author(s):  
A. K. M. KAFI ◽  
MITSURU HATTORI ◽  
TAKEAKI OZAWA
Keyword(s):  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Protein Modification ◽  
Specific Protein ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Recent Developments ◽  
Split Luciferase Complementation ◽  
Spatio Temporal ◽  
Split Luciferase

Many imaging technologies based on luminescent proteins have proven useful for detecting protein–protein interactions, tracking cells in mice, and monitoring transcriptional regulation of specific genes. Especially, novel bioluminescent proteins have advanced the study of induced protein interactions and protein modification in live cells and animals. This review focuses on recent developments of bioluminescent probes for quantitative evaluation of specific protein–protein interactions and their spatio-temporal imaging by means of split luciferase complementation techniques. From the comparison between fluorescent and bioluminescent proteins, advantages and drawbacks of the bioluminescence techniques are described.

scholarly journals Identification of Compounds Targeting Hepatitis B Virus Core Protein Dimerization through a Split Luciferase Complementation Assay

2018 ◽  
Vol 62 (12) ◽  
Author(s):  
Xia-Fei Wei ◽  
Chun-Yang Gan ◽  
Jing Cui ◽  
Ying-Ying Luo ◽  
Xue-Fei Cai ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Protein Interactions ◽  
Core Protein ◽  
Cell Model ◽  
Protein Protein Interactions ◽  
B Virus ◽  
Split Luciferase Complementation ◽  
Split Luciferase

ABSTRACTThe capsid of the hepatitis B virus is an attractive antiviral target for developing therapies against chronic hepatitis B infection. Currently available core protein allosteric modulators (CpAMs) mainly affect one of the two major types of protein-protein interactions involved in the process of capsid assembly, namely, the interaction between the core dimers. Compounds targeting the interaction between two core monomers have not been rigorously screened due to the lack of screening models. We report here a cell-based assay in which the formation of core dimers is indicated by split luciferase complementation (SLC). Making use of this model, 2 compounds, Arbidol (umifenovir) and 20-deoxyingenol, were identified from a library containing 672 compounds as core dimerization regulators. Arbidol and 20-deoxyingenol inhibit the hepatitis B virus (HBV) DNA replicationin vitroby decreasing and increasing the formation of core dimer and capsid, respectively. Our results provided a proof of concept for the cell model to be used to screen new agents targeting the step of core dimer and capsid formation.

scholarly journals A Luciferase-fragment Complementation Assay to Detect Lipid Droplet-associated Protein-Protein Interactions

2016 ◽  
Vol 16 (3) ◽  
pp. 329-345 ◽  
Author(s):  
Petra Kolkhof ◽  
Michael Werthebach ◽  
Anna van de Venn ◽  
Gereon Poschmann ◽  
Lili Chen ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Fragment Complementation

Split Luciferase as an Optical Probe for Detecting Protein−Protein Interactions in Mammalian Cells Based on Protein Splicing

2001 ◽  
Vol 73 (11) ◽  
pp. 2516-2521 ◽  
Author(s):  
Takeaki Ozawa ◽  
Asami Kaihara ◽  
Moritoshi Sato ◽  
Kazunari Tachihara ◽  
Yoshio Umezawa
Keyword(s):  
Protein Interactions ◽  
Mammalian Cells ◽  
Optical Probe ◽  
Protein Splicing ◽  
Protein Protein Interactions ◽  
Split Luciferase
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