Live-cell assay to detect the dynamics of protein interactions

2006 ◽  
Author(s):  
Ignacio Demarco ◽  
Cynthia Booker ◽  
Richard Day
Keyword(s):  
Protein Interactions ◽  
Live Cell ◽  
Cell Assay

scholarly journals A live-cell assay for the detection of pre-microRNA–protein interactions

2021 ◽  
Author(s):  
Sydney L. Rosenblum ◽  
Daniel A. Lorenz ◽  
Amanda L. Garner
Keyword(s):  
Protein Interactions ◽  
Live Cell ◽  
Luciferase Assay ◽  
Cell Detection ◽  
Bioorthogonal Chemistry ◽  
Complementation Assay ◽  
Cell Assay ◽  
Rna Interaction ◽  
Split Luciferase

Leveraging bioorthogonal chemistry- and split-luciferase assay technologies, we have devised a new assay for the live-cell detection of RNA–protein interactions, RNA interaction with Protein-mediated Complementation Assay or RiPCA.

scholarly journals hFUT1-Based Live-Cell Assay To Profile α1-2-Fucoside-Enhanced Influenza Virus A Infection

2020 ◽  
Vol 15 (4) ◽  
pp. 819-823 ◽  
Author(s):  
Senlian Hong ◽  
Geramie Grande ◽  
Chenhua Yu ◽  
Digantkumar G. Chapla ◽  
Natalie Reigh ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Live Cell ◽  
Influenza Virus A ◽  
Cell Assay

scholarly journals Direct visualization of Bcl-2 family protein interactions using live cell fluorescent protein redistribution assays

2012 ◽  
Vol 3 (3) ◽  
pp. e288-e288 ◽  
Author(s):  
C Wong ◽  
D J Anderson ◽  
E F Lee ◽  
W D Fairlie ◽  
M J C Ludlam
Keyword(s):  
Protein Interactions ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Direct Visualization ◽  
Family Protein

scholarly journals Chemical toolbox for ‘live’ biochemistry to understand enzymatic functions in living systems

2019 ◽  
Author(s):  
Toru Komatsu ◽  
Yasuteru Urano
Keyword(s):  
Environmental Factors ◽  
Protein Interactions ◽  
Posttranslational Modifications ◽  
Live Cell ◽  
Living Systems ◽  
Protein Protein Interactions ◽  
Redox States ◽  
Recent Advances ◽  
Protein Functions ◽  
Research Tools

Abstract In this review, we present an overview of the recent advances in chemical toolboxes that are used to provide insights into ‘live’ protein functions in living systems. Protein functions are mediated by various factors inside of cells, such as protein−protein interactions, posttranslational modifications, and they are also subject to environmental factors such as pH, redox states and crowding conditions. Obtaining a true understanding of protein functions in living systems is therefore a considerably difficult task. Recent advances in research tools have allowed us to consider ‘live’ biochemistry as a valid approach to precisely understand how proteins function in a live cell context.

scholarly journals Detection and Characterization of Protein Interactions In Vivo by a Simple Live-Cell Imaging Method

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62195 ◽  
Author(s):  
Oriol Gallego ◽  
Tanja Specht ◽  
Thorsten Brach ◽  
Arun Kumar ◽  
Anne-Claude Gavin ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Method

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D

2007 ◽  
Vol 2 (1) ◽  
pp. 187-190 ◽  
Author(s):  
Ingrid Schmid ◽  
Christel Uittenbogaart ◽  
Beth D Jamieson
Keyword(s):  
Flow Cytometry ◽  
Actinomycin D ◽  
Live Cell ◽  
Hoechst 33342 ◽  
Cell Assay ◽  
Dual Laser

scholarly journals A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jie Shen ◽  
Changzu Cai ◽  
Zhilong Yu ◽  
Yuhong Pang ◽  
Ying Zhou ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Live Cell ◽  
Anthrax Toxin ◽  
Cell Assay ◽  
Cell Lethality

scholarly journals A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling

BMC Biology ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Edward R. Ballister ◽  
Jessica Rodgers ◽  
Franck Martial ◽  
Robert J. Lucas
Keyword(s):  
Live Cell ◽  
Cell Assay
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