Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B1

2020 ◽  
Vol 12 (43) ◽  
pp. 5229-5235
Author(s):  
Lingling Guo ◽  
Zhongxing Wang ◽  
Xinxin Xu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Rapid Detection ◽  
Detection Method ◽  
Fumonisin B1 ◽  
Immunochromatographic Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The developed time-resolved fluorescence immunochromatographic assay can be widely applicable as an ultrasensitive, rapid detection method for FB1 in grains.

A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

2020 ◽  
Vol 12 (3) ◽  
pp. 247-254 ◽  
Author(s):  
Xiaoxi Chang ◽  
Yaqing Zhang ◽  
Hebing Liu ◽  
Xiaoqi Tao
Keyword(s):  
Quantitative Determination ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Label Time

A quadruple-label time-resolved fluorescence immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detection of three mycotoxins (AFB1, ZEN and OTA) in 6 grains (corn, soybean, sorghum, wheat, rice and oats) was established.

A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

2019 ◽  
Vol 584 ◽  
pp. 113384 ◽  
Author(s):  
Michał Szkop ◽  
Kamil Brygoła ◽  
Magdalena Janczewska ◽  
Tomasz Ciach
Keyword(s):  
Quantitative Determination ◽  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

A Time-Resolved-Fluorescence Lateral Flow Assay for Rapid Detection of Cholyglycine Acid for the Diagnosis of Liver Diseases

2021 ◽  
Vol 11 (10) ◽  
pp. 2023-2029
Author(s):  
Wang Zhang ◽  
Yujun Zhou ◽  
Xiuli Xu ◽  
Yaping Tian ◽  
Chunyan Zhang
Keyword(s):  
Rapid Detection ◽  
Liver Diseases ◽  
Point Of Care ◽  
Quantitative Information ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Homogeneous Enzyme Immunoassay

Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum, plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.

Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

2017 ◽  
Vol 9 (45) ◽  
pp. 6430-6434 ◽  
Author(s):  
Yi Zhang ◽  
Xin-Liang Ding ◽  
Ming-Ming Guo ◽  
Ting-Ting Han ◽  
Zi-Jian Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

In the present study, a novel time-resolved fluorescence immunochromatographic assay was established for the rapid quantitative detection of microcystin-leucine arginine (MC-LR).

Two sensitive time-resolved fluoroimmunoassays for cellular fibronectin

1995 ◽  
Vol 41 (9) ◽  
pp. 1283-1287 ◽  
Author(s):  
J Kropf ◽  
A M Gressner
Keyword(s):  
Human Plasma ◽  
Detection Method ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Sandwich Type ◽  
Culture Supernatants ◽  
Cellular Fibronectin

Abstract Two sensitive sandwich-type immunoassays for determination of cellular fibronectin (cFN) in cell culture supernatants and in human plasma were developed. Both assays used a monoclonal antibody with specificity against the EDA sequence, which is characteristic for the cellular form of human and rat FN. Assay 1 involves binding of FN on gelatin-coated microwells followed by reaction with the anti-cFN antibody, whereas in assay 2 the anti-cFN antibody was immobilized first and detection was with an anti-FN antiserum. Time-resolved fluorescence spectrophotometry with measurement of an Eu3+ chelator after dissociation of the solid-phase complexes with urea/sodium dodecyl sulfate in the presence of excess Eu3+ was the detection method. The detection limit of the new assays was between 2.6 and 4.0 micrograms/L cFN. In serial dilution of human plasma samples, parallelism with the calibration curve was obtained over the whole measuring range (12-1000 micrograms/L) with assay 2, whereas assay 1 had deviations of the dilution curves at concentrations &gt; or = 100 micrograms/L. The between-run CVs for assays 1 and 2 were 11.4% and 7.2%, respectively, at a concentration of 200 micrograms/L (median value of 18 experiments). Respective within-series CVs of 4.3% and 4.7% were obtained at the same concentration. The recovery of added cFN from human plasma was between 90% and 96%.

Time-resolved fluorescence determination of albumin using ZnGeO:Mn luminescence nanorods modified with polydopamine nanoparticles

2021 ◽  
Vol 188 (12) ◽  
Author(s):  
Lifang Gao ◽  
Xu Zhang ◽  
Runlin Yang ◽  
Zhongwei Lv ◽  
Wenge Yang ◽  
...  
Keyword(s):  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Determination
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