Quantitative and rapid detection of microcystin-LR using time-resolved fluorescence immunochromatographic assay based on europium nanospheres

2017 ◽  
Vol 9 (45) ◽  
pp. 6430-6434 ◽  
Author(s):  
Yi Zhang ◽  
Xin-Liang Ding ◽  
Ming-Ming Guo ◽  
Ting-Ting Han ◽  
Zi-Jian Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved

In the present study, a novel time-resolved fluorescence immunochromatographic assay was established for the rapid quantitative detection of microcystin-leucine arginine (MC-LR).

A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

2020 ◽  
Vol 12 (3) ◽  
pp. 247-254 ◽  
Author(s):  
Xiaoxi Chang ◽  
Yaqing Zhang ◽  
Hebing Liu ◽  
Xiaoqi Tao
Keyword(s):  
Quantitative Determination ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Label Time

A quadruple-label time-resolved fluorescence immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detection of three mycotoxins (AFB1, ZEN and OTA) in 6 grains (corn, soybean, sorghum, wheat, rice and oats) was established.

Europium nanosphere-based fluorescence strip sensor for ultrasensitive and quantitative determination of fumonisin B1

2020 ◽  
Vol 12 (43) ◽  
pp. 5229-5235
Author(s):  
Lingling Guo ◽  
Zhongxing Wang ◽  
Xinxin Xu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Rapid Detection ◽  
Detection Method ◽  
Fumonisin B1 ◽  
Immunochromatographic Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

The developed time-resolved fluorescence immunochromatographic assay can be widely applicable as an ultrasensitive, rapid detection method for FB1 in grains.

scholarly journals Portable, Rapid, and Sensitive Time-Resolved Fluorescence Immunochromatography for On-Site Detection of Dexamethasone in Milk and Pork

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1339
Author(s):  
Xiangmei Li ◽  
Xiaomin Chen ◽  
Jinxiao Wu ◽  
Zhiwei Liu ◽  
Jin Wang ◽  
...  
Keyword(s):  
Technical Support ◽  
Rapid Screening ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Screening Methods ◽  
Time Resolved Fluorescence ◽  
Fluorescent Microspheres ◽  
Time Resolved ◽  
Coefficients Of Variation ◽  
Pork Samples

Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. In this study, a time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) integrated with a portable fluorescence reader was developed for the quantitative detection of DEX in milk and pork. The cut-off values of the TRFM-ICA were 0.25 ng/mL and 0.7 µg/kg, respectively. The limits of quantitation (LOQs) were 0.003 ng/mL and 0.062 µg/kg, respectively. The recovery rates were 80.0–106.7%, and 78.6–83.6%, respectively, with the coefficients of variation ranging 6.3–12.5%, and 7.5–10.3%, respectively. A parallel experiment for 20 milk and 10 pork samples with LC-MS/MS was carried out to confirm the performance of the on-site application of the developed TRFM-ICA. The results of the two methods are basically the same; the correlation (R2) was >0.98. The establishment of TRFM-ICA will provide a new sensitive and efficient technical support for the rapid screening of DEX in food.

A Time-Resolved-Fluorescence Lateral Flow Assay for Rapid Detection of Cholyglycine Acid for the Diagnosis of Liver Diseases

2021 ◽  
Vol 11 (10) ◽  
pp. 2023-2029
Author(s):  
Wang Zhang ◽  
Yujun Zhou ◽  
Xiuli Xu ◽  
Yaping Tian ◽  
Chunyan Zhang
Keyword(s):  
Rapid Detection ◽  
Liver Diseases ◽  
Point Of Care ◽  
Quantitative Information ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Homogeneous Enzyme Immunoassay

Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum, plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.

Immunochromatographic assay based on time‐resolved fluorescent nanobeads for the rapid detection of sulfamethazine in egg, honey, and pork

2020 ◽  
Vol 101 (2) ◽  
pp. 684-692
Author(s):  
Zexiang Wang ◽  
Song Hu ◽  
Huanhuan Bao ◽  
Keyu Xing ◽  
Jintao Liu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Time Resolved

scholarly journals A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W

2018 ◽  
Vol 13 ◽  
pp. 117727191875748 ◽  
Author(s):  
Biao Huang ◽  
Huixin Yu ◽  
Jiandong Bao ◽  
Manda Zhang ◽  
William L Green ◽  
...  
Keyword(s):  
Detection System ◽  
Polyclonal Antibodies ◽  
Maternal Serum ◽  
Quantitative Detection ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Serum Samples ◽  
Time Resolved ◽  
Detection Range ◽  
Wide Range

Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T2S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T2S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.

scholarly journals Novel Latex Microsphere Immunochromatographic Assay for Rapid Detection of Cadmium Ion in Asparagus

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 78
Author(s):  
Naifeng Xu ◽  
Qiaojuan Zhu ◽  
Jiangxiong Zhu ◽  
Jingze Jia ◽  
Xinlin Wei ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Limit Of Detection ◽  
High Specificity ◽  
Test Strip ◽  
Immunochromatographic Assay ◽  
Affinity Constant ◽  
Quantitative Detection ◽  
Cadmium Ion ◽  
Copper Transporter ◽  
Knock Out

Recently, concerns about heavy metal cadmium ion (Cd2+) residue in asparagus have been frequently reported, and there is an urgent need to develop an effective, sensitive, and rapid detection method for Cd2+. In this study, we innovatively combined molecular microbiology to carry out the comparative screening of Cd2+ chelators in a green, efficient, and specific way. The knock-out putative copper-transporter gene (pca1Δ) yeast strain with high sensitivity to Cd2+ was first used to screen the Cd2+ chelator, and the optimum chelator 1-(4-Isothiocyanatobenzyl)ethylenediamine-N,N,N,N′-tetraacetic acid (ITCBE) was obtained. Additionally, a rapid latex microsphere immunochromatographic assay (LMIA) was developed, based on the obtained monoclonal antibody (mAb) with high specificity and high affinity (affinity constant Ka = 1.83 × 1010 L/mol), to detect Cd2+ in asparagus. The 50% inhibitive concentration (IC50) of test strip was measured to be 0.2 ng/mL, and the limit of detection (IC10) for qualitative (LOD, for visual observation) and quantitative detection (LOQ, for data simulation) of the test strip was 2 ng/mL and 0.054 ng/mL, respectively. In all, the developed mAb-based LMIA shows a great potential for monitoring Cd2+ in asparagus, even in vegetable samples.

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