scholarly journals Mechanical response of adherent giant liposomes to indentation with a conical AFM-tip

Soft Matter ◽  
2015 ◽  
Vol 11 (22) ◽  
pp. 4487-4495 ◽  
Author(s):  
Edith Schäfer ◽  
Marian Vache ◽  
Torben-Tobias Kliesch ◽  
Andreas Janshoff
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Mechanical Response ◽  
Force Microscopy ◽  
Atomic Force

Mechanical properties of giant liposomes with actin cortices are determined with atomic force microscopy.

scholarly journals Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 468
Author(s):  
Maria Maares ◽  
Claudia Keil ◽  
Leif Löher ◽  
Andreas Weber ◽  
Amsatou Andorfer-Sarr ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Cell Lines ◽  
Mechanical Response ◽  
Cultured Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Impact ◽  
Ht 29

Monitoring biomechanics of cells or tissue biopsies employing atomic force microscopy (AFM) offers great potential to identify diagnostic biomarkers for diseases, such as colorectal cancer (CRC). Data on the mechanical properties of CRC cells, however, are still scarce. There is strong evidence that the individual zinc status is related to CRC risk. Thus, this study investigates the impact of differing zinc supply on the mechanical response of the in vitro CRC cell lines HT-29 and HT-29-MTX during their early proliferation (24–96 h) by measuring elastic modulus, relaxation behavior, and adhesion factors using AFM. The differing zinc supply severely altered the proliferation of these cells and markedly affected their mechanical properties. Accordingly, zinc deficiency led to softer cells, quantitatively described by 20–30% lower Young’s modulus, which was also reflected by relevant changes in adhesion and rupture event distribution compared to those measured for the respective zinc-adequate cultured cells. These results demonstrate that the nutritional zinc supply severely affects the nanomechanical response of CRC cell lines and highlights the relevance of monitoring the zinc content of cancerous cells or biopsies when studying their biomechanics with AFM in the future.

Mechanical Properties of Membrane Surface of Cultured Astrocyte Revealed by Atomic Force Microscopy

2000 ◽  
Vol 39 (Part 1, No. 6B) ◽  
pp. 3711-3716 ◽  
Author(s):  
Hatsuki Shiga ◽  
Yukako Yamane ◽  
Etsuro Ito ◽  
Kazuhiro Abe ◽  
Kazushige Kawabata ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Membrane Surface ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Corrosion Behavior and Mechanical Properties of a Nanocomposite Superhydrophobic Coating

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 652
Author(s):  
Divine Sebastian ◽  
Chun-Wei Yao ◽  
Lutfun Nipa ◽  
Ian Lian ◽  
Gary Twu
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Nanocomposite Coating ◽  
Superhydrophobic Coating ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Images ◽  
Scanning Electron

In this work, a mechanically durable anticorrosion superhydrophobic coating is developed using a nanocomposite coating solution composed of silica nanoparticles and epoxy resin. The nanocomposite coating developed was tested for its superhydrophobic behavior using goniometry; surface morphology using scanning electron microscopy and atomic force microscopy; elemental composition using energy dispersive X-ray spectroscopy; corrosion resistance using atomic force microscopy; and potentiodynamic polarization measurements. The nanocomposite coating possesses hierarchical micro/nanostructures, according to the scanning electron microscopy images, and the presence of such structures was further confirmed by the atomic force microscopy images. The developed nanocomposite coating was found to be highly superhydrophobic as well as corrosion resistant, according to the results from static contact angle measurement and potentiodynamic polarization measurement, respectively. The abrasion resistance and mechanical durability of the nanocomposite coating were studied by abrasion tests, and the mechanical properties such as reduced modulus and Berkovich hardness were evaluated with the aid of nanoindentation tests.

scholarly journals The Infuence of Salicin on Rheological and Film-Forming Properties of Collagen

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1661
Author(s):  
Katarzyna Adamiak ◽  
Katarzyna Lewandowska ◽  
Alina Sionkowska
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Infrared Spectroscopy ◽  
Rheological Properties ◽  
Silver Carp ◽  
Shear Tests ◽  
Force Microscopy ◽  
Atomic Force ◽  
Film Forming ◽  
Potential Applications

Collagen films are widely used as adhesives in medicine and cosmetology. However, its properties require modification. In this work, the influence of salicin on the properties of collagen solution and films was studied. Collagen was extracted from silver carp skin. The rheological properties of collagen solutions with and without salicin were characterized by steady shear tests. Thin collagen films were prepared by solvent evaporation. The structure of films was researched using infrared spectroscopy. The surface properties of films were investigated using Atomic Force Microscopy (AFM). Mechanical properties were measured as well. It was found that the addition of salicin modified the roughness of collagen films and their mechanical and rheological properties. The above-mentioned parameters are very important in potential applications of collagen films containing salicin.

scholarly journals Anisotropic Mechanical Properties of Living Cells Revealed by Integrated Spinning Disk Confocal and Atomic Force Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spinning Disk ◽  
Anisotropic Mechanical Properties

scholarly journals Resolving Structure and Mechanical Properties at the Nanoscale of Viruses with Frequency Modulation Atomic Force Microscopy

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30204 ◽  
Author(s):  
David Martinez-Martin ◽  
Carolina Carrasco ◽  
Mercedes Hernando-Perez ◽  
Pedro J. de Pablo ◽  
Julio Gomez-Herrero ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Frequency Modulation ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals MORPHOLOGICAL AND NANOMECHANICAL INVESTIGATIONS OF MAGNEVIST NANO-ENCAPSULATED DENDRIMERS BY ATOMIC FORCE MICROSCOPY

2012 ◽  
Vol 1 ◽  
Author(s):  
Hosam Gharib Abdelhady
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Magnetic Resonance ◽  
Magnetic Resonance Images ◽  
Spherical Particles ◽  
Contrast Imaging ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polyamidoamine Dendrimers ◽  
Molecular Morphology

Objectives: This research aims at investigating the effect of nano-encapsulating the MagnevistTM, a magnetic resonance imaging agent, within generation four, 1, 4- diaminobutane core polyamidoamine dendrimers on their molecular morphology and their nano-mechanical properties in liquid.Methods: Atomic force microscopy was applied in its imaging and force measuring modes to investigate, on the molecular scale, the morphological and nano-mechanical changes in generation four, 1, 4-diaminobutane core polyamidoamine dendrimers due to the nano-encapsulation of Magnevist in liquid.Results: The weight gain of dendrimers indicates the loading of ~ 30 Magnevist molecules. This has increased the rigidity of the dendrimer molecules, compared to unloaded dendrimers. Atomic force microscopy showed individual well-defined nano-spherical particles with nanoscopic dimensions of (40±13 nm in diameter and 4.38±0.54 nm in height). In contrast, imaging of non encapsulated dendrimers revealed loose aggregates of 15±3.5 nm in diameter and 0.9±0.2 nm in height.Conclusions: The atomic force microscopy, in liquid, was successfully applied to differentiate between Magnevist nano-encapsulated and non-encapsulated dendrimers, in their morphology and in their nano-mechanical properties. The results confirm the nano-encapsulation of Magnevist within generation four, 1,4-diaminobutane core polyamidoamine dendrimers. This loading increased the rigidity of the nanoencapsulated dendrimer, packed ~ 9 Magnevist-G 4 molecules together and may probably enhance the magnetic resonance images and increase their duration of time in the bloodstream when compared with Magnevist alone. Thus elongating the imaging sessions without the need for additional contrast agent doses.

Sign in / Sign up

Export Citation Format

Share Document