Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

2020 ◽  
Vol 12 (26) ◽  
pp. 3361-3367
Author(s):  
Wenshuai Li ◽  
Guorui Wu ◽  
Min Wang ◽  
Aiqin Yue ◽  
Weijun Du ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Naked Eye ◽  
Class A

We propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments.

Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction

2015 ◽  
Vol 51 (16) ◽  
pp. 3371-3374 ◽  
Author(s):  
Fengxia Su ◽  
Limei Wang ◽  
Yueying Sun ◽  
Chenghui Liu ◽  
Xinrui Duan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genomic Dna ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Cpg Methylation ◽  
Chain Reaction ◽  
Selective Method ◽  
Ligase Chain Reaction ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Using LCR amplification and AuNP-based colorimetric detection, a highly sensitive and selective method for detection of DNA methylation has been developed.

scholarly journals Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Congcong Yin ◽  
Qiaoling Zhao ◽  
Aiqin Yue ◽  
Weijun Du ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Colorimetric Detection ◽  
High Specificity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Class A ◽  
Key Enzyme ◽  
Hairpin Probes ◽  
Type Ab ◽  
Triton X

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

High-throughput and ultratrace naked-eye colorimetric detection of Au3+ based on the gold amalgam-stimulated peroxidase mimetic activity in aqueous solutions

2017 ◽  
Vol 53 (36) ◽  
pp. 5056-5058 ◽  
Author(s):  
Shouting Zhang ◽  
Zhuoyue Zhang ◽  
Tiansheng Wang ◽  
Dongxu Zhang ◽  
Xuemei Li ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
High Throughput ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Label Free ◽  
Efficient Strategy ◽  
Naked Eye ◽  
Peroxidase Mimetic

Herein, we present a catalysis-based, label-free, and efficient strategy for a rapid, high-throughput, highly selective and ultrasensitive naked-eye colorimetric assay of Au3+ in aqueous solutions, based on the gold amalgam-stimulated peroxidase mimetic activity.

scholarly journals Pt Nanoparticles Anchored on NH2-MIL-101 with Efficient Peroxidase-Like Activity for Colorimetric Detection of Dopamine

Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 140
Author(s):  
Jing Li ◽  
Keying Xu ◽  
Yang Chen ◽  
Jie Zhao ◽  
Peiyao Du ◽  
...  
Keyword(s):  
Active Sites ◽  
Clinical Medicine ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Pt Nanoparticles ◽  
Robust Detection ◽  
Color Changes ◽  
Relevant Role ◽  
Metal Organic ◽  
The Central Nervous System

Dopamine (DA) is an important catecholamine neurotransmitter that plays a highly relevant role in regulating the central nervous system, and abnormal DA content can cause many immune-related diseases. Hence, it is of significance to sensitively and specifically identify DA for clinical medicine. In this work, Pt/NH2-MIL-101 hybrid nanozymes with bimetallic catalytic centers were fabricated by forming coordinate bonds between Pt nanoparticles (Pt NPs) and –NH2 on metal–organic frameworks (MOF). The catalytic activity of Pt/NH2-MIL-101 was increased by 1.5 times via enlarging the exposure of more active sites and improving the activity of the active sites through the strategy of forming bimetallic catalytic centers. In the presence of DA, competing with 3, 3′, 5, 5′-tetramethylbenzidine (TMB) for the generated hydroxyl radicals (•OH), the blue oxidation state TMB (Ox-TMB) is reduced to colorless TMB, showing dramatic color changes. The Pt/NH2-MIL-101-based colorimetric assay enables the sensitive and robust detection of DA molecules with a detection limit of only 0.42 μM and has an observable potential in clinical applications.

Naked-eye colorimetric detection of HCV RNA mediated by a 5′ UTR-targeted antisense oligonucleotide and plasmonic gold nanoparticles

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta
Keyword(s):  
Gold Nanoparticles ◽  
Antisense Oligonucleotide ◽  
Early Stage ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Naked Eye

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.

scholarly journals Comparison of the ligase chain reaction with cell culture for the diagnosis of Chlamydia trachomatis infection in women.

1996 ◽  
Vol 49 (2) ◽  
pp. 116-119 ◽  
Author(s):  
G L Ridgway ◽  
G Mumtaz ◽  
A J Robinson ◽  
M Franchini ◽  
C Carder ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chlamydia Trachomatis ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection

An ultra-sensitive colorimetric assay for reliable visual detection of telomerase activity

The Analyst ◽  
2017 ◽  
Vol 142 (17) ◽  
pp. 3235-3240 ◽  
Author(s):  
Yaocai Wang ◽  
Luzhu Yang ◽  
Yanjun Wang ◽  
Wei Liu ◽  
Baoxin Li ◽  
...  
Keyword(s):  
Hela Cell ◽  
Visual Detection ◽  
Colorimetric Assay ◽  
Telomerase Activity ◽  
Cell Lysates ◽  
Naked Eye ◽  
Vis Spectroscopy

We proposed a sensitive colorimetric assay for detecting telomerase activity. The telomerase activity of 5 and 20 HeLa cell lysates can be detected via UV-vis spectroscopy and the naked eye, respectively.

Ligase Chain Reaction

Biologicals ◽  
1996 ◽  
Vol 24 (3) ◽  
pp. 197-199 ◽  
Author(s):  
Helen H. Lee
Keyword(s):  
Chain Reaction ◽  
Ligase Chain Reaction
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