scholarly journals Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

2020 ◽  
Vol 12 (21) ◽  
pp. 2674-2681
Author(s):  
Fatemeh Shafiei ◽  
Kathleen McAuliffe ◽  
Yousef Bagheri ◽  
Zhining Sun ◽  
Qikun Yu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Rapid Detection ◽  
Rna Aptamer ◽  
Label Free ◽  
Target Analytes ◽  
Label Free Detection

A paper-based portable fluorogenic RNA sensor for the selective, sensitive, and rapid detection of target analytes.

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Optical Biosensors for Label-Free Detection of Small Molecules

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4126 ◽  
Author(s):  
Riikka Peltomaa ◽  
Bettina Glahn-Martínez ◽  
Elena Benito-Peña ◽  
María Moreno-Bondi
Keyword(s):  
Small Molecules ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Optical Biosensors ◽  
Label Free ◽  
Fiber Sensors ◽  
Label Free Detection ◽  
Surface Enhanced Raman ◽  
Resonance Surface ◽  
Small Molecule Detection

Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.

scholarly journals Multimodal label-free detection and discrimination for small molecules using a nanoporous resonator

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Jinsung Park ◽  
Doyeon Bang ◽  
Kuewhan Jang ◽  
Eunkyoung Kim ◽  
Seungjoo Haam ◽  
...  
Keyword(s):  
Small Molecules ◽  
Label Free ◽  
Label Free Detection

scholarly journals Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures

2019 ◽  
Vol 10 ◽  
pp. 967-974
Author(s):  
Jad Sabek ◽  
Francisco Javier Díaz-Fernández ◽  
Luis Torrijos-Morán ◽  
Zeneida Díaz-Betancor ◽  
Ángel Maquieira ◽  
...  
Keyword(s):  
Binding Sites ◽  
Photonic Bandgap ◽  
Near Field ◽  
Evanescent Field ◽  
Label Free ◽  
Target Analytes ◽  
Label Free Detection ◽  
Exponential Decrease ◽  
Structure Surface ◽  
Photonic Bandgap Structures

A photonic bandgap (PBG) biosensor has been developed for the label-free detection of proteins. As the sensing in this type of structures is governed by the interaction between the evanescent field going into the cladding and the target analytes, scanning near-field optical microscopy has been used to characterize the profile of that evanescent field. The study confirms the strong exponential decrease of the signal as it goes into the cladding. This means that biorecognition events must occur as close to the PBG structure surface as possible in order to obtain the maximum sensing response. Within this context, the PBG biosensor has been biofunctionalized with half-antibodies specific to bovine serum albumin (BSA) using a UV-induced immobilization procedure. The use of half-antibodies allows one to reduce the thickness of the biorecognition volume down to ca. 2.5 nm, thus leading to a higher interaction with the evanescent field, as well as a proper orientation of their binding sites towards the target sample. Then, the biofunctionalized PBG biosensor has been used to perform a direct and real-time detection of the target BSA antigen.

scholarly journals A gold nanopopcorn attached single-walled carbon nanotube hybrid for rapid detection and killing of bacteria

2014 ◽  
Vol 2 (43) ◽  
pp. 7534-7543 ◽  
Author(s):  
Thomas J. Ondera ◽  
Ashton T. Hamme II
Keyword(s):  
Carbon Nanotube ◽  
Rapid Detection ◽  
Label Free ◽  
Hybrid Nanomaterials ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Label Free Detection ◽  
Selective Label

Antibody conjugated gold nanopopcorn attached SWCNT hybrid nanomaterials possess great potential for selective label-free detection and photothermal killing of bacteria.

Gold nanoparticle integrated artificial nanochannels for label-free detection of peroxynitrite

2021 ◽  
Author(s):  
Jing Wu ◽  
Xing Wang ◽  
Lei Ge ◽  
Rui Lv ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Small Molecules ◽  
Gold Nanoparticle ◽  
Sensitive Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Highly Sensitive Detection

A label-free method for rapid and highly sensitive detection of ONOO− was proposed by employing ABEI@AuNPs integrated nanochannels. This work paves a new way to develop a versatile platform for the detection of different biological small molecules.

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 680 ◽  
Author(s):  
Cao-An Vu ◽  
Wen-Yih Chen
Keyword(s):  
Small Molecules ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Field Effect Transistors ◽  
High Specificity ◽  
Label Free ◽  
Sensing Technology ◽  
Wide Range ◽  
Label Free Detection ◽  
Effect Transistor

Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, viruses, and cells. The outburst of field-effect transistors provides a label-free detection and ultra-sensitive technique with significantly improved results in terms of detection of substances. However, their combination in this field is challenged by several factors. Recent advances in the discovery of aptamers and studies of Field-Effect Transistor (FET) aptasensors overcome these limitations and potentially expand the dominance of aptamers in the biosensor market.

Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

2017 ◽  
Vol 9 (20) ◽  
pp. 3055-3060 ◽  
Author(s):  
Kefeng Wu ◽  
Changbei Ma ◽  
Haisheng Liu ◽  
Hailun He ◽  
Weimin Zeng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Rnase H ◽  
Fluorescence Assay ◽  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

A simple fluorescence based biosensor for label-free detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates is reported.

Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase

2007 ◽  
Vol 23 (4) ◽  
pp. 459-465 ◽  
Author(s):  
Kyo Seon Hwang ◽  
Sang-Myung Lee ◽  
Kilho Eom ◽  
Jeong Hoon Lee ◽  
Yoon-Sik Lee ◽  
...  
Keyword(s):  
Vibration Modes ◽  
Rna Aptamer ◽  
Label Free ◽  
Label Free Detection
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