scholarly journals Multimodal label-free detection and discrimination for small molecules using a nanoporous resonator

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Jinsung Park ◽  
Doyeon Bang ◽  
Kuewhan Jang ◽  
Eunkyoung Kim ◽  
Seungjoo Haam ◽  
...  
Keyword(s):  
Small Molecules ◽  
Label Free ◽  
Label Free Detection

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

2020 ◽  
Vol 12 (21) ◽  
pp. 2674-2681
Author(s):  
Fatemeh Shafiei ◽  
Kathleen McAuliffe ◽  
Yousef Bagheri ◽  
Zhining Sun ◽  
Qikun Yu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Rapid Detection ◽  
Rna Aptamer ◽  
Label Free ◽  
Target Analytes ◽  
Label Free Detection

A paper-based portable fluorogenic RNA sensor for the selective, sensitive, and rapid detection of target analytes.

scholarly journals Optical Biosensors for Label-Free Detection of Small Molecules

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4126 ◽  
Author(s):  
Riikka Peltomaa ◽  
Bettina Glahn-Martínez ◽  
Elena Benito-Peña ◽  
María Moreno-Bondi
Keyword(s):  
Small Molecules ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Optical Biosensors ◽  
Label Free ◽  
Fiber Sensors ◽  
Label Free Detection ◽  
Surface Enhanced Raman ◽  
Resonance Surface ◽  
Small Molecule Detection

Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.

Gold nanoparticle integrated artificial nanochannels for label-free detection of peroxynitrite

2021 ◽  
Author(s):  
Jing Wu ◽  
Xing Wang ◽  
Lei Ge ◽  
Rui Lv ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Small Molecules ◽  
Gold Nanoparticle ◽  
Sensitive Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Highly Sensitive Detection

A label-free method for rapid and highly sensitive detection of ONOO− was proposed by employing ABEI@AuNPs integrated nanochannels. This work paves a new way to develop a versatile platform for the detection of different biological small molecules.

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 680 ◽  
Author(s):  
Cao-An Vu ◽  
Wen-Yih Chen
Keyword(s):  
Small Molecules ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Field Effect Transistors ◽  
High Specificity ◽  
Label Free ◽  
Sensing Technology ◽  
Wide Range ◽  
Label Free Detection ◽  
Effect Transistor

Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, viruses, and cells. The outburst of field-effect transistors provides a label-free detection and ultra-sensitive technique with significantly improved results in terms of detection of substances. However, their combination in this field is challenged by several factors. Recent advances in the discovery of aptamers and studies of Field-Effect Transistor (FET) aptasensors overcome these limitations and potentially expand the dominance of aptamers in the biosensor market.

Tunable Plasmonic Cavity for Label-free Detection of Small Molecules

2018 ◽  
Vol 10 (15) ◽  
pp. 13226-13235 ◽  
Author(s):  
Jung A Kwon ◽  
Chang Min Jin ◽  
Yonghee Shin ◽  
Hye Young Kim ◽  
Yura Kim ◽  
...  
Keyword(s):  
Small Molecules ◽  
Label Free ◽  
Label Free Detection

Optical sensors with molecularly imprinted nanospheres: a promising approach for robust and label-free detection of small molecules

2011 ◽  
Vol 402 (10) ◽  
pp. 3245-3252 ◽  
Author(s):  
Felix Kolarov ◽  
Klaus Niedergall ◽  
Monika Bach ◽  
Günter E. M. Tovar ◽  
Günter Gauglitz
Keyword(s):  
Small Molecules ◽  
Optical Sensors ◽  
Label Free ◽  
Molecularly Imprinted ◽  
Label Free Detection

scholarly journals Highly Sensitive Label-Free Detection of Small Molecules with an Optofluidic Microbubble Resonator

Micromachines ◽  
2018 ◽  
Vol 9 (6) ◽  
pp. 274 ◽  
Author(s):  
Zihao Li ◽  
Chenggang Zhu ◽  
Zhihe Guo ◽  
Bowen Wang ◽  
Xiang Wu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection

scholarly journals Spectral-Phase Interferometry Detection of Ochratoxin A via Aptamer-Functionalized Graphene Coated Glass

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 226
Author(s):  
Nikita Nekrasov ◽  
Natalya Yakunina ◽  
Averyan V. Pushkarev ◽  
Alexey V. Orlov ◽  
Ivana Gadjanski ◽  
...  
Keyword(s):  
Small Molecules ◽  
Ochratoxin A ◽  
Direct Method ◽  
Low Cost ◽  
Optical Signal ◽  
Spectral Phase ◽  
Urea Solution ◽  
Label Free ◽  
Label Free Detection ◽  
Interferometric Sensor

In this work, we report a novel method of label-free detection of small molecules based on direct observation of interferometric signal change in graphene-modified glasses. The interferometric sensor chips are fabricated via a conventional wet transfer method of CVD-grown graphene onto the glass coverslips, lowering the device cost and allowing for upscaling the sensor fabrication. For the first time, we report the use of graphene functionalized by the aptamer as the bioreceptor, in conjunction with Spectral-Phase Interferometry (SPI) for detection of ochratoxin A (OTA). In a direct assay with an OTA-specific aptamer, we demonstrated a quick and significant change of the optical signal in response to the maximum tolerable level of OTA concentration. The sensor regeneration is possible in urea solution. The developed platform enables a direct method of kinetic analysis of small molecules using a low-cost optical chip with a graphene-aptamer sensing layer.

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