scholarly journals Lateral flow immunochromatographic assay on a single piece of paper

The Analyst ◽  
2021 ◽  
Author(s):  
Xue Jiang ◽  
Peter B. Lillehoj
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Cellulose Paper ◽  
Single Piece ◽  
Analyte Detection ◽  
Lateral Flow Immunochromatographic Assay

A lateral flow immunochromatographic assay constructed from a single piece of cellulose paper for rapid analyte detection.

scholarly journals Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiayi Wang ◽  
Jinyu Zhou ◽  
Yiqiang Chen ◽  
Xinpei Zhang ◽  
Yongpeng Jin ◽  
...  
Keyword(s):  
Animal Feed ◽  
Animal Production ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Animal Origin ◽  
Quantitative Detection ◽  
One Step ◽  
Assay Time ◽  
Animal Origin Food ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background Colistin (polymyxin E) is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention, treatment, and growth promotion. However, the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings, and its residue in animal-origin food may also pose serious health hazards to humans. Thus, it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food. Results A one-step enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic assay (LFIA) for colistin were developed based on a newly developed monoclonal antibody. The ELISA showed a 50% inhibition value (IC50) of 9.7 ng/mL with assay time less than 60 min, while the LFIA had a strip reader-based detection limit of 0.87 ng/mL in phosphate buffer with assay time less than 15 min. For reducing the non-specific adsorption of colistin onto sample vial, the components of sample extraction solution were optimized and proved to greatly improve the assay accuracy. The spiked recovery experiment showed that the recoveries of colistin from feed, milk and meat samples were in the range of 77.83% to 113.38% with coefficient of variations less than 13% by ELISA analysis and less than 18% by LFIA analysis, respectively. Furthermore, actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental analysis. Conclusions The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food.

Improvement of the stability of immunochromatographic assay for the quantitative detection of clenbuterol in swine urine

2014 ◽  
Vol 6 (18) ◽  
pp. 7394-7398 ◽  
Author(s):  
Tao Peng ◽  
Wan-chun Yang ◽  
Wei-Hua Lai ◽  
Yong-Hua Xiong ◽  
Hua Wei ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Swine Urine ◽  
The Stability ◽  
Lateral Flow Immunochromatographic Assay

The stability of lateral-flow immunochromatographic assay for quantitative detection of clenbuterol was studied and improved.

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