Improvement of the stability of immunochromatographic assay for the quantitative detection of clenbuterol in swine urine

2014 ◽  
Vol 6 (18) ◽  
pp. 7394-7398 ◽  
Author(s):  
Tao Peng ◽  
Wan-chun Yang ◽  
Wei-Hua Lai ◽  
Yong-Hua Xiong ◽  
Hua Wei ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Swine Urine ◽  
The Stability ◽  
Lateral Flow Immunochromatographic Assay

The stability of lateral-flow immunochromatographic assay for quantitative detection of clenbuterol was studied and improved.

scholarly journals Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiayi Wang ◽  
Jinyu Zhou ◽  
Yiqiang Chen ◽  
Xinpei Zhang ◽  
Yongpeng Jin ◽  
...  
Keyword(s):  
Animal Feed ◽  
Animal Production ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Animal Origin ◽  
Quantitative Detection ◽  
One Step ◽  
Assay Time ◽  
Animal Origin Food ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background Colistin (polymyxin E) is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention, treatment, and growth promotion. However, the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings, and its residue in animal-origin food may also pose serious health hazards to humans. Thus, it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food. Results A one-step enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic assay (LFIA) for colistin were developed based on a newly developed monoclonal antibody. The ELISA showed a 50% inhibition value (IC50) of 9.7 ng/mL with assay time less than 60 min, while the LFIA had a strip reader-based detection limit of 0.87 ng/mL in phosphate buffer with assay time less than 15 min. For reducing the non-specific adsorption of colistin onto sample vial, the components of sample extraction solution were optimized and proved to greatly improve the assay accuracy. The spiked recovery experiment showed that the recoveries of colistin from feed, milk and meat samples were in the range of 77.83% to 113.38% with coefficient of variations less than 13% by ELISA analysis and less than 18% by LFIA analysis, respectively. Furthermore, actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental analysis. Conclusions The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food.

scholarly journals Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyung Mi Park ◽  
Da Jung Chung ◽  
Mijin Choi ◽  
Taejoon Kang ◽  
Jinyoung Jeong
Keyword(s):  
Chemical Species ◽  
Test Strip ◽  
Disease Diagnosis ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Lateral Flow Immunochromatographic Assay

Abstract A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

Sample Preincubation Strategy for Sensitive and Quantitative Detection of Clenbuterol in Swine Urine Using a Fluorescent Microsphere–Based Immunochromatographic Assay

2014 ◽  
Vol 77 (11) ◽  
pp. 1998-2003 ◽  
Author(s):  
SHENG L. DENG ◽  
SHAN SHAN ◽  
CHAO L. XU ◽  
DAO F. LIU ◽  
YONG H. XIONG ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Rapid Screening ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescent Microsphere ◽  
Swine Urine ◽  
Relative Standard

We describe an ultrasensitive and quantitative immunochromatographic assay to determine the amount of clenbuterol (CLB) in swine urine. In this study, fluorescein isothiocyanate polystyrene fluorescent microspheres were used as probes. A sample preincubation strategy was introduced to this immunochromatographic assay. Results showed that the strategy evidently improved the sensitivity and accuracy of lateral flow assay. The method was completed in 20 min, and a half-maximal inhibitory concentration of 0.13 μg liter−1 was obtained. The limit of detection of the proposed method to determine CLB in swine urine was 0.01 μg liter−1, which was lower than the limit of detection of immunochromatographic assays without preincubation. Intra-and interday recoveries of spiked swine urine ranged from 85.0 to 107.5%. The relative standard deviation values of the preincubated test strip ranged from 2.7 to 12.5%. Analysis of the CLB in swine urine samples showed that the result obtained from the lateral flow assay is consistent with that obtained from a commercial enzyme-linked immunosorbent assay kit. Our results suggest that the developed fluorescent microsphere–based immunochromatographic assay may be useful as a rapid screening method to detect CLB quantitatively.

scholarly journals Fluorescent microsphere immunochromatographic assays for detecting bone alkaline phosphatase based on biolayer interferometry-selected antibody

RSC Advances ◽  
2017 ◽  
Vol 7 (52) ◽  
pp. 32952-32959 ◽  
Author(s):  
Miao Liu ◽  
Li-Feng Zeng ◽  
Ya-Jie Yang ◽  
Li-Ming Hu ◽  
Wei-Hua Lai
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Biolayer Interferometry ◽  
Fluorescent Microsphere ◽  
Highly Sensitive ◽  
First Time ◽  
Lateral Flow Immunochromatographic Assay

A convenient, reliable, highly sensitive, and competitive fluorescent microsphere-lateral flow immunochromatographic assay (FM-LFIA) was developed for the quantitative detection of BAP for the first time.

Sign in / Sign up

Export Citation Format

Share Document