Rapid quantification of coliforms in ready‐to‐eat foods using lateral‐flow immunochromatographic assay

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Tatsuya Tominaga
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunochromatographic Assay ◽  
Rapid Quantification

scholarly journals Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jiayi Wang ◽  
Jinyu Zhou ◽  
Yiqiang Chen ◽  
Xinpei Zhang ◽  
Yongpeng Jin ◽  
...  
Keyword(s):  
Animal Feed ◽  
Animal Production ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Animal Origin ◽  
Quantitative Detection ◽  
One Step ◽  
Assay Time ◽  
Animal Origin Food ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background Colistin (polymyxin E) is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention, treatment, and growth promotion. However, the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings, and its residue in animal-origin food may also pose serious health hazards to humans. Thus, it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food. Results A one-step enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic assay (LFIA) for colistin were developed based on a newly developed monoclonal antibody. The ELISA showed a 50% inhibition value (IC50) of 9.7 ng/mL with assay time less than 60 min, while the LFIA had a strip reader-based detection limit of 0.87 ng/mL in phosphate buffer with assay time less than 15 min. For reducing the non-specific adsorption of colistin onto sample vial, the components of sample extraction solution were optimized and proved to greatly improve the assay accuracy. The spiked recovery experiment showed that the recoveries of colistin from feed, milk and meat samples were in the range of 77.83% to 113.38% with coefficient of variations less than 13% by ELISA analysis and less than 18% by LFIA analysis, respectively. Furthermore, actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental analysis. Conclusions The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food.

Improvement of the stability of immunochromatographic assay for the quantitative detection of clenbuterol in swine urine

2014 ◽  
Vol 6 (18) ◽  
pp. 7394-7398 ◽  
Author(s):  
Tao Peng ◽  
Wan-chun Yang ◽  
Wei-Hua Lai ◽  
Yong-Hua Xiong ◽  
Hua Wei ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
Swine Urine ◽  
The Stability ◽  
Lateral Flow Immunochromatographic Assay

The stability of lateral-flow immunochromatographic assay for quantitative detection of clenbuterol was studied and improved.

scholarly journals Diagnostic Value of Bronchoalveolar Lavage Fluid Cryptococcal Antigen-Lateral Flow Immunochromatographic Assay for Pulmonary Cryptococcosis in non-HIV Patients

2020 ◽  
Author(s):  
Hui-Qing Zeng ◽  
Xue-Ying Cai ◽  
Xiao-Bin Zhang ◽  
Dong-Yong Yang ◽  
Li Lin ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Pulmonary Cryptococcosis ◽  
Cryptococcal Antigen ◽  
Positive Results ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background: The present study aimed to investigate the diagnostic value of cryptococcal antigen-lateral flow immunochromatographic assay (CrAg-LFA) in bronchoalveolar lavage fluid (BALF) of patients with pulmonary cryptococcosis (PC). Methods: The subjects collected from March 2015 to October 2018 in 4 hospitals were divided into the pulmonary cryptococcosis (PC) group (n=72) and the non-pulmonary cryptococcosis (non-PC) group (n=236). The patients in the PC group were diagnosed by lung biopsy. Results: The sensitivity, specificity, positive and negative predicted values of CrAg-LFA in the serum were 75.0%, 99.6%, 98.2%, and 92.9%, respectively, while those in the BALF were 93.1%, 100.0%, 100.0%, and 97.9%, respectively. Hence, the sensitivity of the CrAg-LFA in BALF was significantly higher than that in the serum of the patients in the PC group (P<0.05). Conclusion: The CrAg-LFA is a rapid, simple and safe experimental method. CrAg-LFA in BALF samples can offer higher diagnostic value for PC than that noted in the serum samples of PC subjects. Furthermore, the BALF positive results were equivalent to the microbiological culture positive results in terms of diagnostic value.

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