scholarly journals Synchrotron FTIR spectromicroscopy as a tool for studying populations and individual living cells of green algae

The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 7993-8001
Author(s):  
Kira L. Goff ◽  
Thomas H. Ellis ◽  
Kenneth E. Wilson
Keyword(s):  
Fourier Transform ◽  
Green Algae ◽  
Related Species ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Closely Related Species ◽  
Green Algal ◽  
Algal Genus

Fourier transform infrared (FTIR) spectromicroscopy was used to study variations in individual living cells of the green algal genus Chlamydomonas and to distinguuish between three closely-related species.

scholarly journals Synchrotron FTIR as a tool for studying populations and individual living cells of green algae

2019 ◽  
Author(s):  
Kira L. Goff ◽  
Thomas Ellis ◽  
Kenneth E. Wilson
Keyword(s):  
Green Algae ◽  
Related Species ◽  
Species Differences ◽  
Principal Component ◽  
Living Cells ◽  
Spectral Variation ◽  
Protein Profiles ◽  
Closely Related Species ◽  
Single Culture ◽  
Sources Of Variation

AbstractFourier transform infrared (FTIR) spectromicroscopy was used to study individual living cells of three closely-related species of the green algae Chlamydomonas. This study differentiated these three species based on differences in lipid and protein profiles, as well explored sources of variation in our measurements. Significant spectral variation was observed between individual cells within a single culture, as well as between control cultures of a species obtained on different days. Despite this, we were able to differentiate between the three close-related species. Differences in the spectra were confirmed using principal component analysis. Understanding the interplay of underlying variation and the degree of induced spectral differences is essential for the deployment of FTIR measurements in both bulk cultures and for individual living cells.

Synchrotron Fourier transform infrared (FTIR) analysis of single living cells progressing through the cell cycle

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 3891 ◽  
Author(s):  
Donna R. Whelan ◽  
Keith R. Bambery ◽  
Ljiljana Puskar ◽  
Don McNaughton ◽  
Bayden R. Wood
Keyword(s):  
Cell Cycle ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Ftir Analysis ◽  
Single Living Cells ◽  
Single Living

Protein hydration in living cells probed by Fourier transform infrared (FT-IR) spectroscopic imaging

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2475-2483 ◽  
Author(s):  
H. Shinzawa ◽  
B. Turner ◽  
J. Mizukado ◽  
S. G. Kazarian
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Protein Hydration ◽  
Ft Ir ◽  
Ir Spectroscopic

FT-IR spectra of a HEK cell were analyzed with 2D disrelation mapping to reveal molecular states of water and protein hydration.

scholarly journals Transmission Fourier Transform Infrared Spectroscopic Imaging, Mapping, and Synchrotron Scanning Microscopy with Zinc Sulfide Hemispheres on Living Mammalian Cells at Sub-Cellular Resolution

2020 ◽  
Vol 74 (5) ◽  
pp. 544-552 ◽  
Author(s):  
Ka Lung Andrew Chan ◽  
Ali Altharawi ◽  
Pedro Fale ◽  
Cai Li Song ◽  
Sergei G. Kazarian ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Synchrotron Radiation ◽  
Zinc Sulfide ◽  
Spatial Resolution ◽  
Single Point ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Imaging Systems ◽  
Ft Ir

Fourier transform infrared (FT-IR) spectroscopic imaging and microscopy of single living cells are established label-free technique for the study of cell biology. The constant driver to improve the spatial resolution of the technique is due to the diffraction limit given by infrared (IR) wavelength making subcellular study challenging. Recently, we have reported, with the use of a prototype zinc sulfide (ZnS) transmission cell made of two hemispheres, that the spatial resolution is improved by the factor of the refractive index of ZnS, achieving a λ/2.7 spatial resolution using the synchrotron–IR microscopy with a 36× objective with numerical aperture of 0.5. To refine and to demonstrate that the ZnS hemisphere transmission device can be translated to standard bench-top FT-IR imaging systems, we have, in this work, modified the device to achieve a more precise path length, which has improved the spectral quality of the living cells, and showed for the first time that the device can be applied to study live cells with three different bench-top FT-IR imaging systems. We applied focal plane array (FPA) imaging, linear array, and a synchrotron radiation single-point scanning method and demonstrated that in all cases, subcellular details of individual living cells can be obtained. Results have shown that imaging with the FPA detector can measure the largest area in a given time, while measurements from the scanning methods produced a smoother image. Synchrotron radiation single-point mapping produced the best quality image and has the flexibility to introduce over sampling to produce images of cells with great details, but it is time consuming in scanning mode. In summary, this work has demonstrated that the ZnS hemispheres can be applied in all three spectroscopic approaches to improve the spatial resolution without any modification to the existing microscopes.

FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

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