Synchrotron Fourier transform infrared (FTIR) analysis of single living cells progressing through the cell cycle

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 3891 ◽  
Author(s):  
Donna R. Whelan ◽  
Keith R. Bambery ◽  
Ljiljana Puskar ◽  
Don McNaughton ◽  
Bayden R. Wood
Keyword(s):  
Cell Cycle ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Ftir Analysis ◽  
Single Living Cells ◽  
Single Living

The Effect of Enucleation on Flagellar Regeneration in the Protozoon Peranema Trichophorum

1969 ◽  
Vol 4 (1) ◽  
pp. 171-178
Author(s):  
S. L. TAMM
Keyword(s):  
Cell Cycle ◽  
Mitotic Cycle ◽  
Living Cells ◽  
Regeneration Capacity ◽  
Flagellar Regeneration ◽  
Single Living Cells ◽  
Single Living

A rotocompressor was used to enucleate the flagellate protozoon Peranema trichophorum at known stages in the mitotic cycle. This new enucleation technique, combined with recently devised methods for amputating the flagellum and recording its regeneration in single living cells, permitted the investigation of the role of the nucleus in flagellar regeneration at different cell ages. The flagellar regeneration capacity of an enucleate Peranema depended on the stage in the cell cycle when the nucleus was removed. Post-division enucleate cells regenerated about half the length reached by sham-operated controls, and at slower rates, while predivision enucleate cells regenerated flagella equally as well as the controls. Therefore, the nucleus is making an immediate contribution to flagellar regeneration early in the cell cycle, but not late in the cell cycle.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

scholarly journals Synthesis of Novel Arginine-Based Flame Retardant and Its Application in Lyocell Fabric

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3588
Author(s):  
Jiayi Chen ◽  
Yansong Liu ◽  
Jiayue Zhang ◽  
Yuanlin Ren ◽  
Xiaohui Liu
Keyword(s):  
Fourier Transform ◽  
Heat Release ◽  
Flame Retardant ◽  
Photoelectron Spectroscopy ◽  
Fourier Transform Infrared ◽  
Ftir Analysis ◽  
Excellent Thermal Stability ◽  
X Ray ◽  
Cone Calorimeter Test ◽  
Before And After

Lyocell fabrics are widely applied in textiles, however, its high flammability increases the risk of fire. Therefore, to resolve the issue, a novel biomass-based flame retardant with phosphorus and nitrogen elements was designed and synthesized by the reaction of arginine with phosphoric acid and urea. It was then grafted onto the lyocell fabric by a dip-dry-cure technique to prepare durable flame-retardant lyocell fabric (FR-lyocell). X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated that the flame retardant was successfully introduced into the lyocell sample. Thermogravimetric (TG) and Raman analyses confirmed that the modified lyocell fabric featured excellent thermal stability and significantly increased char residue. Vertical combustion results indicated that FR-lyocell before and after washing formed a complete and dense char layer. Thermogravimetric Fourier-transform infrared (TG-FTIR) analysis suggested that incombustible substances (such as H2O and CO2) were produced and played a significant fire retarding role in the gas phase. The cone calorimeter test corroborated that the peak of heat release rate (PHRR) and total heat release (THR) declined by 89.4% and 56.4%, respectively. These results indicated that the flame retardancy of the lyocell fabric was observably ameliorated.

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