scholarly journals Dynamic monitoring of a bi-enzymatic reaction at a single biomimetic giant vesicle

The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 7922-7931
Author(s):  
Pauline Lefrançois ◽  
Bertrand Goudeau ◽  
Stéphane Arbault
Keyword(s):  
Confocal Microscopy ◽  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Enzymatic Reaction ◽  
Dynamic Monitoring ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Giant Vesicle ◽  
Micro Reactors

Giant unilamellar vesicles were used as individual biomimetic micro-reactors wherein a model bi-enzymatic reaction involving a glucose oxidase (GOx) and horseradish peroxidase (HRP) was monitored by confocal microscopy.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

scholarly journals Self-division of giant vesicles driven by an internal enzymatic reaction

2020 ◽  
Vol 11 (12) ◽  
pp. 3228-3235 ◽  
Author(s):  
Ylenia Miele ◽  
Zsófia Medveczky ◽  
Gábor Holló ◽  
Borbála Tegze ◽  
Imre Derényi ◽  
...  
Keyword(s):  
Membrane Transport ◽  
Enzymatic Reaction ◽  
Chemical Species ◽  
Ph Sensitive ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Giant Vesicles

Giant unilamellar vesicles having pH-sensitive bilayers can undergo self-division triggered by an internal enzymatic (urea–urease) reaction coupled to a cross-membrane transport of the chemical species.

scholarly journals ENCAPSULATION OF HORSERADISH PEROXIDASE-GLUCOSE OXIDASE (HRP-GOx) IN SILICA AQUAGEL SYNTHESIZED FROM RICE HULL ASH FOR ENZYMATIC REACTION OF GLUCOSE

2010 ◽  
Vol 8 (2) ◽  
pp. 169-176
Author(s):  
Nuryono Nuryono ◽  
Narsito Narsito ◽  
Endang Astuti
Keyword(s):  
Horseradish Peroxidase ◽  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Sol Gel ◽  
Enzymatic Reaction ◽  
Weight Ratio ◽  
Sodium Concentration ◽  
Rice Hull ◽  
Rice Hull Ash ◽  
Activity Decrease

In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalytical applications as well as biochemical and biophysical studies. In this research, encapsulation of Horseradish peroxidase-Glucose oxidase (HRP-GOx) enzymes in silica aquagel from rice hull ash by sol-gel process has been carried out. In addition, the effect of several parameters (weight ratio of HRP to GOx, pH, temperature, sodium ion concentration) on enzyme activity was studied, as well. Rice hull ash, which was produced by ashing at 700 °C, was extracted it's silika by NaOH solution 1 M at 100 °C for two hours to produce sodium silikate (Na2SiO3) solution. The Na2SiO3 solution with pH of 13 was added with a strong cation exchanger resin, to produce sol solution with the pH of 4. Encapsulation was emphasized by mixing sol solution and phosphate buffer pH 7 containing HRP-GOx solution at volume ratio of buffer to sol solution 1:5. The mixture was transferred into 96-microwell plate and was aged for 24 hours. Enzymatic reaction was carried out by adding chromogenic solution of phenol and 4-aminoantipyrine (4-AAP) and b-D-glucose solution (as substrate) into the microwell. Enzymatic activity was examined by measuring absorbance of product solution at 490 nm with ELISA reader. Result of enzymatic activity for encapsulated enzymes (SGE) was compared to that for free enzymes (EB). Results showed that at the investigated condition, HRP-GOx enzymes gave high activity at weight ratio of HRP to GOx 10:1 and pH 7 for both SGE and EB. Encapsulation caused the enzymes activity decrease to 53.0±0.2 %. However, SGE was observed to be more stable on pH and temperature changes than EB. Study on the effect of sodium concentration showed that the increase of sodium concentration from 0.10 to 0.37 M decreased the enzymatic activity to 56±0.2%. Reusability test showed that the synthesized SGE was reusable with activity decrease of 60% within 23 days.   Keywords: rice hull ash, encapsulation, aquagel, horseradish peroxidase, glucose oxidase

Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

2011 ◽  
Vol 2 (7) ◽  
pp. 713-718 ◽  
Author(s):  
Silvia Nappini ◽  
Tamer Al Kayal ◽  
Debora Berti ◽  
Bengt Nordèn ◽  
Piero Baglioni
Keyword(s):  
Confocal Microscopy ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Triggered Release

scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

2020 ◽  
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Confocal Microscopy ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Bilayer Membranes ◽  
Bending Modulus ◽  
Contrast Microscopy ◽  
Thermally Driven

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

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