Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

2011 ◽  
Vol 2 (7) ◽  
pp. 713-718 ◽  
Author(s):  
Silvia Nappini ◽  
Tamer Al Kayal ◽  
Debora Berti ◽  
Bengt Nordèn ◽  
Piero Baglioni
Keyword(s):  
Confocal Microscopy ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Triggered Release

scholarly journals Dynamic monitoring of a bi-enzymatic reaction at a single biomimetic giant vesicle

The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 7922-7931
Author(s):  
Pauline Lefrançois ◽  
Bertrand Goudeau ◽  
Stéphane Arbault
Keyword(s):  
Confocal Microscopy ◽  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Enzymatic Reaction ◽  
Dynamic Monitoring ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Giant Vesicle ◽  
Micro Reactors

Giant unilamellar vesicles were used as individual biomimetic micro-reactors wherein a model bi-enzymatic reaction involving a glucose oxidase (GOx) and horseradish peroxidase (HRP) was monitored by confocal microscopy.

scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

2020 ◽  
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Confocal Microscopy ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Bilayer Membranes ◽  
Bending Modulus ◽  
Contrast Microscopy ◽  
Thermally Driven

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

scholarly journals Inter- and Intra-Plane Interactions Control the Existence of Macrodomains in Asymmetric Giant Unilamellar Vesicles

2021 ◽  
Vol 120 (3) ◽  
pp. 147a
Author(s):  
Thais A. Enoki ◽  
Haden L. Scott ◽  
Gerald W. Feigenson ◽  
Frederick A. Heberle
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles
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