Peptide-modified conducting polymer as a biofunctional surface: monitoring of cell adhesion and proliferation

RSC Advances ◽  
2014 ◽  
Vol 4 (96) ◽  
pp. 53411-53418 ◽  
Author(s):  
Gizem Oyman ◽  
Caner Geyik ◽  
Rukiye Ayranci ◽  
Metin Ak ◽  
Dilek Odaci Demirkol ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Conducting Polymer ◽  
Functional Surface ◽  
Promising Candidate ◽  
Surface Monitoring ◽  
Cell Adhesion And Proliferation

A designed bio-functional surface is a promising candidate forcell-culture-on-a-chipapplications.

Geometrically customizable alginate hydrogel nanofibers for cell culture platforms

2019 ◽  
Vol 7 (42) ◽  
pp. 6556-6563 ◽  
Author(s):  
Satoshi Fujita ◽  
Yuka Wakuda ◽  
Minori Matsumura ◽  
Shin-ichiro Suye
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Core Shell ◽  
Anisotropic Structure ◽  
Alginate Hydrogel ◽  
Cell Adhesion And Proliferation

Hydrogel nanofibers derived from alginate with an anisotropic structure fabricated by using core–shell electrospinning play an important role in cell adhesion and proliferation as the extracellular matrix (ECM).

scholarly journals Folic acid modified clay/polymer nanocomposites for selective cell adhesion

2014 ◽  
Vol 2 (37) ◽  
pp. 6412-6421 ◽  
Author(s):  
F. B. Barlas ◽  
D. Ag Seleci ◽  
M. Ozkan ◽  
B. Demir ◽  
M. Seleci ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Folic Acid ◽  
Polymer Nanocomposites ◽  
Promising Material ◽  
Modified Clay ◽  
A Cell ◽  
Cell Adhesion And Proliferation

A promising material, a folic acid modified poly(epsilon-caprolactone)/clay nanocomposite that allows selective cell adhesion and proliferation, was synthesized and characterized as a cell culture and biosensing platform.

scholarly journals Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3042
Author(s):  
Eun Ju Lee ◽  
Khurshid Ahmad ◽  
Shiva Pathak ◽  
SunJu Lee ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Adhesion ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Primary Cells ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Promote Cell Adhesion

In recent years, a major rise in the demand for biotherapeutic drugs has centered on enhancing the quality and efficacy of cell culture and developing new cell culture techniques. Here, we report fibronectin (FN) derived, novel peptides fibronectin-based intergrin binding peptide (FNIN)2 (18-mer) and FNIN3 (20-mer) which promote cell adhesion proliferation, and the differentiation of primary cells and stem cells. FNIN2 and 3 were designed based on the in silico interaction studies between FN and its receptors (integrin α5β1, αvβ3, and αIIbβ3). Analysis of the proliferation of seventeen-cell types showed that the effects of FNINs depend on their concentration and the existence of expressed integrins. Significant rhodamine-labeled FNIN2 fluorescence on the membranes of HeLa, HepG2, A498, and Du145 cells confirmed physical binding. Double coating with FNIN2 or 3 after polymerized dopamine (pDa) or polymerized tannic acid (pTA) precoating increased HBEpIC cell proliferation by 30–40 percent, suggesting FNINs potently affect primary cells. Furthermore, the proliferation of C2C12 myoblasts and human mesenchymal stem cells (MSCs) treated with FNINs was significantly increased in 2D/3D culture. FNINs also promoted MSC differentiation into osteoblasts. The results of this study offer a new approach to the production of core materials (e.g., cell culture medium components, scaffolds) for cell culture.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Effect of Surface Potential on NIH3T3 Cell Adhesion and Proliferation

2014 ◽  
Vol 118 (26) ◽  
pp. 14464-14470 ◽  
Author(s):  
Hsun-Yun Chang ◽  
Chih-Chieh Huang ◽  
Kang-Yi Lin ◽  
Wei-Lun Kao ◽  
Hua-Yang Liao ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Surface Potential ◽  
Nih3t3 Cell ◽  
Cell Adhesion And Proliferation ◽  
Effect Of Surface

scholarly journals Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alice C. Taylor ◽  
Citlali Helenes González ◽  
Benjamin S. Miller ◽  
Robert J. Edgington ◽  
Patrizia Ferretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Neural Stem Cell ◽  
Human Neural Stem Cell ◽  
Surface Functionalisation ◽  
Cell Adhesion And Proliferation

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

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