scholarly journals Embedding of superparamagnetic iron oxide nanoparticles into membranes of well-defined poly(ethylene oxide)-block-poly(ε-caprolactone) nanoscale magnetovesicles as ultrasensitive MRI probes of membrane bio-degradation

2019 ◽  
Vol 7 (30) ◽  
pp. 4692-4705 ◽  
Author(s):  
Adeline Hannecart ◽  
Dimitri Stanicki ◽  
Luce Vander Elst ◽  
Robert N. Muller ◽  
Annie Brûlet ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Ethylene Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Polymer Vesicles ◽  
Nanoprecipitation Method ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

The present study reports the preparation of PEO-b-PCL polymer vesicles via a nanoprecipitation method and the loading of hydrophobically coated USPIO into the membrane of these nanovesicles, whose thickness was measured precisely by SANS.

scholarly journals Clustering of Iron Oxide Nanoparticles into Poly(ethylene oxide)-block-poly(ε-caprolactone) Nanoassemblies as Ultrasensitive MRI Probes

2016 ◽  
Author(s):  
Adeline Hannecart ◽  
Dimitri Stanicki ◽  
Luce Vander Elst ◽  
Laetitia Mespouille ◽  
Robert N. Muller ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Ethylene Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

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